Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,751,240	T→C	E162G (GAA→GGA)	ydhW ←	FNR, Nar, NarP‑regulated protein; putative subunit of YdhYVWXUT oxidoreductase complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,751,240	0	T	C	100.0%	187.7 / NA	53	E162G (GAA→GGA)	ydhW	FNR, Nar, NarP‑regulated protein; putative subunit of YdhYVWXUT oxidoreductase complex
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/22);  total (31/22)

AACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCAAAGACATCGCCGC  >  NC_000913/1751090‑1751385                                                                                                                                                       |                                                                                                                                                  aaCTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCtt                                                                                                                                                   >  2:762331/1‑151 (MQ=255)      ctctACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATc                                                                                                                                              >  1:149914/1‑151 (MQ=255)       tctACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCt                                                                                                                                             >  1:19825/1‑151 (MQ=255)             tCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCtt                                                                                                                                       >  2:507409/1‑151 (MQ=255)                aaCCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCt                                                                                                                                    >  2:780864/1‑151 (MQ=255)                aaCCATTCGCATAAGCCATAAGCCTTGCCATAGGTCAAAAATCGCGCGCTAAACAAGTAAACCACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCt                                                                                                                                        <  1:469923/147‑1 (MQ=255)                   cATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGa                                                                                                                                 <  2:46101/151‑1 (MQ=255)                     ttCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGa                                                                                                                                 >  2:268950/1‑149 (MQ=255)                      tCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGa                                                                                                                                 >  1:662921/1‑148 (MQ=255)                         cataagccataagcCTTGCCATAGGTCATAAACCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAat                                                                                                                           <  2:22062/151‑1 (MQ=255)                          ataagccataagccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAata                                                                                                                          <  1:321885/151‑1 (MQ=255)                          ataagccataagccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAat                                                                                                                           <  2:491565/150‑1 (MQ=255)                          ataagccataagccTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAat                                                                                                                           >  1:491529/1‑150 (MQ=255)                                          gCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGc                                                                                                          >  2:424864/1‑151 (MQ=255)                                           ccATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGc                                                                                                          >  2:96371/1‑150 (MQ=255)                                              tAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATgg                                                                                                      >  1:630706/1‑151 (MQ=255)                                               aGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGc                                                                                                     >  2:472983/1‑151 (MQ=255)                                                ggTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGc                                                                                                     <  1:402926/150‑1 (MQ=255)                                                ggTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGc                                                                                                     <  2:816860/150‑1 (MQ=255)                                                     tAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAAAATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACtt                                                                                                <  2:812007/150‑1 (MQ=255)                                                            ctcgctAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAgg                                                                                         >  2:403011/1‑150 (MQ=255)                                                            ctcgctAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAgg                                                                                         >  1:389305/1‑150 (MQ=255)                                                             tcgctAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGAc                                                                                       >  2:701452/1‑151 (MQ=255)                                                              cgctAAACAGGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGa                                                                                        <  2:839849/149‑1 (MQ=255)                                                               gctAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGa                                                                                     <  1:454085/151‑1 (MQ=255)                                                                  aaaCAAGTACAACACCGCCGTCGATGACTCCCCCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAgg                                                                                   <  1:257799/150‑1 (MQ=255)                                                                   aaCAAGTACAACCCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGAt                                                                                 <  1:141173/151‑1 (MQ=255)                                                                    aCAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGAt                                                                                 >  2:395083/1‑150 (MQ=255)                                                                      aagaccagcacCGCCATCGATGCCTCCACCTGCCGTATATCTGCATATTAAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCATCATGGCGACTTAGTAATGACGAGGATACg                                                                              <  1:334597/143‑1 (MQ=255)                                                                      aaGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACg                                                                              <  1:394791/151‑1 (MQ=255)                                                                         tACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTc                                                                            <  2:230380/150‑1 (MQ=255)                                                                         tACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCt                                                                           >  2:135322/1‑151 (MQ=255)                                                                           caacaCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTgg                                                                         >  1:308963/1‑151 (MQ=255)                                                                           caacaCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGCGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTg                                                                          >  1:697472/1‑150 (MQ=255)                                                                            aacaCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGc                                                                        >  2:493342/1‑151 (MQ=255)                                                                            aacaCCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGc                                                                        <  2:100832/151‑1 (MQ=255)                                                                                     gTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAaac                                                               >  2:544292/1‑151 (MQ=255)                                                                                         aTGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAaacgaa                                                            <  2:653598/150‑1 (MQ=255)                                                                                           gACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACgg                                                         >  1:633154/1‑151 (MQ=255)                                                                                                   cTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGc                                                  >  1:30849/1‑150 (MQ=255)                                                                                                     gCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAAt                                               >  2:568633/1‑151 (MQ=255)                                                                                                      ccGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATg                                              <  1:301075/151‑1 (MQ=255)                                                                                                      ccGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATg                                              >  1:77460/1‑151 (MQ=255)                                                                                                            atCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATAAGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGcgcg                                         <  2:297598/150‑1 (MQ=255)                                                                                                                     ttCAGGTGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACAAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTcc                               >  2:455675/1‑151 (MQ=255)                                                                                                                           tGCAACATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTcc                         >  2:416361/1‑151 (MQ=255)                                                                                                                              aaCATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCcac                       >  2:150620/1‑150 (MQ=255)                                                                                                                               aCATCCATCGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACaa                     <  1:686421/151‑1 (MQ=255)                                                                                                                                 atccatcGCGGCGATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACt                   <  2:29862/151‑1 (MQ=255)                                                                                                                                         cggcgATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCaa              >  2:614932/1‑148 (MQ=255)                                                                                                                                            cgATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCAAAGACAt        <  1:363425/151‑1 (MQ=255)                                                                                                                                             gATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCAAAGACATc       >  2:175166/1‑151 (MQ=255)                                                                                                                                             gATCGCGGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCAAAGACATc       >  2:296233/1‑151 (MQ=255)                                                                                                                                                  cgGCTCCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCAAAGACATcgccgc  >  2:668933/1‑151 (MQ=255)                                                                                                                                                       |                                                                                                                                                  AACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGCTCCACAACTTGCAAAGACATCGCCGC  >  NC_000913/1751090‑1751385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑