Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,104,750	T→C	H112R (CAC→CGC)	wbbJ ←	putative lipopolysaccharide biosynthesis O‑acetyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,104,750	0	T	C	97.8%	150.0 / ‑4.1	45	H112R (CAC→CGC)	wbbJ	putative lipopolysaccharide biosynthesis O‑acetyl transferase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (16/28);  total (16/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.55e-01

GCGCCGACTACGACTCCATTACCAATAATTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGTGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAACTTGCACATTATCGGAAAAAAAAATCACGCCACGTCC  >  NC_000913/2104603‑2104895                                                                                                                                                    |                                                                                                                                                  gcgcCGACTACGACTCCATTACCAATAATTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCt                                                                                                                                                <  1:516773/151‑1 (MQ=255)  cgcCGACTACGACTCCATTACCAATAATTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCtt                                                                                                                                               >  1:459808/1‑151 (MQ=255)     cGACTACGACTCCATTACCAATAATTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTaaa                                                                                                                                            <  1:143263/151‑1 (MQ=255)       aCTACGACTCCATTACCAATAATTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAg                                                                                                                                           <  1:675214/150‑1 (MQ=255)            gACTCCATTACCAATAATTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCg                                                                                                                                     >  2:795537/1‑151 (MQ=255)               tCCATTACCAATAATTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTg                                                                                                                                   >  2:118542/1‑150 (MQ=255)                     aCCAATAATTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCGACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGa                                                                                                                            <  2:539362/151‑1 (MQ=255)                       cAATAATTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATc                                                                                                                          <  2:758494/151‑1 (MQ=255)                       cAATAATTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATc                                                                                                                          <  2:737442/151‑1 (MQ=255)                         ataatTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCgg                                                                                                                        <  2:132964/151‑1 (MQ=255)                                     ggCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGTGCTTAAAGGAACCGTGATTATGATCGGTAATAAAtactt                                                                                                            <  2:625585/151‑1 (MQ=255)                                        aaaaCCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAAtactttac                                                                                                         >  2:258671/1‑151 (MQ=255)                                           aCCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTg                                                                                                      <  1:272102/151‑1 (MQ=255)                                             cGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCa                                                                                                    <  1:858675/151‑1 (MQ=255)                                              gTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCa                                                                                                    >  1:263955/1‑150 (MQ=255)                                                  caTTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATa                                                                                                 >  1:571725/1‑149 (MQ=255)                                                        cACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTa                                                                                           <  1:221983/149‑1 (MQ=255)                                                         aCCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATcc                                                                                        <  2:219309/151‑1 (MQ=255)                                                            caaccaaACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGa                                                                                     <  1:566300/151‑1 (MQ=255)                                                               ccaaACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCt                                                                                  <  2:631062/151‑1 (MQ=255)                                                                caaACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTa                                                                                 <  1:170959/151‑1 (MQ=255)                                                                         tGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGc                                                                        >  1:317204/1‑151 (MQ=255)                                                                          ggCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAAc                                                                          <  2:332424/148‑1 (MQ=255)                                                                          ggCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGct                                                                       <  1:358542/151‑1 (MQ=255)                                                                                 tACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAAtt                                                                >  2:518535/1‑151 (MQ=255)                                                                                   caacaGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTg                                                               >  1:139951/1‑150 (MQ=255)                                                                                    aacaGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAg                                                             <  1:197504/151‑1 (MQ=255)                                                                                     acaGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAgg                                                            <  2:235064/151‑1 (MQ=255)                                                                                      caGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGc                                                           >  2:58249/1‑151 (MQ=255)                                                                                           gAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGatat                                                      <  1:589578/151‑1 (MQ=255)                                                                                            aaGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATg                                                     >  2:324460/1‑151 (MQ=255)                                                                                            aaGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATg                                                     >  2:456309/1‑151 (MQ=255)                                                                                                   cAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAg                                              <  2:208436/151‑1 (MQ=255)                                                                                                     aCGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTc                                            >  2:739500/1‑151 (MQ=255)                                                                                                         gcgcATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGtt                                         <  1:37405/150‑1 (MQ=255)                                                                                                          cgcATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTaa                                       <  1:789415/151‑1 (MQ=255)                                                                                                             aTGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAACtt                                    <  2:572472/151‑1 (MQ=255)                                                                                                                  tGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAACTTGcac                                >  2:640847/1‑150 (MQ=255)                                                                                                                  tGGAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAACTTGcac                                >  2:713037/1‑150 (MQ=255)                                                                                                                    gAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAACTTGCACAtt                             >  2:772687/1‑151 (MQ=255)                                                                                                                    gAGGTATATTTGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAACTTGCACAtt                             <  2:94153/151‑1 (MQ=255)                                                                                                                           atTTGGCGAACTCATTGGATCAGAGCGCGTAAAGGACCCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGTCCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGGCGCTAACTTGCACATTATCGGaa                      <  1:529635/151‑1 (MQ=255)                                                                                                                              tGGCGAACTCATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAACTTGCACATTATCGGaaaa                    <  1:235816/150‑1 (MQ=255)                                                                                                                                       cATTGGATCAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAACTTGCACATTATCGGAAAAAAAAATCAc           <  2:116987/150‑1 (MQ=255)                                                                                                                                               cAGAGCGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAACTTGCACATTATCGGAAAAAAAAATCACGCCACGTcc  <  1:541311/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  GCGCCGACTACGACTCCATTACCAATAATTGTTCCAGGCAAAACCGTCACATTCTCACCCAACCAAACCCTCTGGCCAATTACAACAGCTGAAGATTCCAACGTGCGCATGTCTGGAGGTATATTTGGCGAACTCATTGGATCAGAGTGCTTAAAGGAACCGTGATTATGATCGGTAATAAATACTTTACTTGCAATAAGCGTATCCCGACCTATCGTAACGCTCTCAATTGAGGCGATATGAACATAGTCGTTAACTTGCACATTATCGGAAAAAAAAATCACGCCACGTCC  >  NC_000913/2104603‑2104895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑