| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,609,216 | A→G | intergenic (‑194/+13) | yneE ← / ← uxaB | bestrophin family putative inner membrane protein/altronate oxidoreductase, NAD‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,609,216 | 0 | A | G | 98.2% | 194.9 / ‑4.2 | 54 | intergenic (‑194/+13) | yneE/uxaB | bestrophin family putative inner membrane protein/altronate oxidoreductase, NAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (37/16); total (37/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
CACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAAC > NC_000913/1609071‑1609357 | cacaTTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTaa > 1:196894/1‑150 (MQ=255) acaTTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACg < 2:754021/151‑1 (MQ=255) tcAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGtt > 2:446680/1‑150 (MQ=255) cAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGtt < 2:749528/149‑1 (MQ=255) aaGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGttatt > 2:135994/1‑151 (MQ=255) aCTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACaa < 1:454702/150‑1 (MQ=255) aCAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGAc > 2:207335/1‑151 (MQ=255) cAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGAc < 1:723957/150‑1 (MQ=255) aaaTTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGt > 2:93323/1‑151 (MQ=255) aaGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTcgcg > 1:299140/1‑151 (MQ=255) cTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCAt > 1:243311/1‑151 (MQ=255) aacaTTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCtt > 2:364310/1‑151 (MQ=255) caTTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCtttt > 2:737457/1‑151 (MQ=255) aTTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATGAGCACAACGGACGTACAGCTTCGCGCATCCCttttt < 1:29852/151‑1 (MQ=255) aTGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCa < 1:5869/151‑1 (MQ=255) aCCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCAtt > 1:470152/1‑151 (MQ=255) ttAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCa > 2:7152/1‑150 (MQ=255) aTGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCt > 1:287291/1‑151 (MQ=255) tAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTc > 2:618925/1‑151 (MQ=255) aaaGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCg > 1:367176/1‑151 (MQ=255) aaGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCga < 2:243335/151‑1 (MQ=255) aaGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCg > 2:453300/1‑150 (MQ=255) aGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCgac < 2:549301/151‑1 (MQ=255) atatAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCgaccaga < 1:742499/151‑1 (MQ=255) ataGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCgaccagacc < 1:738386/151‑1 (MQ=255) taGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCgaccagacc > 2:451116/1‑150 (MQ=255) aGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCgaccagacc > 2:120167/1‑149 (MQ=255) gATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGc > 1:350562/1‑151 (MQ=255) gATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGc > 2:631213/1‑151 (MQ=255) aTGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGc > 2:516180/1‑150 (MQ=255) tgttgtAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCAc > 2:809120/1‑151 (MQ=255) gttgtAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCAc < 2:645133/150‑1 (MQ=255) gtAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGc > 1:81375/1‑151 (MQ=255) aaCTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGt > 2:191366/1‑151 (MQ=255) aaCTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGt < 2:47865/151‑1 (MQ=255) aCTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTc > 2:528324/1‑151 (MQ=255) aCTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTc > 2:240079/1‑151 (MQ=255) cTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTc > 1:469805/1‑150 (MQ=255) tatGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTc > 2:257501/1‑151 (MQ=255) gAAATAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTc > 2:510188/1‑151 (MQ=255) tAAGAAACCCGGTCGAAACCGTGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTccc > 1:292588/1‑149 (MQ=255) tAAGAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTccc > 1:364478/1‑149 (MQ=255) gAAACCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTgg > 2:416366/1‑151 (MQ=255) aaCCCGATCGACACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGGACAGCTTAGCGCATCCCTTTATCGAGAATCGCATCCAGGCCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGCGGt > 1:612459/1‑150 (MQ=255) aCCCGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTca < 1:510692/151‑2 (MQ=255) cccGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCtt > 2:738972/1‑151 (MQ=255) cccGGTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCtt > 1:89176/1‑151 (MQ=255) ggTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCttttt < 2:595225/151‑1 (MQ=255) gTCGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTc < 1:135971/151‑1 (MQ=255) cGAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCt < 1:564420/150‑1 (MQ=255) gAAACCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGc > 1:125212/1‑151 (MQ=255) aaCCGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGcc > 2:854451/1‑150 (MQ=255) cGGGTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCaaa > 2:145889/1‑150 (MQ=255) gggTTCAGAGGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGGCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAAc < 2:542775/151‑1 (MQ=255) | CACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAAC > NC_000913/1609071‑1609357 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |