Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,583,067 A→G C207C (TGT→TGC ydeO ← UV‑inducible global regulator, EvgA‑, GadE‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,583,0670AG98.8% 286.0 / NA 81C207C (TGT→TGCydeOUV‑inducible global regulator, EvgA‑, GadE‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (34/46);  total (34/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

AAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACCCGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCACATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCTTTCACTGATGTACAGGCA  >  NC_000913/1582920‑1583213
                                                                                                                                                   |                                                                                                                                                  
aaaTAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACCCGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCAt                                                                                                                                                 <  2:836490/151‑1 (MQ=255)
  ataatCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACCCGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTg                                                                                                                                               <  1:824965/151‑1 (MQ=255)
      tCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACCCGTGTGACTTTGACATCGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCCAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATCCCCGCATttttt                                                                                                                                            <  1:480396/150‑2 (MQ=255)
      tCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACCCGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCg                                                                                                                                           >  2:504434/1‑151 (MQ=255)
       aaaaTAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACCCGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCgg                                                                                                                                          >  1:274722/2‑151 (MQ=255)
         aaTAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACCCGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCa                                                                                                                                        <  2:459014/151‑1 (MQ=255)
         aaTAGCTAAAGCATGCATCGTGTTGCCCGTATTCATACCCGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGTCATAACCGCATTGTTCTGCa                                                                                                                                        <  1:460/151‑1 (MQ=255)
          aTAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACCCGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCaa                                                                                                                                       <  2:416627/151‑1 (MQ=255)
               tAAAGCATTCATCGTGTTGCCCGTATTCATACCCGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAAtttt                                                                                                                                   >  2:17774/1‑150 (MQ=255)
               tAAAGCATTCATCGTGTTGCCCGTATTCATACCCGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAAtttt                                                                                                                                   >  1:431677/1‑150 (MQ=255)
                aaaGCATTCATCGTGTTGCCCGTATTCATACCCGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAAtttt                                                                                                                                   <  2:758398/149‑1 (MQ=255)
                aaaGCATTCATCGTGTTGCCCGTATTCATACCCGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAAtttt                                                                                                                                   <  2:536112/149‑1 (MQ=255)
                aaaGCATTCATCGTGTTGCCCGTATTCATACCCGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTAt                                                                                                                                 <  2:842131/151‑1 (MQ=255)
                aaaGCATTCATCGTGTTGCCCGTATTCATACCCGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCACATTGTTCGGCAATTTTAt                                                                                                                                 <  2:754946/151‑1 (MQ=255)
                 aaGCATTCATCGTGTTGCCCGTATTCATACCCGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTAtt                                                                                                                                >  2:676754/1‑151 (MQ=255)
                 aaGCATTCATCGTGTTGCCCGTATTCATACCCGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTAt                                                                                                                                 >  1:782279/1‑150 (MQ=255)
                  aGCATTCATCGTGTTGCCCGTATTCATACCCGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTg                                                                                                                               <  2:106569/151‑1 (MQ=255)
                        cATCGTGTTGCCCGTATTCATACCCGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGaa                                                                                                                         <  2:513680/151‑1 (MQ=255)
                           cGTGTTGCCCGTATTCATACCCGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCg                                                                                                                                           <  2:680501/130‑1 (MQ=255)
                           cGTGTTGCCCGTATTCATACCCGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCg                                                                                                                                           >  1:680458/1‑130 (MQ=255)
                                   ccGTATTCATACCCGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTAcgcg                                                                                                              <  2:762183/151‑1 (MQ=255)
                                             aCCCGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAAttt                                                                                                    >  2:496755/1‑151 (MQ=255)
                                              cccGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAAtttt                                                                                                   >  2:810632/1‑151 (MQ=255)
                                               ccGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAAttttt                                                                                                  >  2:194273/1‑151 (MQ=255)
                                               ccGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAAttttt                                                                                                  >  1:462269/1‑151 (MQ=255)
                                                cGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAAtttttt                                                                                                 >  1:717316/1‑151 (MQ=255)
                                                     gACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGtg                                                                                            >  2:342973/1‑151 (MQ=255)
                                                          tGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTGCGTGCTGCa                                                                                       <  2:160775/150‑1 (MQ=255)
                                                           gACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCAt                                                                                      <  1:56666/151‑1 (MQ=255)
                                                            aCAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCt                                                                                                                   <  2:156523/121‑1 (MQ=255)
                                                            aCAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCt                                                                                                                   >  1:156500/1‑121 (MQ=255)
                                                               aCGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCtt                                                                                  <  2:718784/151‑1 (MQ=255)
                                                               aCGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCtt                                                                                  <  1:568872/151‑1 (MQ=255)
                                                                    aCTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATc                                                                             <  1:612737/151‑1 (MQ=255)
                                                                    aCTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATc                                                                             >  2:45092/1‑151 (MQ=255)
                                                                    aCTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATc                                                                             <  2:412133/151‑1 (MQ=255)
                                                                          tAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTaaa                                                                         >  1:492714/1‑149 (MQ=255)
                                                                                ctctctTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCtg                                                                 >  1:33836/1‑151 (MQ=255)
                                                                                    ctTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCtgtg                                                               >  2:819528/1‑149 (MQ=255)
                                                                                      tCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTgaga                                                            >  1:49271/1‑150 (MQ=255)
                                                                                      tCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTgag                                                             <  2:449396/149‑1 (MQ=255)
                                                                                       cGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGaa                                                           <  1:477754/150‑1 (MQ=255)
                                                                                       cGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAAt                                                          <  2:448085/151‑1 (MQ=255)
                                                                                         gACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGt                                                        <  1:267215/151‑1 (MQ=255)
                                                                                             gTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGtt                                                    <  2:189501/151‑1 (MQ=255)
                                                                                                gCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTg                                                  <  1:850700/150‑1 (MQ=255)
                                                                                                 ccGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGct                                                >  2:685527/1‑151 (MQ=255)
                                                                                                 ccGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGct                                                >  2:386952/1‑151 (MQ=255)
                                                                                                 ccGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGct                                                >  2:208583/1‑151 (MQ=255)
                                                                                                  cGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGctc                                               <  2:540113/151‑1 (MQ=255)
                                                                                                  cGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGctc                                               <  1:172072/151‑1 (MQ=255)
                                                                                                   gAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGctct                                              <  2:554060/151‑1 (MQ=255)
                                                                                                   gAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGctct                                              >  2:288187/1‑151 (MQ=255)
                                                                                                    aaaTGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCtt                                             >  1:849407/1‑151 (MQ=255)
                                                                                                    aaaTGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCtt                                             <  2:540674/151‑1 (MQ=255)
                                                                                                        gTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCt                                          <  2:579211/150‑1 (MQ=255)
                                                                                                             cGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTaat                                      <  2:383985/149‑2 (MQ=255)
                                                                                                             cGGAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGtt                                    <  2:376695/151‑1 (MQ=255)
                                                                                                               gAACGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGtttt                                  <  1:215386/151‑1 (MQ=255)
                                                                                                                 aCGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGtttttt                                <  1:498234/151‑1 (MQ=255)
                                                                                                                  cGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTttc                               >  2:35095/1‑151 (MQ=255)
                                                                                                                  cGCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTttc                               >  1:747092/1‑151 (MQ=255)
                                                                                                                   gCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTttct                              <  1:253425/151‑1 (MQ=255)
                                                                                                                   gCATAAATAAAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTttct                              <  2:356879/151‑1 (MQ=255)
                                                                                                                     ataaataaAATAAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTttcttc                            <  2:525084/151‑1 (MQ=255)
                                                                                                                       aaataaaataAGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCa                           <  2:62795/150‑1 (MQ=255)
                                                                                                                        aataaaataaGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAAc                         <  2:442693/151‑1 (MQ=255)
                                                                                                                         ataaaataaGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACa                        <  2:626740/151‑1 (MQ=255)
                                                                                                                           aaaataaGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAg                       <  2:18556/150‑1 (MQ=255)
                                                                                                                            aaataaGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGc                     >  1:335612/1‑151 (MQ=255)
                                                                                                                              ataaGATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCtt                   >  1:176905/1‑151 (MQ=255)
                                                                                                                                  gATGTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCTTTCa                >  2:468796/1‑150 (MQ=255)
                                                                                                                                     gTACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCTTTCACTg             >  1:548836/1‑150 (MQ=255)
                                                                                                                                      tACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCtt                   <  2:602640/143‑1 (MQ=255)
                                                                                                                                      tACTGGCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCtt                   >  1:602600/1‑143 (MQ=255)
                                                                                                                                           gCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCTTTCACTGATGTAc       >  1:928/1‑150 (MQ=255)
                                                                                                                                           gCATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCTTTCACTGATGTACa      <  2:134969/151‑1 (MQ=255)
                                                                                                                                            cATAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCTTTCACTGATGTAc       >  1:473207/1‑149 (MQ=255)
                                                                                                                                             aTAACCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCTTTCACTGATGTACAgg    <  1:618081/151‑1 (MQ=255)
                                                                                                                                               aaCCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCTTTCACTGATGTACAGGc   >  1:846774/1‑150 (MQ=255)
                                                                                                                                               aaCCGCATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCTTTCACTGATGTACAGGCa  <  2:712961/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AAATAATCAAATAGCTAAAGCATTCATCGTGTTGCCCGTATTCATACCCGTGTGACTTTGACAACGGTACTCCTTAGAAACTCTCTTCGGACTGTTGCCGAAATGTTTGCGGAACGCATAAATAAAATAAGATGTACTGGCATAACCACATTGTTCGGCAATTTTATTGACTGAACCTTCTACGCGTATCAAATTTTTTGCGTGCTGCATTCTTGCATCTAAAAGAATCTGTGAGAATGTCGTTTGCTCTTGCTTAAGTTTTTTCTTCAACAGGCTTTCACTGATGTACAGGCA  >  NC_000913/1582920‑1583213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: