Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,781,206	G→A	G269D (GGC→GAC)	ydiR →	putative electron transfer flavoprotein, FAD‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,781,206	0	G	A	100.0%	165.9 / NA	45	G269D (GGC→GAC)	ydiR	putative electron transfer flavoprotein, FAD‑binding subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/17);  total (28/17)

CTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGGATGGAGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACC  >  NC_000913/1781065‑1781353                                                                                                                                              |                                                                                                                                                    ctgaaGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGGATGGAGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACgg                                                                                                                                            >  2:466157/1‑151 (MQ=255)    aaGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGGATGGAGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGgcgc                                                                                                                                         >  2:768970/1‑151 (MQ=255)      gTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGGATGGAGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCa                                                                                                                                        >  1:65108/1‑150 (MQ=255)             gTTCACGTCCAATTGCCGAAGGCGAGAACTGGATGGAGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGAt                                                                                                                                <  2:40978/151‑1 (MQ=255)              ttCACGTCCAATTGCCGAAGGCGAGAACTGGATGGAGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGAtt                                                                                                                               >  1:650141/1‑151 (MQ=255)                cccggccAAATGCCGAAGGCGAGAACTGGATGGAGCGTGGACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCTTATGGTTGACGGCAACGGCGCAAAAGTTATTg                                                                                                                              <  1:605101/145‑1 (MQ=38)                   gTCCAATTGCCGAAGGCGAGAACTGGATGGAGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGc                                                                                                                          >  1:561148/1‑151 (MQ=255)                     ccAATTGCCGAAGGCGAGAGCTGGATGGAGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGcct                                                                                                                        <  1:78144/151‑2 (MQ=255)                        aTTGCCGAAGGCGAGAACTGGATGGAGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCa                                                                                                                     <  2:602338/151‑1 (MQ=255)                          tGCCGAAGGCGAGAACTGGATGGAGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCaa                                                                                                                    <  1:733490/150‑1 (MQ=255)                             cGAAGGCGAGAACTGGATGGAGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATaa                                                                                                                 >  2:716188/1‑150 (MQ=255)                                aGGCGAGAACTGGATGGAGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATaa                                                                                                                 >  1:362711/1‑147 (MQ=255)                                      gaACTGGATGGAGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAAt                                                                                                       <  1:171083/151‑1 (MQ=255)                                      gaACTGGATGGAGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAAt                                                                                                       <  2:141242/151‑1 (MQ=255)                                       aaCTGGATGGAGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATg                                                                                                      <  1:278344/151‑1 (MQ=255)                                          tggatggaGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATgcgc                                                                                                   >  2:546464/1‑151 (MQ=255)                                          tggatggaGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATgcgc                                                                                                   >  1:840911/1‑151 (MQ=255)                                              tggaGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAAt                                                                                               >  2:449937/1‑151 (MQ=255)                                                gaGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCt                                                                                             <  1:357201/151‑1 (MQ=255)                                                    gTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCaa                                                                                         <  1:662615/151‑1 (MQ=255)                                                       aaCGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACt                                                                                       >  2:156827/1‑150 (MQ=255)                                                       aaCGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTa                                                                                      <  1:406001/151‑1 (MQ=255)                                                       aaCGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTa                                                                                      <  2:650183/151‑1 (MQ=255)                                                          gTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGc                                                                                   >  2:649390/1‑151 (MQ=255)                                                           ttATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGcc                                                                                  >  1:173727/1‑151 (MQ=255)                                                                tCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACt                                                                              >  2:107666/1‑150 (MQ=255)                                                                    tgtCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGt                                                                         >  1:855538/1‑151 (MQ=255)                                                                      tctcCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCt                                                                       >  1:829438/1‑151 (MQ=255)                                                                           ggCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCtggtgg                                                                  >  1:707177/1‑151 (MQ=255)                                                                              gtgtTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCg                                                               >  1:713265/1‑151 (MQ=255)                                                                                gtTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGat                                                             >  2:121722/1‑151 (MQ=255)                                                                                gtTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGa                                                              <  1:812639/150‑1 (MQ=255)                                                                                  tGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGatat                                                           >  2:162627/1‑151 (MQ=255)                                                                                        aaTCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACaa                                                     <  2:819383/151‑1 (MQ=255)                                                                                                ctctACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTcc                                             >  1:709709/1‑151 (MQ=255)                                                                                                 tctACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTccc                                            >  1:257634/1‑151 (MQ=255)                                                                                                    aCCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTccctgc                                         >  2:629514/1‑151 (MQ=255)                                                                                                               gggATCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTCCCTGCCCTGATTATcc                              <  1:767023/151‑1 (MQ=255)                                                                                                                  aTCTCCGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGTTAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGt                           >  1:108538/1‑151 (MQ=255)                                                                                                                      ccGGGCAGATCCAGCATATTGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAg                            <  1:474549/146‑1 (MQ=255)                                                                                                                      ccGGGCAGATCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTg                         >  2:226791/1‑149 (MQ=255)                                                                                                                               tCCAGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAAtt              >  1:690750/1‑151 (MQ=255)                                                                                                                                  aGCATATGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTccc           >  1:69095/1‑151 (MQ=255)                                                                                                                                       atGGTTGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTc      <  2:815496/151‑1 (MQ=255)                                                                                                                                           ttGACGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAGcc  >  2:798900/1‑151 (MQ=255)                                                                                                                                              |                                                                                                                                                    CTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGGATGGAGCGTGAACGTTATATCGGTGTCTCCGGCGTGTTGCTGAAATCCGATCTCTACCTGACGCTGGGGATCTCCGGGCAGATCCAGCATATGGTTGGCGGCAACGGCGCAAAAGTGATTGTCGCCATCAATAAAGATAAAAATGCGCCAATCTTCAACTATGCCGACTACGGTCTGGTGGGCGATATCTACAAAGTCGTCCCTGCCCTGATTAGCCAGTTGAGCCGCTAATTCCCCTTTCAACC  >  NC_000913/1781065‑1781353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑