Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	300,337	C→T	D200N (GAT→AAT)	paoC ←	PaoABC aldehyde oxidoreductase, Moco‑containing subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	300,337	0	C	T	100.0%	175.1 / NA	47	D200N (GAT→AAT)	paoC	PaoABC aldehyde oxidoreductase, Moco‑containing subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/15);  total (32/15)

ACAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATT  >  NC_000913/300196‑300484                                                                                                                                              |                                                                                                                                                    aCAGCTTGCCGCCAACCCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGc                                                                                                                                            <  1:856329/151‑1 (MQ=255)  cAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGcc                                                                                                                                           >  1:749097/1‑151 (MQ=255)     cTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGAACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATc                                                                                                                                        >  1:130296/1‑151 (MQ=255)       tGCCGCCAAACCCTCAGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCAATCg                                                                                                                                       >  1:678480/1‑150 (MQ=255)           gccAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGc                                                                                                                                   >  1:356876/1‑150 (MQ=255)             cAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATg                                                                                                                                >  1:164569/1‑151 (MQ=255)              aaaCCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGc                                                                                                                               >  1:684742/1‑151 (MQ=255)               aaCCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCg                                                                                                                              >  2:86500/1‑151 (MQ=255)                   cTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTc                                                                                                                          >  1:550656/1‑151 (MQ=255)                     ccgccgATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTcc                                                                                                                         >  1:840524/1‑150 (MQ=255)                     ccgccgATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTc                                                                                                                          <  2:682432/149‑1 (MQ=255)                                cGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATgg                                                                                                             >  2:285322/1‑151 (MQ=255)                                   ggAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGctc                                                                                                          >  1:319524/1‑151 (MQ=255)                                   ggAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGctc                                                                                                          <  1:570373/151‑1 (MQ=255)                                         aataCGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTcc                                                                                                    >  2:97921/1‑151 (MQ=255)                                          ataCGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCg                                                                                                   >  2:144683/1‑151 (MQ=255)                                               cacaTTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTc                                                                                              <  2:691776/151‑1 (MQ=255)                                               cacaTTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTc                                                                                              >  1:848008/1‑151 (MQ=255)                                                acaTTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCg                                                                                             <  1:97898/151‑1 (MQ=255)                                                 caTTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCgt                                                                                            <  2:312974/151‑1 (MQ=255)                                                 caTTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCgt                                                                                            >  1:107971/1‑151 (MQ=255)                                                  aTTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCgtg                                                                                           <  1:692183/151‑1 (MQ=255)                                                    tctcCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCgtgt                                                                                          <  1:22075/150‑1 (MQ=255)                                                     ccccACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATGATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCCCCATATGGCTCTGGTCCGGGGTCgcgt                                                                                          >  1:65112/3‑149 (MQ=255)                                                         aCGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTgtagg                                                                                       >  1:677938/1‑148 (MQ=255)                                                           gggAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGc                                                                                   >  1:819530/1‑150 (MQ=255)                                                               aCTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGAGGTCGTGTAGTTAGc                                                                                   <  1:476420/146‑1 (MQ=255)                                                                 tttCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATc                                                                            >  1:27056/1‑151 (MQ=255)                                                                     aGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGGCGTGTAGGTAGCATCAATCTTc                                                                         >  2:646724/1‑150 (MQ=255)                                                                                 aGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAgg                                                            >  1:92673/1‑151 (MQ=255)                                                                                     cGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGa                                                         >  2:667004/1‑150 (MQ=255)                                                                                                   tCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGt                                          >  2:629089/1‑151 (MQ=255)                                                                                                    cAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTc                                         >  2:101688/1‑151 (MQ=255)                                                                                                        catcTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCAcc                                      <  1:218263/150‑1 (MQ=255)                                                                                                        catcTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCACCg                                     <  1:262342/151‑1 (MQ=255)                                                                                                        catcTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCACCg                                     >  2:509734/1‑151 (MQ=255)                                                                                                        catcAGATTTGAGGTCAAGAGGGTAAGCGTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCTCCATATTGCTCTGGACCGGGGTCGTGAAGGACGCATACATCTTCATCCCAGCGGAGGTGAAAGCCTCGTCAAAGTCACCg                                     >  1:545413/1‑151 (MQ=255)                                                                                                         atcTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCACCg                                     >  2:668642/1‑150 (MQ=255)                                                                                                              aTTTGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCACCGACGttt                               >  2:183170/1‑151 (MQ=255)                                                                                                               tttGAGGTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCACCGACGtttt                              >  1:618996/1‑151 (MQ=255)                                                                                                                     gTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCACCGACGTTTTTGTCgg                        <  2:550693/151‑1 (MQ=255)                                                                                                                     gTCCAGAGGGTAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCACCGACGTTTTTGTCgg                        <  2:654961/151‑1 (MQ=255)                                                                                                                               tAAGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCACCGACGTTTTTGTCGGGCGTGTCtt               <  2:492413/150‑1 (MQ=255)                                                                                                                                aaGCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCACCGACGTTTTTGTCGGGCGTGTCTTcc             >  2:177571/1‑151 (MQ=255)                                                                                                                                  gCTTATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCACCGACGTTTTTGTCGGGCGTGTCTTCcgg           <  2:857160/151‑1 (MQ=255)                                                                                                                                     tATTTCCATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCACCGACGTTTTTGTCGGGCGTGTCTTCcggcgg        >  1:427026/1‑151 (MQ=255)                                                                                                                                        ttccattccaGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCt      <  1:740329/150‑1 (MQ=255)                                                                                                                                           cATTCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGAtt  >  1:434771/1‑151 (MQ=255)                                                                                                                                              |                                                                                                                                                    ACAGCTTGCCGCCAAACCCTCCGCCGATATACGGGGAGATAATACGCACATTCTCCACGGGAACTTTCAGCGTTTTTGCCAGATCGGTGCGGCACCAGTCAATCATCTGATTTGAGGTCCAGAGGGTAAGCTTATTTCCATCCCAGACGGCCATCGAGGCATGCGGCTCCATCGCCATATGGCTCTGGTCCGGGGTCGTGTAGGTAGCATCAATCTTCACCGCAGCGGAGGTGAAAGCCCCGTCAAAGTCACCGACGTTTTTGTCGGGCGTGTCTTCCGGCGGCTGATT  >  NC_000913/300196‑300484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑