Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,059,573	G→A	R40C (CGC→TGC)	yeeO ←	putative multdrug exporter, MATE family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,059,573	0	G	A	100.0%	204.6 / NA	55	R40C (CGC→TGC)	yeeO	putative multdrug exporter, MATE family
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/28);  total (27/28)

ATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCGCCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGACCTTAATTGCAGAAAGTGAAACCACATTTCAAATAATGAGGGAGAATCAGCAAAGCT  >  NC_000913/2059425‑2059720                                                                                                                                                     |                                                                                                                                                    aTAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCAcc                                                                                                                                                   >  1:163999/1‑151 (MQ=255)    aTGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCAcc                                                                                                                                                   >  2:349774/1‑148 (MQ=255)      gACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGaac                                                                                                                                              <  1:685281/151‑1 (MQ=255)      gACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGaa                                                                                                                                               <  1:481691/150‑1 (MQ=255)       aCCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGaaca                                                                                                                                             <  2:805218/151‑1 (MQ=255)        ccATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGaacaa                                                                                                                                            <  2:363957/151‑1 (MQ=255)            atTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACt                                                                                                                                        >  1:789004/1‑151 (MQ=255)                 aGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGt                                                                                                                                    >  2:359648/1‑150 (MQ=255)                 aGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGt                                                                                                                                    >  1:338817/1‑150 (MQ=255)                       ccGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCtt                                                                                                                             >  2:809627/1‑151 (MQ=255)                         gCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGc                                                                                                                           <  1:782388/151‑1 (MQ=255)                               cccACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGTACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTa                                                                                                                      <  1:566965/150‑1 (MQ=255)                                  aCGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGGGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATATAGCAACAGAACAACACTTTGTAGCTCTTGCGTTTAGCGt                                                                                                                  >  1:367153/1‑151 (MQ=255)                                    gCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGc                                                                                                                <  1:101481/151‑1 (MQ=255)                                     ccGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGcc                                                                                                               >  1:303403/1‑151 (MQ=255)                                         ccATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCg                                                                                                           <  1:215809/151‑1 (MQ=255)                                             cGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTg                                                                                                       <  2:342101/151‑1 (MQ=255)                                                     ttCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGCCACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGTTTATCTCGCACCAGAACAACTCTTTGGAGCTCTTGCGTTTAGCGTGCCAACGAGTGCCGTg                                                                                                  <  1:181937/148‑1 (MQ=255)                                                      tCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGaac                                                                                               <  1:57837/150‑1 (MQ=255)                                                        ccagccagCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGaacaac                                                                                            >  2:562982/1‑151 (MQ=255)                                                         cagccagcTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAAcc                                                                                           >  1:46227/1‑151 (MQ=255)                                                             cagcTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGc                                                                                       <  2:416419/151‑1 (MQ=255)                                                             cagcTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGc                                                                                       >  2:457081/1‑151 (MQ=255)                                                                  gACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTaa                                                                                   >  2:282520/1‑150 (MQ=255)                                                                  gACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTa                                                                                    <  2:639064/149‑1 (MQ=255)                                                                       gAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGc                                                                                >  2:561017/1‑148 (MQ=255)                                                                            gTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAgagg                                                                           >  2:54525/1‑148 (MQ=255)                                                                                    aaCCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCaa                                                                 >  2:405426/1‑150 (MQ=255)                                                                                       ccccATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGcc                                                             >  1:46010/1‑151 (MQ=255)                                                                                        cccATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCg                                                            >  2:799741/1‑151 (MQ=255)                                                                                        cccATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCg                                                            >  1:56708/1‑151 (MQ=255)                                                                                             cAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGACCtt                                                       <  1:223945/151‑1 (MQ=255)                                                                                              aaCAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGACCtt                                                       >  1:616947/1‑150 (MQ=255)                                                                                              aaCAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGACCtt                                                       >  1:572024/1‑150 (MQ=255)                                                                                               aCAGGACACAGGCATTCTCCATGAAGATAGCAACAGCAAGCGGGTTTATCCCGCACCAGAACAACACTTTGTAGCTCTAGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGACCTTaa                                                     <  1:11584/151‑1 (MQ=255)                                                                                                  ggACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGACCTTAATTg                                                  >  2:443777/1‑151 (MQ=255)                                                                                                    acacAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGACCTTAATTGCa                                                <  1:734316/151‑1 (MQ=255)                                                                                                      acaGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGACCTTAATTGCAGa                                              <  2:419001/151‑1 (MQ=255)                                                                                                       caGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGACCTTAATTGCAGa                                              >  2:696152/1‑150 (MQ=255)                                                                                                       caGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGACCTTAATTGCAGa                                              >  2:85199/1‑150 (MQ=255)                                                                                                       caGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGACCTTAATTGCAGa                                              >  1:700494/1‑150 (MQ=255)                                                                                                            aTTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGACCTTAATTGCAGAAAGTGa                                        <  1:262336/151‑1 (MQ=255)                                                                                                                 ccATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGACCTTAATTGCAGAAAGTGaaa                                      <  2:210060/148‑1 (MQ=255)                                                                                                                 ccATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTACGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGACCTTAATTGCAGAAAGTGaaa                                      <  1:603258/148‑1 (MQ=255)                                                                                                                         aTAGGAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGACCTTAATTGCAGAAAGTGAAACCACATTTCaa                           <  1:762214/151‑1 (MQ=255)                                                                                                                            ggAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGACCTTAATTGCAGAAAGTGAAACCACATTTCAaata                        <  1:579136/151‑1 (MQ=255)                                                                                                                            ggAACAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGACCTTAATTGCAGAAAGTGAAACCACATTTCAaata                        <  1:422092/151‑1 (MQ=255)                                                                                                                              aaCAGCAAGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGACCTTAATTGCAGAAAGTGAAACCACATTTCAaataat                      >  2:635431/1‑151 (MQ=255)                                                                                                                                     aGCGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGACCTTAATTGCAGAAAGTGAAACCACATTTCAAATAATGAGGgag               <  2:287053/151‑1 (MQ=255)                                                                                                                                       cGGGGTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGACCTTAATTGCAGAAAGTGAAACCACATTTCAAATAATGAGGGAGaa             <  2:295401/151‑1 (MQ=255)                                                                                                                                         gggTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGACCTTAATTGCAGAAAGTGAAACCACATTTCAAATAATGAGGGAGAATc           <  2:636845/151‑1 (MQ=255)                                                                                                                                           gTTATCTCGCACCAGAACAACTCTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGTCCTTAATTGCAGAAAGTGAAACCACATTTCAAATAATGAGGGAGATTCAg         <  1:736062/151‑1 (MQ=255)                                                                                                                                           gTTATCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGACCTTAATTGCAGAAAGTGAAACCACATTTCAAATAATGAGGGAGAATCAg         <  1:85176/151‑1 (MQ=255)                                                                                                                                              aTCTCGCACCAGAACAACACTTTGTAGCTCTTGCGTTTGGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGACCTTAATTGCAGAAAGTGAAACCACATTTCAAATAATGAGGGAGAATCAGCa       >  2:760735/1‑150 (MQ=255)                                                                                                                                               tctcGCACCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGACCTTAATTGCAGAAAGTGAAACCACATTTCAAATAATGAGGGAGAATCAGCaa      >  1:10261/1‑150 (MQ=255)                                                                                                                                                  cgcaCCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGACCTTAATTGCAGAAAGTGAAACCACATTTCAAATAATGAGGGAGAATCAGCAAAGCt  >  2:313648/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    ATAATGACCATATTGAAGCTGTCCGCCAATCCCACGCCGGCCATCGCATCTTTTCCCAGCCAGCTGACCAGAAAAGTGCTCAGAACCCCCATCAACAGGACACAGGCATTCTCCATGAAGATAGGAACAGCAAGCGGGGTTATCTCGCGCCAGAACAACACTTTGTAGCTCTTGCGTTTAGCGTGCCAGCGAGTGCCGTGAACAACCTGGCGTAAAGCAGAGGAGATATTCAAAGCCGACCTTAATTGCAGAAAGTGAAACCACATTTCAAATAATGAGGGAGAATCAGCAAAGCT  >  NC_000913/2059425‑2059720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑