Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,941,228	A→G	G136G (GGT→GGC)	mepM ←	murein DD‑endopeptidase, space‑maker hydrolase, septation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,941,228	0	A	G	97.7%	141.3 / NA	43	G136G (GGT→GGC)	mepM	murein DD‑endopeptidase, space‑maker hydrolase, septation protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (21/21);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

GTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCG  >  NC_000913/1941081‑1941369                                                                                                                                                    |                                                                                                                                              gTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGcc                                                                                                                                             <  1:404244/150‑1 (MQ=255)                 tgctgcATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACg                                                                                                                              <  1:347520/149‑1 (MQ=255)                     gcATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTc                                                                                                                        >  2:669369/1‑151 (MQ=255)                         ttCTCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATGGGTTCGGGTTTCACGGCGATACACTTCCCAGGTCACGCGCTGCCGCGCCCCGACCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTCCGCAATTCtt                                                                                                                      <  1:71750/149‑1 (MQ=255)                          tCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTg                                                                                                                    <  2:432801/150‑1 (MQ=255)                           cGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTc                                                                                                                  <  1:323737/151‑1 (MQ=255)                            gCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCg                                                                                                                 >  2:771033/1‑151 (MQ=255)                                    ttttAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCa                                                                                                           <  2:750389/149‑1 (MQ=255)                                     tttAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGcc                                                                                                        >  2:40079/1‑151 (MQ=255)                                     tttAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGc                                                                                                         <  1:153154/150‑1 (MQ=255)                                      ttAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGcc                                                                                                        >  2:263551/1‑150 (MQ=255)                                         aaaCCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGtt                                                                                                    >  1:603219/1‑151 (MQ=255)                                           aCCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTgg                                                                                                  >  1:661131/1‑151 (MQ=255)                                             cGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCGGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGt                                                                                                <  2:782368/151‑1 (MQ=255)                                                 aGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGat                                                                                             <  2:637578/150‑1 (MQ=255)                                                             gTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCa                                                                                <  1:458903/151‑1 (MQ=255)                                                               cATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAAt                                                                              <  2:89622/151‑1 (MQ=255)                                                                      tCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATAACCCATATCAATACCATAc                                                                       >  1:155358/1‑151 (MQ=255)                                                                        gggTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTg                                                                     >  1:392271/1‑151 (MQ=255)                                                                         gggttCACGAAGAGACACTTCCCAGGTGAGGCGATGCAGTTCGCCGTCCGCGGGTAATGTCCAGGAGAGTGGTTGGCCGATTTTCAGGGTACGCAATTCTTTGTCGGCAGCAGCAAGTTGGGTGATATCACCCATATCAATACCATACTg                                                                     >  2:545555/4‑150 (MQ=255)                                                                         ggTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCAGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTg                                                                     >  2:545554/1‑150 (MQ=255)                                                                          gTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGAt                                                                   <  2:668846/151‑1 (MQ=255)                                                                                  acgaGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAAtg                                                           <  2:62179/151‑1 (MQ=255)                                                                                     agaCACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAAtgctg                                                        >  1:13138/1‑151 (MQ=255)                                                                                        cacTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCtt                                                     <  1:165387/151‑1 (MQ=255)                                                                                                 ggTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCg                                            >  2:733611/1‑151 (MQ=255)                                                                                                    gAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCa                                         <  1:283629/151‑1 (MQ=255)                                                                                                       gcgcTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTg                                      <  1:694176/151‑1 (MQ=255)                                                                                                            gCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAaac                                 >  1:451505/1‑151 (MQ=255)                                                                                                                        ccGCGGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATg                     <  1:367135/151‑1 (MQ=255)                                                                                                                           cgGTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCAc                  >  2:107322/1‑151 (MQ=255)                                                                                                                            ggTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACg                 >  2:253045/1‑151 (MQ=255)                                                                                                                             gTTAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATc                                             <  1:578758/122‑1 (MQ=255)                                                                                                                              ttAATGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGcc               >  2:630881/1‑151 (MQ=255)                                                                                                                                aaTGTCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGccc              <  2:706578/150‑1 (MQ=255)                                                                                                                                aaTGACCACGAGAGTGCTTGGCAGATTTTCAGGTTACGCAATTCTATGTCGACCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTCAGAATGCTACTTAGCATGTCGCCAGTCGAAACAACATATTCAAGCActctcg             <  1:251137/151‑6 (MQ=255)                                                                                                                                    tCCAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTc         >  2:778128/1‑151 (MQ=255)                                                                                                                                     ccAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCa        <  1:629625/151‑1 (MQ=255)                                                                                                                                      cAGGAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCAc       >  1:775987/1‑151 (MQ=255)                                                                                                                                        ggAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCg     >  2:651944/1‑151 (MQ=255)                                                                                                                                        ggAGAGTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCg     <  2:392303/151‑1 (MQ=255)                                                                                                                                          agagTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGc   >  1:215384/1‑151 (MQ=255)                                                                                                                                            agTTGTTGGCCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCg  >  2:361312/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                              GTTAACCCACTCTCCTTGCTGCATTTCGCTGGTCATTTTAAAACCGTTAGCGGCAGTACGGTCATAGGTTCGGGTTTCACGACGAGACACTTCCCAGGTGAGGCGCTGCAGTTCGCCGTCCGCGGTTAATGTCCAGGAGAGTTGTTGACCGATTTTCAGGTTACGCAATTCTTTGTCGGCCGCAGCCAGTTGGGTGATATCACCCATATCAATACCATACTGATTGAGAATGCTGCTTAGCGTATCGCCAGTGGAAACAACATATTCATGCACGCCCGCTTCACCGGCG  >  NC_000913/1941081‑1941369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑