Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,821,936	A→T	D107E (GAT→GAA)	osmE ←	osmotically‑inducible lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,821,936	0	A	T	100.0%	241.6 / NA	66	D107E (GAT→GAA)	osmE	osmotically‑inducible lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (36/30);  total (36/30)

CTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACT  >  NC_000913/1821789‑1822083                                                                                                                                                     |                                                                                                                                                    cTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTc                                                                                                                                                   >  2:324837/1‑150 (MQ=255)   tGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAgt                                                                                                                                                >  1:541577/1‑151 (MQ=255)   tGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAgt                                                                                                                                                >  1:156853/1‑151 (MQ=255)     aCCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAgtgt                                                                                                                                              <  2:359599/151‑1 (MQ=255)           ggTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTAtt                                                                                                                                        <  2:32284/151‑1 (MQ=255)           ggTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTAt                                                                                                                                         <  2:402574/150‑1 (MQ=255)               aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGc                                                                                                                                    <  2:307790/151‑1 (MQ=255)                 gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGc                                                                                                                                    >  1:493311/1‑150 (MQ=255)                   cccTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAg                                                                                                                                <  2:150756/151‑1 (MQ=255)                      tctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAAACCAGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTc                                                                                                                             >  2:186803/1‑151 (MQ=255)                         tCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCt                                                                                                                            <  2:446122/149‑1 (MQ=255)                          cacaTTACGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTc                                                                                                                             <  2:67549/147‑1 (MQ=255)                            caTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATa                                                                                                                        >  2:806477/1‑150 (MQ=255)                                tCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCgg                                                                                                                   >  1:198558/1‑151 (MQ=255)                                 cGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGa                                                                                                                  <  1:472707/151‑1 (MQ=255)                                  gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑CAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGTTCTGATAACCGGAg                                                                                                                 <  1:615717/151‑1 (MQ=255)                                      aaaTAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTtga                                                                                                             <  1:218881/151‑1 (MQ=255)                                        ataatTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTtgatg                                                                                                           >  1:11141/1‑151 (MQ=255)                                        ataatTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTtgatg                                                                                                           >  1:845493/1‑151 (MQ=255)                                           atTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAc                                                                                                         >  1:111858/1‑150 (MQ=255)                                           atTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAc                                                                                                         >  1:533890/1‑150 (MQ=255)                                           atTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACa                                                                                                        <  1:745907/151‑1 (MQ=255)                                           atTCGCGAAGATAATTAAGAACTCAGGCC‑‑AAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACAt                                                                                                       <  2:766426/151‑1 (MQ=255)                                              cgcgAAGATAATTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGt                                                                                                     >  2:269351/1‑151 (MQ=255)                                               gcgAAGATAATTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTc                                                                                                    >  1:76163/1‑151 (MQ=255)                                                 gAAGATAATTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCg                                                                                                  <  1:675737/151‑1 (MQ=255)                                                  aaGATAATTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCgg                                                                                                 >  1:44566/1‑151 (MQ=255)                                                     aTAATTCAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTat                                                                                              >  1:599169/1‑151 (MQ=255)                                                       aaTTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTatca                                                                                            <  1:363109/151‑1 (MQ=255)                                                       aaTTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTatc                                                                                             >  1:752552/1‑150 (MQ=255)                                                        aTTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTatcat                                                                                           <  1:40147/151‑1 (MQ=255)                                                        aTTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTatcat                                                                                           >  1:91204/1‑151 (MQ=255)                                                          tAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCt                                                                                         >  2:650441/1‑151 (MQ=255)                                                           aaGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGTCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCt                                                                                         >  2:747439/1‑150 (MQ=255)                                                           aaGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCt                                                                                         >  2:270246/1‑150 (MQ=255)                                                              agcTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAAcg                                                                                     <  1:737360/149‑1 (MQ=255)                                                              aaCTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAAcg                                                                                     >  2:170103/1‑151 (MQ=255)                                                                    ggCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACa                                                                                >  1:574475/1‑150 (MQ=255)                                                                     gCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACaa                                                                               >  2:16566/1‑150 (MQ=255)                                                                       c‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGt                                                                            >  1:761605/1‑151 (MQ=255)                                                                           aaaaaaCCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAgg                                                                         <  1:68337/151‑1 (MQ=255)                                                                              aaaCCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGttt                                                                      >  1:43660/1‑151 (MQ=255)                                                                              aaaCCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGttt                                                                      <  1:192429/151‑1 (MQ=255)                                                                               aaCCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTc                                                                     >  2:458734/1‑151 (MQ=255)                                                                                   ggCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCt                                                                 >  1:335295/1‑151 (MQ=255)                                                                                           ggCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATc                                                         <  2:135635/151‑1 (MQ=255)                                                                                                gTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGtt                                                    >  2:802168/1‑151 (MQ=255)                                                                                                   tCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGa                                                  <  2:316927/150‑1 (MQ=255)                                                                                                     cGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGAccc                                               >  2:40494/1‑151 (MQ=255)                                                                                                          ttACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAt                                          >  1:686689/1‑151 (MQ=255)                                                                                                           tACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGAt                                          <  1:16545/150‑1 (MQ=255)                                                                                                                 aGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTc                                   <  1:517585/151‑1 (MQ=255)                                                                                                                 aGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTc                                   <  2:430896/151‑1 (MQ=255)                                                                                                                     ttCAGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTgg                                <  2:783122/150‑1 (MQ=255)                                                                                                                        aGACGAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAg                            <  1:186780/151‑1 (MQ=255)                                                                                                                            gAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTAcc                        >  1:325603/1‑151 (MQ=255)                                                                                                                            gAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTAcc                        >  1:480970/1‑151 (MQ=255)                                                                                                                            gAATTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTAcc                        <  1:710343/151‑1 (MQ=255)                                                                                                                             aaTTGATTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTAcc                        >  2:544284/1‑150 (MQ=255)                                                                                                                                  aTTACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGc                  <  2:394131/151‑1 (MQ=255)                                                                                                                                   ttACTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGc                  >  2:182070/1‑150 (MQ=255)                                                                                                                                     aCTTCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATg               >  2:734951/1‑151 (MQ=255)                                                                                                                                        tCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGa             <  2:601247/150‑1 (MQ=255)                                                                                                                                        tCGCAGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGa             <  2:714056/150‑1 (MQ=255)                                                                                                                                            aGCCTGTGGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATg        <  2:493347/151‑1 (MQ=255)                                                                                                                                                  tgGTTCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACt  >  1:211774/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑AAAAAAAACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCATCTAACGCGACAAAGTAGGTTTCTGCTTTACCATCACGTTGACCCAGGATGTAGGTCTGGCAAGTACCGCGAGCATGGATCATGCTCACT  >  NC_000913/1821789‑1822083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑