Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,621,321	C→T	intergenic (‑184/‑11)	eamA ← / → ydeE	cysteine and O‑acetyl‑L‑serine efflux system/putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,621,321	0	C	T	100.0%	190.3 / NA	52	intergenic (‑184/‑11)	eamA/ydeE	cysteine and O‑acetyl‑L‑serine efflux system/putative transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/21);  total (31/21)

CAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTA  >  NC_000913/1621173‑1621463                                                                                                                                                     |                                                                                                                                               cAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTgg                                                                                                                                              >  2:145536/1‑151 (MQ=255)  aaCTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTgg                                                                                                                                              >  1:636651/1‑150 (MQ=255)     ttAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGgac                                                                                                                                            >  2:439888/1‑149 (MQ=255)     ttAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGgac                                                                                                                                            >  2:584762/1‑149 (MQ=255)           gAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACaa                                                                                                                                      <  2:431394/149‑1 (MQ=255)                     ttCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATcc                                                                                                                          >  2:410243/1‑151 (MQ=255)                      tCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATccc                                                                                                                         >  1:460988/1‑151 (MQ=255)                       cTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCt                                                                                                                        >  2:673940/1‑151 (MQ=255)                          tAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTacg                                                                                                                     >  2:427587/1‑151 (MQ=255)                           aaCAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTacg                                                                                                                     >  2:703418/1‑150 (MQ=255)                              aGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACaa                                                                                                                                      <  1:195092/130‑1 (MQ=255)                              aGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACaa                                                                                                                                      >  2:195115/1‑130 (MQ=255)                                 tGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGctct                                                                                                              >  1:149009/1‑151 (MQ=255)                                 tGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGctct                                                                                                              >  1:226092/1‑151 (MQ=255)                                  gATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGctct                                                                                                              >  2:589736/1‑150 (MQ=255)                                    ttCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTAcc                                                                                                           >  2:665585/1‑151 (MQ=255)                                         tAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAgcg                                                                                                       <  2:464359/150‑1 (MQ=255)                                                 ggTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTg                                                                                              >  1:772622/1‑151 (MQ=255)                                                   ttACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGcc                                                                                            >  1:235751/1‑151 (MQ=255)                                                     acgacgGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCt                                                                                           <  2:510584/150‑1 (MQ=255)                                                      cgacgGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCtcg                                                                                         <  1:509216/151‑1 (MQ=255)                                                          ggCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTcgtcgt                                                                                     <  2:149032/151‑1 (MQ=255)                                                            cATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCgttg                                                                                   >  2:216872/1‑151 (MQ=255)                                                                aaTGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGttat                                                                               >  2:71250/1‑151 (MQ=255)                                                                aaTGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGttat                                                                               >  2:739633/1‑151 (MQ=255)                                                                 aTGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGttatt                                                                              >  1:58365/1‑151 (MQ=255)                                                                        aaTAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATc                                                                       <  1:560781/151‑1 (MQ=255)                                                                        aaTAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACAATc                                                                       >  1:3920/1‑151 (MQ=255)                                                                            aGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGAc                                                                   <  2:641695/151‑1 (MQ=255)                                                                               cGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGAcgc                                                                 >  2:458810/1‑150 (MQ=255)                                                                               cGCTATACTTCGGATTTTTGCCATGCTACTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGAcgcg                                                                <  2:416940/151‑1 (MQ=255)                                                                                        tCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACg                                                        <  2:636693/150‑1 (MQ=255)                                                                                         cGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCt                                                      <  2:700845/151‑1 (MQ=255)                                                                                                       gCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGAcc                                         <  1:697083/150‑1 (MQ=255)                                                                                                          aTTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCAt                                       <  1:799351/149‑1 (MQ=255)                                                                                                          aTTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCAt                                       <  2:579161/149‑1 (MQ=255)                                                                                                             tCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCAtt                                      >  1:378637/1‑147 (MQ=255)                                                                                                             tCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCAtt                                      <  2:378661/147‑1 (MQ=255)                                                                                                             tCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTAc                                   <  1:589698/150‑1 (MQ=255)                                                                                                                  aCATCTCTAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGt                             <  2:732728/151‑1 (MQ=255)                                                                                                                        ctAAAACAAAACATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGcc                         >  2:289922/1‑149 (MQ=255)                                                                                                                          aaaacaaaacATAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCa                        <  2:10995/148‑1 (MQ=255)                                                                                                                           aaacaaaacaTAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTAc                    >  2:201160/1‑151 (MQ=255)                                                                                                                            aacaaaacaTAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCTTCGGACGCGGCTCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACa                   <  1:576756/151‑1 (MQ=255)                                                                                                                            aacaaaacaTAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTAc                    >  2:225776/1‑150 (MQ=255)                                                                                                                             acaaaacaTAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTAc                    >  2:594672/1‑149 (MQ=255)                                                                                                                              caaaacaTAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGc                 >  2:681001/1‑151 (MQ=255)                                                                                                                                aaacaTAACGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCt               <  2:190947/151‑1 (MQ=255)                                                                                                                                        cGAAACGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCg       >  2:677359/1‑151 (MQ=255)                                                                                                                                           aaCGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATc    <  1:594633/151‑1 (MQ=255)                                                                                                                                           aaCGCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATc    >  1:486500/1‑151 (MQ=255)                                                                                                                                              gCACTGCTGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTa  >  2:524242/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                               CAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTTTGCCATGCTATTTCTTTACATCTCTAAAACAAAACATAACGAAACGCACTGCCGGACAGACAAATGAACTTATCCCTACGACGCTCTACCAGCGCCCTTCTTGCCTCGTCGTTGTTATTAACCATCGGACGCGGCGCTACGCTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTA  >  NC_000913/1621173‑1621463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑