Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,582,048	A→G	G159G (GGT→GGC)	ydeN ←	putative Ser‑type periplasmic non‑aryl sulfatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,582,048	0	A	G	100.0%	174.5 / NA	47	G159G (GGT→GGC)	ydeN	putative Ser‑type periplasmic non‑aryl sulfatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/22);  total (25/22)

TGGTGAAGTTGTCATGATAGTCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCG  >  NC_000913/1581900‑1582193                                                                                                                                                     |                                                                                                                                                  tggtgAAGTTGTCATGATAGTCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGcc                                                                                                                                                 >  2:425514/1‑151 (MQ=255)     gAAGTTGTCATGATAGTCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATcc                                                                                                                                             >  1:848488/1‑151 (MQ=255)     gAAGTTGTCATGATAGTCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATcc                                                                                                                                             >  1:500790/1‑151 (MQ=255)      aaGTTGTCATGATAGTCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCt                                                                                                                                            <  1:779557/151‑1 (MQ=255)           gTCATGATAGTCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGc                                                                                                                                        <  1:100556/150‑1 (MQ=255)            tCATGATAGTCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAg                                                                                                                                         <  1:159223/148‑1 (MQ=255)            tCATGATAGTCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAg                                                                                                                                         >  2:159246/1‑148 (MQ=255)               tGATAGTCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCgg                                                                                                                                   >  1:358950/1‑151 (MQ=255)                 aTAGTCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTa                                                                                                                                 <  1:76704/151‑1 (MQ=255)                   aGTCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTAtt                                                                                                                               <  2:582079/151‑1 (MQ=255)                   aGTCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTAt                                                                                                                                <  1:593521/150‑1 (MQ=255)                        cgcgTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAAt                                                                                                                          <  2:731881/151‑1 (MQ=255)                             ttgtttATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGac                                                                                                                      <  1:620069/150‑1 (MQ=255)                                       tCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAg                                                                                                               <  1:422600/147‑1 (MQ=255)                                             aCCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGcgggcggg                                                                                                       >  1:500074/1‑149 (MQ=255)                                                  cacaTTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCg                                                                                                 >  1:589579/1‑150 (MQ=255)                                                  cacaTTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGa                                                                                                <  2:550748/151‑1 (MQ=255)                                                    caTTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGAcc                                                                                              >  1:211783/1‑151 (MQ=255)                                                     aTTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCg                                                                                             >  2:290463/1‑151 (MQ=255)                                                          tGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCat                                                                                        >  1:651607/1‑151 (MQ=255)                                                           gATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCatt                                                                                       >  1:68887/1‑151 (MQ=255)                                                           gATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCa                                                                                         <  2:851955/149‑1 (MQ=255)                                                             tttttGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCattat                                                                                     >  1:270284/1‑151 (MQ=255)                                                                   aCAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTgcggc                                                                               >  2:313098/1‑151 (MQ=255)                                                                      aGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGcgcg                                                                            >  1:7678/1‑151 (MQ=255)                                                                            aTTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAggg                                                                      >  2:298656/1‑151 (MQ=255)                                                                                 ccTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCg                                                                  <  1:783911/150‑1 (MQ=255)                                                                                    aCTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGaa                                                               >  2:763027/1‑150 (MQ=255)                                                                                    aCTGCTGCAGTGTAATAACCATGAATCTGGAATAATTCAAGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTTAGCATCGGTATTGGAATAGACCCCAAAGCAGGCGGGAGCGCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGaa                                                               <  2:555722/150‑1 (MQ=255)                                                                                       gctgcAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAac                                                             <  2:105838/149‑1 (MQ=255)                                                                                           cAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCgtg                                                       <  2:417373/151‑1 (MQ=255)                                                                                                   taaCCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACAt                                               >  1:254269/1‑151 (MQ=255)                                                                                                    aaCCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATa                                              <  2:175051/151‑1 (MQ=255)                                                                                                        aTGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCg                                          >  2:830962/1‑151 (MQ=255)                                                                                                           aTTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTa                                       <  1:228632/151‑1 (MQ=255)                                                                                                                        tCAGGCAAGAAAGTTTCTGTTAGTGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTATAACGTACGc                          >  2:317900/1‑151 (MQ=255)                                                                                                                         cAGGCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGcc                         <  1:149307/151‑1 (MQ=255)                                                                                                                           ggCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCtt                       <  1:231167/151‑1 (MQ=255)                                                                                                                            gCAAGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTc                      >  2:790084/1‑151 (MQ=255)                                                                                                                              aaGAAAGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCAt                    <  1:520672/151‑1 (MQ=255)                                                                                                                                   aGTTTCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCAtt               >  2:314935/1‑151 (MQ=255)                                                                                                                                     tttCTGTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTa              >  1:710869/1‑150 (MQ=255)                                                                                                                                          gTTAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGaa         >  1:319268/1‑150 (MQ=255)                                                                                                                                            tAGCGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAg       <  1:568590/150‑1 (MQ=255)                                                                                                                                               cGGAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGc   <  2:734821/151‑1 (MQ=255)                                                                                                                                                ggAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGGAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCg  >  2:309057/1‑151 (MQ=255)                                                                                                                                                 gAATGCCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCg  >  1:673199/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                  TGGTGAAGTTGTCATGATAGTCACGCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCG  >  NC_000913/1581900‑1582193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑