Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,824,020	T→C	L169P (CTG→CCG)	cho →	endonuclease of nucleotide excision repair

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,824,020	0	T	C	100.0%	173.0 / NA	49	L169P (CTG→CCG)	cho	endonuclease of nucleotide excision repair
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/27);  total (22/27)

AATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCTTGCGCCAGTCTCTGGAGCGTTTGCGGGTGGTG  >  NC_000913/1823873‑1824162                                                                                                                                                    |                                                                                                                                               aaTTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCccgct                                                                                                                                             >  1:71283/1‑151 (MQ=255) aaTTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCccgct                                                                                                                                             <  2:275843/151‑1 (MQ=255)        tGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTg                                                                                                                                      >  1:533059/1‑151 (MQ=255)         gAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTgg                                                                                                                                     >  2:708629/1‑151 (MQ=255)           aaaGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGa                                                                                                                                    >  2:752077/1‑150 (MQ=255)               cgcgTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGt                                                                                                                               <  1:708585/151‑1 (MQ=255)                 cgTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGtt                                                                                                                              >  1:273724/1‑150 (MQ=255)                     gATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTc                                                                                                                         <  2:68602/151‑1 (MQ=255)                      aTGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCGTCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTcg                                                                                                                        <  2:516125/151‑1 (MQ=255)                        gtggtgTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTcgc                                                                                                                       <  1:415244/150‑1 (MQ=255)                             gtATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGt                                                                                                                 <  2:340077/151‑1 (MQ=255)                             gtATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATCGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCg                                                                                                                  <  2:645917/150‑1 (MQ=255)                               aTGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGc                                                                                                               >  2:270915/1‑151 (MQ=255)                                  ccAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATg                                                                                                            <  2:265842/151‑1 (MQ=255)                                       gagGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTc                                                                                                       <  2:779180/151‑1 (MQ=255)                                       gagGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTc                                                                                                       <  2:375150/151‑1 (MQ=255)                                        agGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCg                                                                                                      >  1:177374/1‑151 (MQ=255)                                        agGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCg                                                                                                      >  2:738037/1‑151 (MQ=255)                                         ggTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCg                                                                                                      <  1:579579/150‑1 (MQ=255)                                                    cACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTaaa                                                                                          <  2:684137/151‑1 (MQ=255)                                                    cACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTaaa                                                                                          <  2:270996/151‑1 (MQ=255)                                                      cGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAAc                                                                                        >  2:205742/1‑151 (MQ=255)                                                       gAGCCCCCACCCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACg                                                                                       <  1:44814/151‑1 (MQ=255)                                                       gAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACg                                                                                       >  2:311385/1‑151 (MQ=255)                                                         gCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGtt                                                                                     <  2:252388/151‑1 (MQ=255)                                                              cAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGcc                                                                                >  1:500185/1‑151 (MQ=255)                                                               aaCCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCg                                                                               >  2:791874/1‑151 (MQ=255)                                                                  cTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCgg                                                                              >  2:523630/1‑149 (MQ=255)                                                                    gTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGc                                                                           >  2:271748/1‑150 (MQ=255)                                                                      ttGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCAt                                                                         <  1:442803/150‑1 (MQ=255)                                                                        ggCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCAtgc                                                                       <  2:692798/150‑1 (MQ=255)                                                                          ccTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCAtgctgc                                                                    <  2:715984/151‑1 (MQ=255)                                                                               ttGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTa                                                                <  1:53875/150‑1 (MQ=255)                                                                                 gCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAag                                                             <  1:791818/151‑1 (MQ=255)                                                                                          cgcgcAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATg                                                    <  2:575961/151‑1 (MQ=255)                                                                                            cgcAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATg                                                    >  2:799028/1‑149 (MQ=255)                                                                                             gcAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAgg                                                 >  1:580755/1‑151 (MQ=255)                                                                                              cAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAgg                                                 >  2:575187/1‑150 (MQ=255)                                                                                               aGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGa                                                >  2:421303/1‑150 (MQ=255)                                                                                                    tGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATg                                           <  2:273749/150‑1 (MQ=255)                                                                                                                aGACCATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCTTgcg                              <  1:785354/151‑1 (MQ=255)                                                                                                                   ccATCGCTGATGAACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCTTGCGcc                            >  2:501736/1‑150 (MQ=255)                                                                                                                          tgatgaACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCTTGCGCCAGTCTCTg                    >  2:560942/1‑151 (MQ=255)                                                                                                                           gatgaACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCTTGCGCCAGTCTCTgg                   <  2:606259/151‑1 (MQ=255)                                                                                                                            atgaACAAAAACTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCTTGCGCCAGTCTCTGGa                  <  1:162675/151‑1 (MQ=255)                                                                                                                                    aaaCTTTGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCTTGCGCCAGTCTCTGGAGCGTTTGc          <  1:553457/151‑1 (MQ=255)                                                                                                                                          tGTTATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCTTGCGCCAGTCTCTGGAGCGTTTGCGggtgg    >  1:615932/1‑151 (MQ=255)                                                                                                                                            ttATGGCCCGCTGGGTCTCGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCTTGCGCCAGTCTCTGGAGCGTTTGCGggtggt   <  2:208649/150‑1 (MQ=255)                                                                                                                                            ttATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCTTGCGCCAGTCTCTGGAGCGTTTGCGGgtggtg  >  1:686103/1‑151 (MQ=255)                                                                                                                                             tATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCTTGCGCCAGTCTCTGGAGCGTTTGCGGgtggtg  >  2:311912/1‑150 (MQ=255)                                                                                                                                             tATGGCCCGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCTTGCGCCAGTCTCTGGAGCGTTTGCGGgtggtg  <  2:84740/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                               AATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCATTGCAGACCATCGCTGATGAACAAAAACTTTGTTATGGCCTGCTGGGACTGGAACCGTTAAGTCGCGGTCGTGCATGTTTTCGTTCAGCGCTAAAACGTTGCGCCGGAGCATGCTGCGGTAAAGAGAGCCATGAGGAACATGCGCTACGCTTGCGCCAGTCTCTGGAGCGTTTGCGGGTGGTG  >  NC_000913/1823873‑1824162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑