Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,859,148	A→G	F361S (TTC→TCC)	ydjK ←	putative MFS sugar transporter, membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,859,148	0	A	G	100.0%	227.1 / NA	60	F361S (TTC→TCC)	ydjK	putative MFS sugar transporter, membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/37);  total (23/37)

GGCATAAGGTGCGGCAATACCACTGATTCGCCCTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGAAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGCAATAAAAATACCAAATGGCG  >  NC_000913/1859006‑1859295                                                                                                                                               |                                                                                                                                                    ggCATAAGGTGCGGCAATACCACTGATTCGCCCTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCa                                                                                                                                             <  2:111155/151‑1 (MQ=255)   cATAAGGTGCGGCAATACCACTGATTCGCCCTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAAt                                                                                                                                           >  1:37685/1‑151 (MQ=255)      aaGGTGCGGCAATACCACTGATTCGCCCTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCaa                                                                                                                                        <  1:250062/151‑1 (MQ=255)        ggTGCGGCAATACCACTGATTCGCCCTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACg                                                                                                                                      <  1:235313/151‑1 (MQ=255)            cGGCAATACCACTGATTCGCCCTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTAt                                                                                                                                  <  1:358819/151‑1 (MQ=255)              gCAATACCACTGATTCGCCCTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGa                                                                                                                                <  1:682526/151‑1 (MQ=255)                 aTACCACTGATTCGCCCTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCa                                                                                                                             <  1:401869/151‑1 (MQ=255)                   aCCACTGATTCGCCCTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCaa                                                                                                                            <  1:390204/150‑1 (MQ=255)                     cACTGATTCGCCCTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACAt                                                                                                                         <  2:215528/151‑1 (MQ=255)                      aCTGATTCGCCCTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATa                                                                                                                        <  2:333681/151‑1 (MQ=255)                           ttCGCCCTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGt                                                                                                                   >  1:714478/1‑151 (MQ=255)                           ttCGCCCTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGt                                                                                                                   >  1:636979/1‑151 (MQ=255)                             cGCCCTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGttt                                                                                                                 <  2:609598/151‑1 (MQ=255)                             cGCCCTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGtt                                                                                                                  <  2:586027/150‑1 (MQ=255)                                  tACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGt                                                                                                            >  1:125270/1‑151 (MQ=255)                                   aCCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGt                                                                                                            <  2:766002/150‑1 (MQ=255)                                   aCCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTg                                                                                                           <  2:173527/151‑1 (MQ=255)                                      gCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAAt                                                                                                        <  2:533494/151‑1 (MQ=255)                                        aTTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAg                                                                                                      <  2:770700/151‑1 (MQ=255)                                           cGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGata                                                                                                   <  1:473396/151‑1 (MQ=255)                                             ccAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGatata                                                                                                 <  2:356207/151‑1 (MQ=255)                                             ccAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGatata                                                                                                 <  1:297360/151‑1 (MQ=255)                                               aGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATc                                                                                               <  2:22873/151‑1 (MQ=255)                                                   cGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAg                                                                                           <  1:89884/151‑1 (MQ=255)                                                          aCGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCa                                                                                    <  1:256213/151‑1 (MQ=255)                                                            ggAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAAt                                                                                  >  2:319969/1‑151 (MQ=255)                                                            ggAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAAt                                                                                  <  1:177969/151‑1 (MQ=255)                                                            ggAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAAt                                                                                  <  1:259706/151‑1 (MQ=255)                                                             gAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATc                                                                                 <  2:323998/151‑1 (MQ=255)                                                              aGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATc                                                                                 >  2:740022/1‑150 (MQ=255)                                                                   ggCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGAtt                                                                           >  2:485028/1‑151 (MQ=255)                                                                        ctGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAAt                                                                        <  1:768164/149‑1 (MQ=255)                                                                          gTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAg                                                                      <  2:303188/149‑1 (MQ=255)                                                                          gTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGcc                                                                    >  2:520628/1‑151 (MQ=255)                                                                            cGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATGTCCGAGCACCGCAATCAGGATTAATAGcccc                                                                  >  2:5245/1‑151 (MQ=255)                                                                             ggCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGcccc                                                                  >  2:653296/1‑150 (MQ=255)                                                                                cAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCacac                                                              >  1:80353/1‑151 (MQ=255)                                                                                 aGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACAcc                                                             <  1:426265/151‑1 (MQ=255)                                                                                          ggACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGtttt                                                    <  2:644372/151‑1 (MQ=255)                                                                                          ggACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGtttt                                                    <  1:349530/151‑1 (MQ=255)                                                                                                      gcGAGGCATAGCCGACGTACATATAGACGTAAGTAATCAGGGAGAAACCAATCACCGTTATGAGCAACATACTGGTTTGCAGTGAATAGATTTATCCGACCACCGCAATCAGGAGTAATATCCCCAAACCCATTGTTTTACGCGGAAtttt                                        >  1:778891/2‑151 (MQ=255)                                                                                                        gAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTAt                                      >  2:529532/1‑151 (MQ=255)                                                                                                          ggCATAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATcc                                    <  1:401559/151‑1 (MQ=255)                                                                                                             aTAGCAAACGTACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATc                                 >  2:123565/1‑151 (MQ=255)                                                                                                                       tACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATgg                       >  2:692897/1‑151 (MQ=255)                                                                                                                       tACATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATgg                       >  2:852006/1‑151 (MQ=255)                                                                                                                         cATATAGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGc                      >  1:414214/1‑150 (MQ=255)                                                                                                                            ataGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGCAAt                   >  2:205350/1‑150 (MQ=255)                                                                                                                              aGACGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGCAATaa                 >  2:319492/1‑150 (MQ=255)                                                                                                                                 cGAAAGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGCAATAAAAAt             <  1:274553/151‑1 (MQ=255)                                                                                                                                   aaaGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGCAATAAAAATAc           <  2:116855/151‑1 (MQ=255)                                                                                                                                   aaaGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGCAATAAAAATAc           >  1:617597/1‑151 (MQ=255)                                                                                                                                    aaGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGCAATAAAAATAcc          <  2:251006/151‑1 (MQ=255)                                                                                                                                     aGTAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGCAATAAAAATACCa         <  1:226140/151‑1 (MQ=255)                                                                                                                                       tAATCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGCAATAAAAATACCaa        >  1:260425/1‑150 (MQ=255)                                                                                                                                         aTCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGCAATAAAAATACCAAGTg     <  2:464866/151‑1 (MQ=255)                                                                                                                                          tCAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATGAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGCAATAAAAATACCAAATg     <  1:845283/150‑1 (MQ=255)                                                                                                                                           cAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGCAATAAAAATACCAAATGgc   >  2:350176/1‑151 (MQ=255)                                                                                                                                           cAGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGCAATAAAAATACCAAATGgc   >  1:523802/1‑151 (MQ=255)                                                                                                                                            aGGGAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGCAATAAAAATACCAAATGgcg  <  1:131273/151‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                    GGCATAAGGTGCGGCAATACCACTGATTCGCCCTACCGCATTCGCCAGACCGGAGCCACGGAGTTTGGCCTCTGTCGGCCAGATTTCAGGGACATACACTGCCGAGGCATAGCAAACGTACATATAGACGAAAGTAATCAGGAAGAAACCAATCAACGTTATGAGCAACATACTGGTTTGCAGTGAATAGATATATCCGAGCACCGCAATCAGGATTAATAGCCCCACACCCATTGTTTTACGCGGAATTTTATCCATCACCAGCATGGCAATAAAAATACCAAATGGCG  >  NC_000913/1859006‑1859295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑