Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,829,705	T→C	I10M (ATA→ATG)	astD ←	succinylglutamic semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,829,705	0	T	C	100.0%	132.4 / NA	37	I10M (ATA→ATG)	astD	succinylglutamic semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/19);  total (18/19)

AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACG  >  NC_000913/1829566‑1829850                                                                                                                                            |                                                                                                                                                  aaaGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCAcc                                                                                                                                        <  2:244842/151‑1 (MQ=255) aaaGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCAcc                                                                                                                                        <  1:536565/151‑1 (MQ=255) aaaGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCAcc                                                                                                                                        >  1:601411/1‑151 (MQ=255)     ccGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGtt                                                                                                                                     >  2:138508/1‑150 (MQ=255)            cAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATa                                                                                                                             <  1:414882/151‑1 (MQ=255)                gcgGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGt                                                                                                                         <  1:128646/151‑1 (MQ=255)                 cgGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTc                                                                                                                        <  2:286165/151‑1 (MQ=255)                     aaCGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGc                                                                                                                    >  2:477566/1‑151 (MQ=255)                           gcACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGtttt                                                                                                              <  2:773763/151‑1 (MQ=255)                           gcACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGtttt                                                                                                              <  2:73904/151‑1 (MQ=255)                                    gCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTgc                                                                                                     >  2:769884/1‑151 (MQ=255)                                     cccGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTgcg                                                                                                    <  1:315006/151‑1 (MQ=255)                                                    cGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGa                                                                                     <  2:11910/151‑1 (MQ=255)                                                      aCCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACACGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACg                                                                                   <  1:751004/151‑1 (MQ=255)                                                               gcatcggcatcATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATc                                                                          <  2:178364/151‑1 (MQ=255)                                                                         catTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTgg                                                                <  1:71528/151‑1 (MQ=255)                                                                         catTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTgg                                                                <  2:553436/151‑1 (MQ=255)                                                                            tgccttgccATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCAt                                                             >  2:410727/1‑151 (MQ=255)                                                                               cttgccATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTg                                                          >  2:273645/1‑151 (MQ=255)                                                                                     aTAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGc                                                     >  2:313661/1‑150 (MQ=255)                                                                                              cGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTtgt                                           >  2:228582/1‑151 (MQ=255)                                                                                               gCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTtgtg                                          >  2:662266/1‑151 (MQ=255)                                                                                                  cGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTgcgg                                       >  2:556958/1‑151 (MQ=255)                                                                                                    aTACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTgcggcg                                     <  1:815538/151‑1 (MQ=255)                                                                                                    aTACCGGACTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTGTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTgcggcg                                     >  1:580411/1‑151 (MQ=255)                                                                                                     tACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGgcg                                                                                            >  2:170671/1‑95 (MQ=255)                                                                                                     tACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGgcg                                                                                            <  1:170648/95‑1 (MQ=255)                                                                                                        cGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGTCATCCAGTTGTGCGGCGGTTa                                 <  1:591619/151‑1 (MQ=255)                                                                                                        cGGATTACGCTTCACACGCGATGCGACCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCCGTTGTGCGGCGGtt                                  >  1:764418/1‑150 (MQ=255)                                                                                                             tACGCTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATc                            >  2:446184/1‑151 (MQ=255)                                                                                                                 cTTCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCaaa                         >  2:176096/1‑150 (MQ=255)                                                                                                                   tCACACGCGATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGc                      >  1:86982/1‑151 (MQ=255)                                                                                                                        cgcgATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGt                 <  1:176073/151‑1 (MQ=255)                                                                                                                           gATGCGCCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGc              <  2:563720/151‑1 (MQ=255)                                                                                                                              gcgcCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCg            <  2:834244/150‑1 (MQ=255)                                                                                                                                gcCCTGGCCCGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGAt         >  2:562799/1‑151 (MQ=255)                                                                                                                                       cccGTCATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCCAACGCTCGGTTGCCGGATCGGTACg  >  1:479217/1‑151 (MQ=255)                                                                                                                                            |                                                                                                                                                  AAAGCCGCGCCCAGCGCGGAAACGCCGCACGGGCTGCCCGACAAGCCTGCTCGACCTGAGCGGCATCGGCATCATTGCCTTGCCATAACACCTCGCCCGATACCGGATTACGCTTCACACGCGATGCGCCCTGGCCCGTTATCCAGTCACCGTTAATCCATAAAGTCATGCTGTTTTCTCCTCTGCGCACAGGCGCACCAGACGAACGCGATCCCCGGCGTGGCATTTGAGGGCATCCAGTTGTGCGGCGGTTAAAATCAAACGCTCGGTTGCCGGATCGGTACG  >  NC_000913/1829566‑1829850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑