Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,559,761	T→C	S50S (TCA→TCG)	ddpC ←	D,D‑dipeptide ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,559,761	0	T	C	100.0%	166.0 / NA	48	S50S (TCA→TCG)	ddpC	D,D‑dipeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/30);  total (18/30)

GGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTGCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATCCATACCAGTTTTGCACCGTTAAATCGCGTTTGTTTTTGTGGGCGTACGGCGGACGTTTCCTCGCTTAGCATC  >  NC_000913/1559614‑1559908                                                                                                                                                    |                                                                                                                                                    ggCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTGCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGaa                                                                                                                                                  <  1:322634/151‑1 (MQ=255)  gCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTGCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGaaa                                                                                                                                                 <  2:179179/151‑1 (MQ=255)   cGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTGCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGaaa                                                                                                                                                 >  2:777635/1‑150 (MQ=255)    gagaATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTGCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGaaaaa                                                                                                                                               <  2:176967/151‑1 (MQ=255)      gaATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTGCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAat                                                                                                                                             <  1:154941/151‑1 (MQ=255)           gattgCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTGCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAatcatc                                                                                                                                         <  2:57326/150‑1 (MQ=255)              tgCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTGCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAatcatcatc                                                                                                                                      <  2:584068/150‑1 (MQ=255)                 tGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTGCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAAt                                                                                                                                   >  2:228958/1‑150 (MQ=255)                  gACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTGCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATa                                                                                                                                  <  1:277091/150‑1 (MQ=255)                               tACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTGCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAatg                                                                                                                    >  2:250043/1‑151 (MQ=255)                                   cgGCTAAACAGATCGCGTCCCACTTCATCGGTGCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAAtgatga                                                                                                                <  2:25829/151‑1 (MQ=255)                                      cTAAACAGATCGCGTCCCACTTCATCGGTGCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAAtgatga                                                                                                                >  2:555503/1‑148 (MQ=255)                                           cAGATCGCGTCCCACTTCATCGGTGCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCt                                                                                                         <  1:196510/150‑1 (MQ=255)                                                cgcgTCCCACTTCATCGGTGCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCTGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCAc                                                                                                   <  1:636879/151‑1 (MQ=255)                                                 gcgTCCCACTTCATCGGTGCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACg                                                                                                  >  2:559244/1‑151 (MQ=255)                                                  cgTCCCACTTCATCGGTGCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACgg                                                                                                 >  2:544564/1‑151 (MQ=255)                                                            cATCGGTGCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGAc                                                                                        <  2:28279/150‑1 (MQ=255)                                                               cGGTGCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGcc                                                                                    >  2:514653/1‑151 (MQ=255)                                                                   gCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCttt                                                                                 <  2:654846/150‑1 (MQ=255)                                                                     cAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTc                                                                                >  2:505845/1‑149 (MQ=255)                                                                     cAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTc                                                                                >  1:605694/1‑149 (MQ=255)                                                                      aaaCCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGc                                                                             >  2:8070/1‑151 (MQ=255)                                                                        aCCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCAt                                                                           <  1:575594/151‑1 (MQ=255)                                                                              gcgcCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATCCATa                                                                      <  1:506199/150‑1 (MQ=255)                                                                              gcgcCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATCCATAc                                                                     <  2:557483/151‑1 (MQ=255)                                                                              gcgcCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATCCATAc                                                                     <  1:42112/151‑1 (MQ=255)                                                                                   gcAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATCCATACCAGtt                                                                <  1:474957/151‑1 (MQ=255)                                                                                     aGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATCCATACCAGtttt                                                              <  2:682649/151‑1 (MQ=255)                                                                                      gAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATCCATACCAGTTTTg                                                             <  2:252613/151‑1 (MQ=255)                                                                                         ggcggcAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATCCATACCAGTTTTGCAc                                                          <  1:548943/151‑1 (MQ=255)                                                                                             gcAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATCCATACCAGTTTTGCACCGtt                                                      <  2:281790/151‑1 (MQ=255)                                                                                                aaaGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATCCATACCAGTTTTGCACCGTTaaa                                                   <  1:320547/151‑1 (MQ=255)                                                                                                 aaGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATCCATACCAGTTTTGCACCGTTaaa                                                   >  1:151493/1‑150 (MQ=255)                                                                                                  aGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATCCATACCAGTTTTGCACCGTTAAATc                                                 >  2:221874/1‑151 (MQ=255)                                                                                                  aGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATCCATACCAGTTTTGCACCGTTAAATc                                                 >  2:548615/1‑151 (MQ=255)                                                                                                      gggcggTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATCCATACCAGTTTTGCACCGTTAAATCGCgt                                             <  2:260314/151‑1 (MQ=255)                                                                                                          ggTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATCCATACCAGTTTTGCACCGTTAAATCGCgtttgt                                         <  2:288323/151‑1 (MQ=255)                                                                                                           gTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATcc                                                                         >  2:404659/1‑118 (MQ=255)                                                                                                           gTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATcc                                                                         <  1:404627/118‑1 (MQ=255)                                                                                                           gTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATCCATACCAGTTTTGCACCGTTAAATCGCgtttgtt                                        >  2:299983/1‑151 (MQ=255)                                                                                                                        cGTTGGGATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATCCATACCAGTTTTGCACCGTTAAATCGCGTTTGTTTTTGTGGGCGTAc                           >  1:242857/1‑151 (MQ=255)                                                                                                                            gggATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATCCATACCAGTTTTGCACCGTTAAATCGCGTTTGTTTTTGTGGGCGTAcggcg                       <  1:232430/151‑1 (MQ=255)                                                                                                                              gATCATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATCCATACCAGTTTTGCACCGTTAAATCGCGTTTGTTTTTGTGGGCGTAcggcgg                      >  2:227212/1‑150 (MQ=255)                                                                                                                                 cATGCGTCGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATCCATACCAGTTTTGCACCGTTAAATCGCGTTTGTTTTTGTGGGCGTACGGCGGACGt                  <  1:92955/151‑1 (MQ=255)                                                                                                                                        cGCCAGCCACGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATCCATACCAGTTTTGCACCGTTAAATCGCGTTTGTTTTTGTGGGCGTACGGCGGACGTTTCCTc            <  1:159486/150‑1 (MQ=255)                                                                                                                                           cagccaCGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATCCATACCAGTTTTGCACCGTTAAATCGCGTTTGTTTTTGTGGGCGTACGGCGGACGTTTCCTCGCtt        <  2:567695/151‑1 (MQ=255)                                                                                                                                              ccaCGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATCCATACCAGTTTTGCACCGTTAAATCGCGTTTGTTTTTGTGGGCGTACGGCGGACGTTTCCTCGCTTAGc     <  1:628961/151‑1 (MQ=255)                                                                                                                                                 cGGCGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATCCATACCAGTTTTGCACCGTTAAATCGCGTTTGTTTTTGTGGGCGTACGGCGGACGTTTCCTCGCTTAGcatc  >  1:404846/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GGCGAGAATTGATTGCTGACTGCCGACCAGTACGCGGCTAAACAGATCGCGTCCCACTTCATCGGTGCCAAACCAGTGCGCCGCAGAAGGCGGCAAAAGGCGGGCGGTTAAATCAATGGCGTTGGGATCATGCGTCGCCAGCCACGGTGAAAAAATCATCATCAATAGCATTAATACAATGATGACTGCGCTGGTCACGGTGAGCGGACTGCCTTTCAGCATCCATACCAGTTTTGCACCGTTAAATCGCGTTTGTTTTTGTGGGCGTACGGCGGACGTTTCCTCGCTTAGCATC  >  NC_000913/1559614‑1559908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑