Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,618,445	A→G	V155A (GTA→GCA)	marC ←	UPF0056 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,618,445	0	A	G	100.0%	192.0 / NA	52	V155A (GTA→GCA)	marC	UPF0056 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/23);  total (29/23)

ATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGC  >  NC_000913/1618302‑1618592                                                                                                                                                |                                                                                                                                                    atacCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATc                                                                                                                                              >  1:604494/1‑151 (MQ=255)     cagcagGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGCGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCg                                                                                                                                          >  1:83560/1‑151 (MQ=255)     cagcagGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCg                                                                                                                                          <  2:472167/151‑1 (MQ=255)         agGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAgg                                                                                                                                      >  2:440988/1‑151 (MQ=255)         agGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAgg                                                                                                                                      >  1:62440/1‑151 (MQ=255)           gAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGtt                                                                                                                                    >  2:537641/1‑151 (MQ=255)             aTCCCATCAGACGGGAGATCGCTTCAATGCCCCCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGa                                                                                                                                  <  1:715896/151‑1 (MQ=255)              tCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGa                                                                                                                                  >  2:793344/1‑150 (MQ=255)                 cATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAActc                                                                                                                              >  2:672822/1‑151 (MQ=255)                 cATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAActc                                                                                                                              <  1:518255/151‑1 (MQ=255)                     aGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGAc                                                                                                                          >  1:534198/1‑151 (MQ=255)                       aCGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGc                                                                                                                        >  2:582493/1‑151 (MQ=255)                        cGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGc                                                                                                                        >  1:484872/1‑150 (MQ=255)                                 gCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGAc                                                                                                               <  1:533945/150‑1 (MQ=255)                                     cAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCg                                                                                                           >  2:637592/1‑150 (MQ=255)                                       atcccccccTTCCCCACCAGCCGCATACTTGCGCCGGAGCGGCGTAGGCTTCACCCCAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGc                                                                                                             <  1:264781/140‑1 (MQ=255)                                         gCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCt                                                                                                       <  2:244886/150‑1 (MQ=255)                                          ccACCCTTGCCCCCCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCta                                                                                                      <  1:671561/150‑1 (MQ=255)                                             cccTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCtaata                                                                                                   <  2:492618/150‑1 (MQ=255)                                                 tGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAatc                                                                                                <  2:370971/149‑1 (MQ=255)                                                         aGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATgg                                                                                      >  1:31273/1‑151 (MQ=255)                                                          gCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGt                                                                                     <  2:438712/151‑1 (MQ=255)                                                             gCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTcc                                                                                  <  2:566908/151‑1 (MQ=255)                                                                  aTTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGAc                                                                             <  2:735152/151‑1 (MQ=255)                                                                      cgcCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGc                                                                         <  2:806979/151‑1 (MQ=255)                                                                        ccGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGc                                                                         >  2:697739/1‑149 (MQ=255)                                                                              cTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGATGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTg                                                                 >  1:819453/1‑151 (MQ=255)                                                                                       cTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGcc                                                         >  1:227654/1‑150 (MQ=255)                                                                                          ccccACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCg                                                     <  1:440954/151‑1 (MQ=255)                                                                                                aaaaTAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACaa                                               <  2:178670/151‑1 (MQ=255)                                                                                                   aTAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAg                                             <  2:544753/150‑1 (MQ=255)                                                                                                      aCCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCa                                          >  1:775581/1‑150 (MQ=255)                                                                                                          ccaccaGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATAtt                                     >  2:484425/1‑151 (MQ=255)                                                                                                           caccaGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTg                                    <  1:769642/151‑1 (MQ=255)                                                                                                            accaGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTgg                                   >  2:762262/1‑151 (MQ=255)                                                                                                             ccaGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGc                                  >  1:693277/1‑151 (MQ=255)                                                                                                              caGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGc                                  >  1:220892/1‑150 (MQ=255)                                                                                                               aGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCa                                 >  1:201626/1‑150 (MQ=255)                                                                                                               aGGAAAAAGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCAc                                <  1:283832/151‑1 (MQ=255)                                                                                                                  aaaaaGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTgg                             >  1:102117/1‑151 (MQ=255)                                                                                                                   aaaaGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTggg                            >  1:326512/1‑151 (MQ=255)                                                                                                                    aaaGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGt                           >  2:787678/1‑151 (MQ=255)                                                                                                                     aaGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGtt                          <  2:31295/151‑1 (MQ=255)                                                                                                                     aaGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGtt                          >  1:319998/1‑151 (MQ=255)                                                                                                                     aaGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGtt                          >  1:222725/1‑151 (MQ=255)                                                                                                                      aGATCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTc                         <  1:477266/151‑1 (MQ=255)                                                                                                                        aTCAGCGGCGGCGCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCAt                       <  1:777666/151‑1 (MQ=255)                                                                                                                               gcggcgCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAg                <  2:175295/151‑1 (MQ=255)                                                                                                                               gcggcgCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAg                <  1:561397/151‑1 (MQ=255)                                                                                                                                 ggcgCAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGc               >  1:529497/1‑150 (MQ=255)                                                                                                                                    gcAACCATCAGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACACCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAATACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGctc             >  2:592233/1‑149 (MQ=255)                                                                                                                                             aGTGCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCCCAAAGGCAATATTGGCACTGGGTGCATCTTCCAGCTCTTCCGACTTGc  >  2:859226/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                                    ATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGC  >  NC_000913/1618302‑1618592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑