Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,612,510	C→T	G248R (GGG→AGG)	glsB ←	glutaminase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,612,510	0	C	T	100.0%	161.2 / NA	43	G248R (GGG→AGG)	glsB	glutaminase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/22);  total (21/22)

AGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCCCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATATGAATAGCTTTCCCCTGATTAGCCAGAAAGACAAACGTCCGGGCCAGCTCTACACAGC  >  NC_000913/1612365‑1612650                                                                                                                                                  |                                                                                                                                             aGAACGGAAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGACAGCGAGGGCCATTTCATGCGGAACAACCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGt                                                                                                                                         <  1:519793/151‑1 (MQ=255)  gAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGtt                                                                                                                                        >  2:426593/1‑151 (MQ=255)  gAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGtt                                                                                                                                        >  1:261285/1‑151 (MQ=255)        cAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGt                                                                                                                                   >  1:163719/1‑150 (MQ=255)              ccGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATAcc                                                                                                                            >  1:53702/1‑151 (MQ=255)               cGCAAGCGAGTCACCTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATAcc                                                                                                                            >  2:397422/1‑150 (MQ=255)                       aGTTACCGGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCg                                                                                                                    <  1:630437/150‑1 (MQ=255)                            ccTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATc                                                                                                               >  2:76487/1‑150 (MQ=255)                            ccTGCATCATCCAGTTCCGGACGCCAGACAGCGATGGCCATTTCAGGCGGAACAATCGCCAAAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCa                                                                                                              <  2:451276/151‑1 (MQ=255)                                    atcCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTa                                                                                                      <  2:201811/151‑1 (MQ=255)                                         gTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAAttt                                                                                                  >  2:498940/1‑150 (MQ=255)                                              ggACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCcgcg                                                                                            >  2:57553/1‑151 (MQ=255)                                                  tCCAGACATCGGTGGCCACTTCATGCGGAACAATCAACACAATACCCCCACCAACGCCAGATTTCGCCGGAATTCCCACCCGCCAGACTACCTCCCTTTCGTTCTGGTTCATACCACTGGTCCCCATCAGCTCGTTCCTTTGCCGCGCCt                                                                                         >  1:833152/1‑150 (MQ=255)                                                      gACAGCGATGGCCATTTCATGCGGAACAACCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCAt                                                                                     <  1:363955/150‑1 (MQ=255)                                                         aGCGAGGGCCATTTCATGCGGAACAATCGCCACAATCCCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGc                                                                                 <  1:764032/151‑1 (MQ=255)                                                              tGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTcac                                                                            >  2:514058/1‑151 (MQ=255)                                                               ggCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTcacc                                                                           >  1:26051/1‑151 (MQ=255)                                                               ggCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTcacc                                                                           >  2:360051/1‑151 (MQ=255)                                                                 ccATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTcacca                                                                          <  2:507616/150‑1 (MQ=255)                                                                    tttCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTg                                                                       <  2:163742/150‑1 (MQ=255)                                                                       cATGCGGAACAGCCGCCCCAAGCCCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAACCTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTtc                                                                   <  1:250913/151‑1 (MQ=255)                                                                       cATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTtc                                                                   >  1:390983/1‑151 (MQ=255)                                                                              aacaacCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAata                                                            <  1:556093/151‑1 (MQ=255)                                                                                    cGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATATGAATa                                                      <  2:215252/151‑1 (MQ=255)                                                                                     gCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATATGAAt                                                       >  2:175538/1‑149 (MQ=255)                                                                                      ccACAATCCCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATATGAATAg                                                     <  2:20459/150‑1 (MQ=255)                                                                                        acaATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATATGAATAGCtt                                                  >  2:217395/1‑151 (MQ=255)                                                                                           atgccGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATATGAATAGCTTTcc                                               <  1:121056/148‑1 (MQ=255)                                                                                             aCCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATATGAATAGCTTTccc                                              <  1:95529/150‑1 (MQ=255)                                                                                               cGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATATGAATAGCTTTCCCCTg                                           >  2:807410/1‑151 (MQ=255)                                                                                                        cGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATATGAATAGCTTTCCCCTGATTAGCCAg                                  <  2:754995/151‑1 (MQ=255)                                                                                                              aTTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGcc                                                                                               >  1:126420/1‑84 (MQ=255)                                                                                                              aTTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGcc                                                                                               <  2:126443/84‑1 (MQ=255)                                                                                                                  cGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATATGAATAGCTTTCCCCTGATTAGCCAGAAAGACAAAc                        <  2:546205/151‑1 (MQ=255)                                                                                                                  cGCCGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATATGAATAGCTTTCCCCTGATTAGCCAGAAAGACAAAc                        <  1:514022/151‑1 (MQ=255)                                                                                                                     cGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATATGAATAGCTTTCCCCTGATTAGCCAGAAAGACAAACGTc                     >  2:112158/1‑151 (MQ=255)                                                                                                                     cGGTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATATGAATAGCTTTCCCCTGATTAGCCAGAAAGACAAACGTc                     >  2:63115/1‑151 (MQ=255)                                                                                                                      ggTAGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCAATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATATGAATAGCTTTCCCCTGATTAGCCAGAAAGACAAAc                        <  1:438033/147‑1 (MQ=255)                                                                                                                         aGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATATGAATAGCTTTCCCCTGATTAGCCAGAAAGACAAACGTCCggg                 <  2:757657/151‑1 (MQ=255)                                                                                                                         aGCCCCACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATATGAATAGCTTTCCCCTGATTAGCCAGAAAGACAAACGTCCgg                  <  2:87790/150‑1 (MQ=255)                                                                                                                             ccACCCGCCAGGCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATATGAATAGCTTTCCCCTGATTAGCCAGAAAGACAAACGTCCGGGcc               >  1:343836/1‑149 (MQ=255)                                                                                                                                        gCAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCTTGCATTGGCGTCACCACTGGTTCATCAATATGAATAGCTTTCCCCTGATTAGCCAGAAAGACAAACGTCCGGGCCAGCTCTACACAGc  <  2:475306/151‑1 (MQ=255)                                                                                                                                         cAAACTCCCTCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATATGAATAGCTTTCCCCTGATTAGCCAGAAAGACAAACGTCCGGACCAGCTCTACACAGc  >  1:462065/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                             AGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCCCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATATGAATAGCTTTCCCCTGATTAGCCAGAAAGACAAACGTCCGGGCCAGCTCTACACAGC  >  NC_000913/1612365‑1612650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑