Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,474,495	A→G	D92G (GAC→GGC)	ydbC →	putative NAD(P)‑binding oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,474,495	0	A	G	97.8%	151.0 / NA	45	D92G (GAC→GGC)	ydbC	putative NAD(P)‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (20/24);  minor base T (1/0);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGACGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATGGCCCAGCGGAAGGATC  >  NC_000913/1474347‑1474636                                                                                                                                                     |                                                                                                                                              cGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCg                                                                                                                                             <  2:425874/151‑1 (MQ=255)      ggCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATcc                                                                                                                                        <  1:796858/151‑1 (MQ=255)             gCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGt                                                                                                                                    <  1:49631/148‑1 (MQ=255)                  gggCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCa                                                                                                                            <  2:755864/151‑1 (MQ=255)                                   aTACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCt                                                                                                           >  2:248915/1‑151 (MQ=255)                                    tACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTg                                                                                                          >  1:482992/1‑151 (MQ=255)                                      ccAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGc                                                                                                         >  2:75538/1‑150 (MQ=255)                                      ccAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGc                                                                                                         >  1:61483/1‑150 (MQ=255)                                         gCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCaaaa                                                                                                     <  2:764848/151‑1 (MQ=255)                                         gCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCATTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCaaa                                                                                                      >  2:552821/1‑150 (MQ=255)                                               tttATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCg                                                                                                 <  1:176806/149‑1 (MQ=255)                                                  aTGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCa                                                                                            <  1:825221/151‑1 (MQ=255)                                                        cGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATaa                                                                                      <  1:7246/151‑1 (MQ=255)                                                        cGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATa                                                                                       <  2:633536/150‑1 (MQ=255)                                                           aCGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCt                                                                                   >  1:763860/1‑151 (MQ=255)                                                              tCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTAc                                                                                 <  2:512986/150‑1 (MQ=255)                                                               caccacTCAGATTATCCGCGAAGCGCTTTATTGTTACTCTGACGACGTGACAATTGTCGCTAAGATTTGTGCGCGGCGCGGAGAGGTCGCATCCTGGTTGCTCGCATCTTCTCTGTCAGAGCTGCAAAAAGCGGTGAACGATACTCTACGt                                                                               >  1:165997/1‑151 (MQ=255)                                                                   aaTCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAAtc                                                                           >  1:764039/1‑151 (MQ=255)                                                                     tCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAAtctc                                                                         >  1:322484/1‑151 (MQ=255)                                                                      cAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCg                                                                        >  1:547321/1‑151 (MQ=255)                                                                        gATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCggg                                                                      <  1:19416/151‑1 (MQ=255)                                                                        gATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCggg                                                                      <  1:362790/151‑1 (MQ=255)                                                                          ttATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCt                                                                    >  1:225751/1‑151 (MQ=255)                                                                                gcgAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGt                                                              <  2:753220/151‑1 (MQ=255)                                                                                  aaaGCGCTTTCTCCTTACTCTGACGACCTGACAATTGTCACTAAAACTGGTGCGCGGCGTGGAGAGGGCCCATCCTGGATGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTCATCTCGGGCTGGAc                                                                >  1:556772/2‑147 (MQ=255)                                                                                          ttATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGAtgt                                                     <  2:130575/150‑1 (MQ=255)                                                                                            aTCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGt                                                  >  2:174560/1‑151 (MQ=255)                                                                                              ccTTACACTGACGACCTGACAATTGTCGCTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGtga                                                <  1:832811/151‑3 (MQ=255)                                                                                               cTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTa                                                >  2:213452/1‑150 (MQ=255)                                                                                                  aCTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCt                                            <  1:174537/151‑1 (MQ=255)                                                                                                    tctGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTgc                                          >  1:375041/1‑151 (MQ=255)                                                                                                       gacgacCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTgcg                                         <  1:683894/149‑1 (MQ=255)                                                                                                       gacgacCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGcgcg                                       >  1:443994/1‑151 (MQ=255)                                                                                                              tGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTatgat                                >  2:158504/1‑151 (MQ=255)                                                                                                                aCAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATgg                              <  1:620948/151‑1 (MQ=255)                                                                                                                             aaaTTGGTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATgg                 <  1:213428/151‑1 (MQ=255)                                                                                                                                  ggTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATGGCCCa             <  2:500345/150‑1 (MQ=255)                                                                                                                                   gTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATGGCCCAGc           >  1:835539/1‑151 (MQ=255)                                                                                                                                   gTGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATGGCCCAGc           <  2:71988/151‑1 (MQ=255)                                                                                                                                    tGCGCGGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATGGCCCAGCg          >  1:445993/1‑151 (MQ=255)                                                                                                                                      cgcgGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATGGCCCAGCGGa        <  2:818092/151‑1 (MQ=255)                                                                                                                                      cgcgGCGTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGAGGGTCATGGCCCAGCgg         <  1:636713/150‑1 (MQ=255)                                                                                                                                            gTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATGGCCCAGCGGAAGGATc  >  1:788041/1‑151 (MQ=255)                                                                                                                                            gTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATGGCCCAGCGGAAGGATc  <  2:415606/151‑1 (MQ=255)                                                                                                                                            gTGGAGAGGGCGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATGGCCCAGCGGAAGGATc  >  1:372391/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                              CGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGTCCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACAATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGACGCATCCTGGTTGCCCGCATTTTCTCCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTGGATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATGGCCCAGCGGAAGGATC  >  NC_000913/1474347‑1474636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑