Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,586,995 C→T G247D (GGT→GAT)  ydeQ ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,586,9950CT98.1% 191.7 / NA 52G247D (GGT→GAT) ydeQputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (26/25);  minor base A (0/1);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACACCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCC  >  NC_000913/1586849‑1587134
                                                                                                                                                  |                                                                                                                                           
ctgactgGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCAc                                                                                                                                         >  2:713752/1‑151 (MQ=255)
 tgactgGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACt                                                                                                                                        >  2:123105/1‑151 (MQ=255)
    ctgGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTccc                                                                                                                                     >  2:38576/1‑151 (MQ=255)
      ggACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCg                                                                                                                                    <  2:117469/150‑1 (MQ=255)
               cGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTttgt                                                                                                                          <  2:430524/151‑1 (MQ=255)
               cGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTttg                                                                                                                           >  2:668557/1‑150 (MQ=255)
                   tGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGc                                                                                                                       >  1:220212/1‑150 (MQ=255)
                    gAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGc                                                                                                                       <  2:207243/149‑1 (MQ=255)
                     aaaCCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATc                                                                                                                    >  1:505097/1‑151 (MQ=255)
                           tGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGt                                                                                                                <  2:411337/149‑1 (MQ=255)
                                ccTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCAATACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTgtgt                                                                                                         <  1:401485/151‑1 (MQ=255)
                                   gTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGtt                                                                                                        <  1:534313/149‑1 (MQ=255)
                                       gTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCa                                                                                                    >  1:606378/1‑149 (MQ=255)
                                         ccATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAac                                                                                                 <  2:155810/150‑1 (MQ=255)
                                         ccATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAac                                                                                                 >  2:164500/1‑150 (MQ=255)
                                          cATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAacac                                                                                               >  1:81890/1‑151 (MQ=255)
                                             aGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACtt                                                                                             <  2:678793/150‑1 (MQ=255)
                                             aGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTg                                                                                            >  1:422753/1‑151 (MQ=255)
                                                 tGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCg                                                                                         <  2:631209/150‑1 (MQ=255)
                                                   cgcTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCTAGGGAGACCTTTTCTTCCGCAGCCAGTATTTTTCCATTACGCATTAGACTCACATTCACTCCCGTTACTATTGTTGCCTCAAGTACTTTGTTATCATACAATTGTCGTCt                                                                                      >  1:134654/1‑151 (MQ=255)
                                                        aaaCCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTg                                                                                  <  1:794767/150‑1 (MQ=255)
                                                           cccAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCa                                                                               <  2:204330/150‑1 (MQ=255)
                                                            ccAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAgt                                                                             <  1:123082/151‑1 (MQ=255)
                                                              agagGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAgtgg                                                                           >  1:717515/1‑151 (MQ=255)
                                                                  gTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGc                                                                       >  2:687629/1‑151 (MQ=255)
                                                                  gTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGc                                                                       <  2:299335/151‑1 (MQ=255)
                                                                   tACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGc                                                                       >  1:485170/1‑150 (MQ=255)
                                                                       ttGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCAccc                                                                   <  2:81913/150‑1 (MQ=255)
                                                                       ttGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCg                                                                  >  2:246364/1‑151 (MQ=255)
                                                                        tGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCg                                                                  <  1:426286/150‑1 (MQ=255)
                                                                            tttatttaCTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGaa                                                                >  1:196444/1‑148 (MQ=255)
                                                                            tttatttaCTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGaa                                                                >  1:205782/1‑148 (MQ=255)
                                                                                tttaCTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATaa                                                          >  1:302217/1‑150 (MQ=255)
                                                                                 ttaCTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATaaa                                                         >  1:730395/1‑150 (MQ=255)
                                                                                   aCTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAAt                                                       <  2:736778/150‑1 (MQ=255)
                                                                                      gTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATgaga                                                   <  2:545959/151‑1 (MQ=255)
                                                                                          ccAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCtt                                               >  1:805383/1‑151 (MQ=255)
                                                                                           cAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCttt                                              <  1:531182/151‑1 (MQ=255)
                                                                                            aGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCtttt                                             <  2:619143/151‑1 (MQ=255)
                                                                                               gagaCATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACAACCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGAAAACACTTGTCGGCTACTGTCAGTGTTGGCACCCGAAAGATAAAAAGAGAGCtttt                                             <  1:516986/148‑1 (MQ=255)
                                                                                                    aTTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCtt                                     >  2:528757/1‑151 (MQ=255)
                                                                                                    aTTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCtt                                     >  1:140949/1‑151 (MQ=255)
                                                                                                    aTTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCtt                                     >  2:324193/1‑151 (MQ=255)
                                                                                                    aTTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCt                                      <  2:613698/150‑1 (MQ=255)
                                                                                                             cGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAatat                            <  2:703322/151‑1 (MQ=255)
                                                                                                                  ccAGTATTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGcc                       <  1:77988/151‑1 (MQ=255)
                                                                                                                       aTTTTTCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAggg                  >  1:286117/1‑151 (MQ=255)
                                                                                                                          tttCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAgggg                 >  1:834969/1‑149 (MQ=255)
                                                                                                                          tttCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGt                <  1:584682/150‑1 (MQ=255)
                                                                                                                            tCCATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTAt              <  1:397942/150‑1 (MQ=255)
                                                                                                                             ccATTACGCATTAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTAtt             <  2:354555/150‑1 (MQ=255)
                                                                                                                                        tAGAGTGACATCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTcc  >  1:820738/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                           
CTGACTGGACGGTTCCGGCTGAAACCTTGTTTCCTGTTTGTCCATAGGTTGCGCTTAAACCCAGAGGTACTTTGCTTTTATTTACTGTCCCCAGGGAGACATTTTCTCCCGTAGCCAGTATTTTTCCATTACGCATTAGAGTGACACCCACTCCCGATGCTTTTGTTGCATCAGGTGCTGTGTTAGCAAACACTTGTCGGCTACTGTCAGTGGTGGCACCCGAAAGATAAAATGAGAGCTTTTGTTCGCTTGAGCAATATACGCCAAGGGGTATTTCTGCGCTTCC  >  NC_000913/1586849‑1587134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑