Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	233,559	T→C	T133A (ACG→GCG)	mltD ←	putative membrane‑bound lytic murein transglycosylase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	233,559	0	T	C	100.0%	219.5 / NA	64	T133A (ACG→GCG)	mltD	putative membrane‑bound lytic murein transglycosylase D
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/37);  total (27/37)

GTTCAGACGCTGCATCATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGTTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGGAGATAGCTCTTATTGCGTAA  >  NC_000913/233413‑233703                                                                                                                                                   |                                                                                                                                                 gTTCAGACGCTGCATCATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCg                                                                                                                                              <  2:71671/151‑1 (MQ=255)    cAGACGCTGCATCATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCg                                                                                                                                              >  1:387038/1‑148 (MQ=255)     aGACGCTGCATCATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTg                                                                                                                                            >  2:171113/1‑149 (MQ=255)         gCTGCATCATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATc                                                                                                                                      <  2:414158/151‑1 (MQ=255)         gCTGCATCATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATc                                                                                                                                      <  1:266413/151‑1 (MQ=255)            gCATCATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCaa                                                                                                                                    >  2:5319/1‑150 (MQ=255)             catcatATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCaaaa                                                                                                                                  <  1:380363/151‑1 (MQ=255)                      cAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTcc                                                                                                                         <  1:384356/151‑1 (MQ=255)                            ggCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATg                                                                                                                   <  1:214858/151‑1 (MQ=255)                             gCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATgg                                                                                                                  <  1:665385/151‑1 (MQ=255)                              cAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATggg                                                                                                                 >  1:119182/1‑151 (MQ=255)                                       aaGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTAc                                                                                                        <  2:231308/151‑1 (MQ=255)                                        aGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTAcc                                                                                                       >  2:529269/1‑151 (MQ=255)                                        aGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTAcc                                                                                                       >  1:272322/1‑151 (MQ=255)                                           aacaacATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGt                                                                                                    >  2:424931/1‑151 (MQ=255)                                              aacaTCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTcc                                                                                                 >  2:36841/1‑151 (MQ=255)                                               acaTCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCa                                                                                                <  2:336741/151‑1 (MQ=255)                                                   cgcgACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAgg                                                                                            <  2:425143/151‑1 (MQ=255)                                                   cgcgACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAgg                                                                                            <  1:747788/151‑1 (MQ=255)                                                            cgTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACg                                                                                   >  2:651273/1‑151 (MQ=255)                                                                aTAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGtttt                                                                               >  1:432557/1‑151 (MQ=255)                                                                 tAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGttttt                                                                              >  2:303542/1‑151 (MQ=255)                                                                  aaTTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGtttttt                                                                             <  2:733009/151‑1 (MQ=255)                                                                  aaTTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGttttt                                                                              <  2:57768/150‑1 (MQ=255)                                                                   aTTGCGGGTCTGTTTCAAACCATAATTGCGCCCAGTGATCGGAATGATCTGCCAGATGCCTGCGGCATAGGCGCCCGACGCTGCGTGAGGATCAAAAGCGCCCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGGTACGtttttt                                                                             >  1:198727/1‑150 (MQ=255)                                                                       cGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACtt                                                                        <  1:673549/151‑1 (MQ=255)                                                                               tttCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCt                                                                >  2:53628/1‑151 (MQ=255)                                                                               tttCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCt                                                                >  1:564540/1‑151 (MQ=255)                                                                                   aaaCCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATcc                                                            <  2:459087/151‑1 (MQ=255)                                                                                     aCCATGATTGTGCCCCGTGCTGGGAATGAGCTGCCAGACGCCTGGGGCGTTGGCGCGAGACGCTGCGTGAGGATCAATGGCGCTCTCCACTATGGGTAGTAGTAACAGTTCCATAGGCATGTTACGTTTTTTAATTTGCCGTGCTa                                                               <  1:830193/146‑1 (MQ=255)                                                                                     aCCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATcc                                                            >  2:124561/1‑149 (MQ=255)                                                                                     aCCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATcc                                                            >  2:831420/1‑149 (MQ=255)                                                                                     aCCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAg                                                          <  1:529232/151‑1 (MQ=255)                                                                                       cATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTa                                                        <  1:235124/151‑1 (MQ=255)                                                                                        aTAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTAc                                                       <  2:35409/151‑1 (MQ=255)                                                                                          aaTTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACat                                                     >  1:367252/1‑151 (MQ=255)                                                                                           aTTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACata                                                    <  2:389271/151‑1 (MQ=255)                                                                                           aTTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACat                                                     >  1:829942/1‑150 (MQ=255)                                                                                              gcgcCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATAc                                                 <  2:357767/151‑1 (MQ=255)                                                                                               cgcCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACg                                                <  2:804727/151‑1 (MQ=255)                                                                                                       gCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGcc                                        <  2:423866/151‑1 (MQ=255)                                                                                                       gCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGcc                                        >  1:152020/1‑151 (MQ=255)                                                                                                           ggAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCg                                      >  1:82755/1‑149 (MQ=255)                                                                                                           ggAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGt                                     >  1:616993/1‑150 (MQ=255)                                                                                                            gAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTa                                    >  2:693742/1‑150 (MQ=255)                                                                                                                    tGCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACAt                           >  2:64363/1‑151 (MQ=255)                                                                                                                     gCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGcc                                        <  2:162806/137‑1 (MQ=255)                                                                                                                     gCCAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGcc                                        >  1:162783/1‑137 (MQ=255)                                                                                                                       cAGATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGt                        <  1:522419/151‑1 (MQ=255)                                                                                                                         gATGCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTgg                      <  2:113877/151‑1 (MQ=255)                                                                                                                            gCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGgag                    <  1:677917/150‑1 (MQ=255)                                                                                                                            gCCTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGgag                    <  2:795990/150‑1 (MQ=255)                                                                                                                             ccTGCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGGAGAt                  <  2:410283/151‑1 (MQ=255)                                                                                                                                gCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGGAGATAg                <  2:357659/150‑1 (MQ=255)                                                                                                                                gCGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGGAGATAg                <  2:118114/150‑1 (MQ=255)                                                                                                                                 cGGCATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGGAGATAGct              <  1:801354/151‑1 (MQ=255)                                                                                                                                    cATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGGAGATAGCTCtt           <  2:243972/151‑1 (MQ=255)                                                                                                                                    cATTGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGGAGATAGCTCtt           >  2:28934/1‑151 (MQ=255)                                                                                                                                       tGGCGCCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGGAGATAGCTCTTAt         <  1:36819/150‑1 (MQ=255)                                                                                                                                         gcgcCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTATGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGGAGATAGCTCTTATTGc      <  1:212895/151‑1 (MQ=255)                                                                                                                                          cgcCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGGAGATAGCTCTTATTGCg     >  2:22614/1‑151 (MQ=255)                                                                                                                                           gcCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGGAGATAGCTCTTATTGCGt    <  1:624219/151‑1 (MQ=255)                                                                                                                                           gcCAGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGGAGATAGCTCTTATTGCGt    <  1:716009/151‑1 (MQ=255)                                                                                                                                              aGACGCTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGGAGATAGCTCTTATTGCGTaa  >  1:203545/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                 GTTCAGACGCTGCATCATATTCAGCGCGGCAGTTGTTGAAGCAACAACATCGCGACGCGCGTCATAATTGCGGGTCTGTTTCAAACCATAATTGCGCCCCGTGCTCGGAATGATCTGCCAGATGCCTGCGGCATTGGCGCCAGACGTTGCGTGAGGATCAAAAGCGCTCTCCACTATGGGTAGTAGTACCAGTTCCATAGGCATGTTACGTTTTTTAACTTGCCCTGCTATCCAGTACATATACGGCTCTGCCCGTAAAGTTACATCGTGGAGATAGCTCTTATTGCGTAA  >  NC_000913/233413‑233703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑