Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,082,465 G→A S28L (TCA→TTA)  dacD ← D‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6b

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,082,4650GA100.0% 174.0 / NA 47S28L (TCA→TTA) dacDD‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6b
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/31);  total (16/31)

TAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTGAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTG  >  NC_000913/2082319‑2082609
                                                                                                                                                  |                                                                                                                                                
tAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGGGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTaaaa                                                                                                                                               >  1:540234/1‑150 (MQ=255)
 aGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAaaaa                                                                                                                                              >  2:845189/1‑150 (MQ=255)
 aGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAaaaa                                                                                                                                              <  1:537605/150‑1 (MQ=255)
  gcgcGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAg                                                                                                                                            <  1:682650/151‑1 (MQ=255)
      gATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAAt                                                                                                                                        >  2:726635/1‑151 (MQ=255)
       aTCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGAACCGGCATGAATATCTGGTGGCTGAGGTAAAAAAGGaa                                                                                                                                         >  1:531606/1‑149 (MQ=255)
       aTCCACGACATAACCCGTCATCAGATTTGTCAGGCTGGCGGGATTGTGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTATAATCCATAAATGCCCAGAACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATg                                                                                                                                       <  1:736020/151‑1 (MQ=255)
         ccACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGtt                                                                                                                                     >  2:797013/1‑151 (MQ=255)
              aCATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTccg                                                                                                                                <  1:788298/151‑1 (MQ=255)
                            caGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGa                                                                                                                   <  1:112112/150‑1 (MQ=255)
                               cTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGGAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACg                                                                                                                 >  2:338161/1‑149 (MQ=255)
                                  tGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCGGCGGTGAGGATCTGACCGGTGGTGTAATCCATAAATACCCAGGACCCGGCATGAATCTCAGGAGGCTGAGGTAAAAAAGGAATTTTTACCGCCGCAAAACCAGACGATaa                                                                                                             <  1:758938/150‑1 (MQ=255)
                                  tGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATaa                                                                                                             <  1:324278/150‑1 (MQ=255)
                                             cGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGaa                                                                                                 <  1:140474/151‑1 (MQ=255)
                                              gggATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGAGTAATCCATCAATACCCAGGACCCGGCAAGAGTCTCTGGAGGCTGATGGAAAAAAGGACAGTTTTCCGCCGCAATACCAGACGATAAGTTAAAAACGa                                                                                                  >  1:387813/1‑149 (MQ=255)
                                               ggATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAAc                                                                                                <  2:332427/150‑1 (MQ=255)
                                                 aTTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACaa                                                                                              >  2:745564/1‑150 (MQ=255)
                                                     cgcTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAaagaag                                                                                         <  2:303530/151‑1 (MQ=255)
                                                     cgcTGTTGATGCCCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCAGGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGTTAAGTTAAAAACGAACaaa                                                                                             <  2:335221/147‑1 (MQ=255)
                                                         gTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAagca                                                                                       <  1:85766/149‑1 (MQ=255)
                                                            gATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCaa                                                                                   <  2:575354/150‑1 (MQ=255)
                                                             aTGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCaat                                                                                  >  1:727613/1‑150 (MQ=255)
                                                               gCTCATTGCCCGCGGTGAGGATCTGACCGGTGGTGTAAACCATCAATACCCAGGACCCAGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTACCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCaataa                                                                                <  1:845212/150‑1 (MQ=255)
                                                                cTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCaataat                                                                               <  2:727656/150‑1 (MQ=255)
                                                                  cATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAataata                                                                              >  1:571643/1‑149 (MQ=255)
                                                                   aTTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAataata                                                                              >  1:317988/1‑148 (MQ=255)
                                                                        ccGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCg                                                                      <  2:703566/151‑1 (MQ=255)
                                                                         cgcgGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGt                                                                     <  1:339885/151‑1 (MQ=255)
                                                                                     cTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCaa                                                          >  1:584262/1‑150 (MQ=255)
                                                                                       gACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCaa                                                          <  1:536502/148‑1 (MQ=255)
                                                                                         ccGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAAccc                                                      <  1:741067/150‑1 (MQ=255)
                                                                                          cGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTc                                                    <  2:574621/151‑1 (MQ=255)
                                                                                           ggtggtGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCa                                                   <  2:490797/151‑1 (MQ=255)
                                                                                                          cAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGc                                    <  1:242626/151‑1 (MQ=255)
                                                                                                                  aGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCttt                            >  1:79464/1‑151 (MQ=255)
                                                                                                                  aGGACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCttt                            >  2:63865/1‑151 (MQ=255)
                                                                                                                    gACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCttttt                          <  2:5532/151‑1 (MQ=255)
                                                                                                                    gACCCGGCATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCttttt                          <  1:614996/151‑1 (MQ=255)
                                                                                                                           cATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGaaa                   <  1:81671/151‑1 (MQ=255)
                                                                                                                           cATGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGaa                    >  1:119275/1‑150 (MQ=255)
                                                                                                                            aTGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAAt                  <  2:241859/151‑1 (MQ=255)
                                                                                                                            aTGAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAAt                  <  1:257024/151‑1 (MQ=255)
                                                                                                                              gAATCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATAc                >  2:555415/1‑151 (MQ=255)
                                                                                                                               aaTCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACt               <  1:155162/151‑1 (MQ=255)
                                                                                                                                aTCTCTGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACtt              >  1:835957/1‑151 (MQ=255)
                                                                                                                                    ctGGAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTAt            <  1:785606/149‑1 (MQ=255)
                                                                                                                                      ggAGGCTGAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGa          <  1:471840/149‑1 (MQ=255)
                                                                                                                                             gAGGTAAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAActggctg  <  2:4410/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGCGGTGAGGATCTGACCGGTGGTGTAATCCATCAATACCCAGGACCCGGCATGAATCTCTGGAGGCTGAGGTGAAAAAGGAATGTTTTCCGCCGCAAAACCAGACGATAAGTTAAAAACGAACAAAGAAGCAGCAATAATAAGACGGCGTTTCAACAGCAAACCCTCAGGAGTTTCAAATAGCTGTTCTTTTTACGGAAATACTTATGAACTGGCTG  >  NC_000913/2082319‑2082609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑