Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,711,501	A→G	N225S (AAC→AGC)	rsxE →	SoxR iron‑sulfur cluster reduction factor component; electron transport inner membrane NADH‑quinone reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,711,501	0	A	G	100.0%	176.7 / NA	52	N225S (AAC→AGC)	rsxE	SoxR iron‑sulfur cluster reduction factor component; electron transport inner membrane NADH‑quinone reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/21);  total (31/21)

TTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAACGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACTGCTTTCCGCTCAGGCG  >  NC_000913/1711356‑1711648                                                                                                                                                  |                                                                                                                                                    ttCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCgg                                                                                                                                                  <  1:661925/149‑1 (MQ=255) ttCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTg                                                                                                                                                >  2:364211/1‑151 (MQ=255)       acCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACa                                                                                                                                           <  1:437097/150‑1 (MQ=255)         cGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAggg                                                                                                                                        <  1:704368/151‑1 (MQ=255)           aCTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGaa                                                                                                                                      <  2:773230/151‑1 (MQ=255)              ccccTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAAt                                                                                                                                     <  1:488438/149‑1 (MQ=255)                   ttCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGGGAAACAGGGAATGTCtgat                                                                                                                              >  1:474740/1‑151 (MQ=255)                        gctgGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATa                                                                                                                         <  2:289299/151‑1 (MQ=255)                         ctgGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATa                                                                                                                         <  1:752683/150‑1 (MQ=255)                             cGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCa                                                                                                                    <  2:480587/151‑1 (MQ=255)                                     ccaccaGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGc                                                                                                              <  1:852310/149‑1 (MQ=255)                                       accaGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTgg                                                                                                          >  2:765573/1‑151 (MQ=255)                                                  ttATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCAc                                                                                                <  1:715420/150‑1 (MQ=255)                                                         ccTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCt                                                                                          >  2:536106/1‑149 (MQ=255)                                                           tGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGt                                                                                      >  1:833021/1‑151 (MQ=255)                                                               aCTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTg                                                                                     >  1:548636/1‑148 (MQ=255)                                                                 tGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAAc                                                                                >  1:606302/1‑151 (MQ=255)                                                                  gATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAAc                                                                                >  1:674484/1‑150 (MQ=255)                                                                         gCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTTCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCa                                                                        <  2:606796/151‑1 (MQ=255)                                                                            ggAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATcc                                                                     >  2:276933/1‑151 (MQ=255)                                                                            ggAAAATACATGATTGCTGAAAGAATGAAAAAGCGCCGTGCTGAAGTAGCTGCAGAACGTGCATTGCCAAGAGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACGCGCCTGCGTGAGAACAATCCTCATc                                                                      >  1:465784/1‑150 (MQ=255)                                                                              aaaaTACCTGATTGATGAAAGAATGAAAAAGCGCCGGGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATccc                                                                    >  1:621199/1‑150 (MQ=255)                                                                               aaaTACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTATGATGAATAAAGCAAAACGACTGGAGATCCTCACTCGCCTGCGTGAGAACAATCTTCATccaa                                                                   >  1:700776/1‑148 (MQ=255)                                                                                aaTACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCccacc                                                                 >  2:217926/1‑151 (MQ=255)                                                                                aaTACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCccacc                                                                 >  2:107073/1‑151 (MQ=255)                                                                                 aTACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCccacc                                                                 >  2:374664/1‑150 (MQ=255)                                                                                   aCCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCaccacc                                                              <  2:45377/151‑1 (MQ=255)                                                                                     cTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCg                                                             >  1:22168/1‑150 (MQ=255)                                                                                      tgattgatGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCg                                                             >  1:677857/1‑149 (MQ=255)                                                                                      tgattgatGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCg                                                             >  2:57277/1‑149 (MQ=255)                                                                                       gattgatGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGc                                                          >  2:115540/1‑151 (MQ=255)                                                                                            atgaAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCAGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAAt                                                     >  2:339361/1‑151 (MQ=255)                                                                                                  gAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGt                                               >  2:518056/1‑151 (MQ=255)                                                                                                      gAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGc                                           >  2:780000/1‑151 (MQ=255)                                                                                                        aaaaGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCt                                         >  1:815480/1‑151 (MQ=255)                                                                                                          aaGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCttt                                       <  1:34268/151‑1 (MQ=255)                                                                                                             cgcCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGa                                    <  1:276908/151‑1 (MQ=255)                                                                                                              gcCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGa                                    >  2:365216/1‑150 (MQ=255)                                                                                                              gcCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGa                                    >  2:601526/1‑150 (MQ=255)                                                                                                               ccGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAAt                                  >  2:318037/1‑151 (MQ=255)                                                                                                               ccGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAAt                                  <  2:378808/151‑1 (MQ=255)                                                                                                                cGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAAtt                                 <  1:364188/151‑1 (MQ=255)                                                                                                                     tGAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTg                             >  2:776082/1‑150 (MQ=255)                                                                                                                      gAAGCAGCTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGa                            <  1:746273/150‑1 (MQ=255)                                                                                                                         gcagcTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGc                        >  2:792730/1‑151 (MQ=255)                                                                                                                          cagcTGCAGAACGTGCATTGCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGc                        >  1:489582/1‑150 (MQ=255)                                                                                                                                      gcgCATTGCCAAGCGGTGAAACGGGGAATGTCTGCTGAAAAAAGCAAAACGCCTAGAGGTCCTCACTAGCCGGCGTGAGAACAATCTTCATCCCACCACCGTGGTTGATGTCAGTTCGCCTTTTGAATTGCTGATTGCCGTCCTGCTTTc            <  1:102818/148‑1 (MQ=255)                                                                                                                                             gCCAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACTGCTTTCCGCTCAgg    <  1:739783/151‑1 (MQ=255)                                                                                                                                              ccAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACTGCTTTCCGCTCAgg    >  1:118440/1‑150 (MQ=255)                                                                                                                                              ccAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACTGCTTTCCGCTCAGGc   <  1:144547/151‑1 (MQ=255)                                                                                                                                               cAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAGAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTAAGTTCGCCTTTTGAATTGCTGATTGCCGTACTGCTTTCCGCTCAgg    >  1:841811/1‑149 (MQ=255)                                                                                                                                               cAAGCGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACTGCTTTCCGCTCAGGCg  <  2:674526/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    TTCCACACCGACTCCCCTTTCCTGCTGGCGATGCTGCCACCAGGTGCATTTATTGGCCTGGGACTGATGCTGGCAGGAAAATACCTGATTGATGAAAGAATGAAAAAGCGCCGTGCTGAAGCAGCTGCAGAACGTGCATTGCCAAACGGTGAAACAGGGAATGTCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACTGCTTTCCGCTCAGGCG  >  NC_000913/1711356‑1711648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑