Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,381,934	A→G	intergenic (+32/‑13)	ycjV → / → ompG	pseudogene; putative ATP‑binding component of a transport system/outer membrane porin G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,381,934	0	A	G	100.0%	210.7 / NA	57	intergenic (+32/‑13)	ycjV/ompG	pseudogene; putative ATP‑binding component of a transport system/outer membrane porin G
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/29);  total (28/29)

TAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCG  >  NC_000913/1381787‑1382079                                                                                                                                                    |                                                                                                                                                  tAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCa                                                                                                                                                 >  2:107894/1‑150 (MQ=255)          gTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGCGAAGGCATTCCCCCATGATAATGCAAGGAACaata                                                                                                                                       <  2:703087/151‑1 (MQ=255)            gCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACaataat                                                                                                                                     >  1:152842/1‑151 (MQ=255)            gCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACaataat                                                                                                                                     <  1:525216/151‑1 (MQ=255)            gCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACaataa                                                                                                                                      <  1:156761/150‑1 (MQ=255)              tGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATg                                                                                                                                    >  1:623583/1‑150 (MQ=255)               ggTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATg                                                                                                                                    <  1:304405/149‑1 (MQ=255)                 tGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGaaa                                                                                                                                 >  1:446527/1‑150 (MQ=255)                  gCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGaaaaa                                                                                                                               <  1:532359/151‑1 (MQ=255)                   cGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGaaaa                                                                                                                                >  2:804244/1‑149 (MQ=255)                          tGATTACCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTAc                                                                                                                       >  2:858043/1‑151 (MQ=255)                           gATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTAc                                                                                                                       <  2:61858/150‑1 (MQ=255)                           gATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTAc                                                                                                                       >  1:800933/1‑150 (MQ=255)                            aTTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTAccc                                                                                                                     >  2:459717/1‑151 (MQ=255)                                tCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGAAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGt                                                                                                                  >  2:770609/1‑150 (MQ=255)                                   tGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCg                                                                                                              >  2:31609/1‑151 (MQ=255)                                      aGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCAc                                                                                                           >  1:274729/1‑151 (MQ=255)                                       ggggaAAATATCACTATTCATTTTGATATGAAGAAATGTCATTTCGTTAATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTACCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCATGTACCGCACt                                                                                                          >  1:209964/4‑151 (MQ=255)                                       ggAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACt                                                                                                          <  1:119323/151‑1 (MQ=255)                                        gagaAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTg                                                                                                         <  1:230991/151‑1 (MQ=255)                                          gaAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGt                                                                                                       <  2:413382/151‑1 (MQ=255)                                               tatCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGAtgt                                                                                                  >  2:185844/1‑151 (MQ=255)                                                atCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGAtgtg                                                                                                 <  2:429555/151‑1 (MQ=255)                                                   aCTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCC‑GGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCg                                                                                             >  1:369151/1‑151 (MQ=255)                                                      aTTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCggg                                                                                           >  2:94080/1‑151 (MQ=255)                                                      aTTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCggg                                                                                           >  1:345802/1‑151 (MQ=255)                                                      aTTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCgg                                                                                            <  2:430785/150‑1 (MQ=255)                                                      aTTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCgg                                                                                            <  2:748015/150‑1 (MQ=255)                                                       ttCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCggg                                                                                           >  2:8297/1‑150 (MQ=255)                                                               gATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAACGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTg                                                                                  <  2:51346/151‑1 (MQ=255)                                                                 tatGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTgcg                                                                                >  2:664093/1‑151 (MQ=255)                                                                  atGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTgcgc                                                                               <  1:323164/151‑1 (MQ=255)                                                                         aaTGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGcc                                                                        >  2:198736/1‑151 (MQ=255)                                                                                         tGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACt                                                        >  1:649463/1‑151 (MQ=255)                                                                                            aGAAACGGAAATAGCAATTCGTTAAATACAGGGGGAAGGCATTCCCTCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCAGAGGCTGAGGGAAGGAACGACTg                                                       >  1:737724/1‑149 (MQ=255)                                                                                            aGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGc                                                     <  1:80360/151‑1 (MQ=255)                                                                                             gAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCgg                                                                                            >  2:808171/1‑111 (MQ=255)                                                                                             gAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCgg                                                                                            <  1:808115/111‑1 (MQ=255)                                                                                              aaaCGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCAc                                                   <  2:544474/151‑1 (MQ=255)                                                                                                  ggAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTa                                               <  1:765558/151‑1 (MQ=255)                                                                                                    aaaTAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAat                                             <  1:31587/151‑1 (MQ=255)                                                                                                    aaaTAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAat                                             <  2:169990/151‑1 (MQ=255)                                                                                                    aaaTAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAat                                             >  1:13135/1‑151 (MQ=255)                                                                                                                         aGGGGGAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATa                        <  1:806944/151‑1 (MQ=255)                                                                                                                             ggAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGaaa                    <  2:345825/151‑1 (MQ=255)                                                                                                                             ggAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGaaa                    <  1:105327/151‑1 (MQ=255)                                                                                                                              gAAGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGaaaa                   <  2:346861/151‑1 (MQ=255)                                                                                                                               aaGGCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGTAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAAc                  <  1:390633/151‑1 (MQ=255)                                                                                                                                  gCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTc               >  2:582903/1‑151 (MQ=255)                                                                                                                                  gCATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTc               <  1:783676/151‑1 (MQ=255)                                                                                                                                   cATTCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCg              >  2:234968/1‑151 (MQ=255)                                                                                                                                      tCCCCCAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAg            <  2:133090/150‑1 (MQ=255)                                                                                                                                       cccccAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAggg          >  1:493580/1‑151 (MQ=255)                                                                                                                                         cccAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGtt        >  1:601015/1‑151 (MQ=255)                                                                                                                                           cAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTAt      <  2:736011/151‑1 (MQ=255)                                                                                                                                           cAGGATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAACAGAAAACGTCGAGGGTTAt      <  2:708419/151‑1 (MQ=255)                                                                                                                                              gATAATGCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGc   >  2:393272/1‑151 (MQ=255)                                                                                                                                               ataatgCAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCg  <  2:419185/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  TAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCGAGGGTTATGGCG  >  NC_000913/1381787‑1382079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑