Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,152,439	C→T	G12R (GGA→AGA)	yegK ←	ser/thr phosphatase‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,152,439	0	C	T	100.0%	170.2 / NA	44	G12R (GGA→AGA)	yegK	ser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/22);  total (22/22)

GCGAGCATCGCGCCTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTG  >  NC_000913/2152291‑2152579                                                                                                                                                     |                                                                                                                                             gCGAGCATCGCGCCTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTaa                                                                                                                                            <  2:88393/151‑1 (MQ=255)         cgcgCCTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGa                                                                                                                                     <  1:158689/150‑1 (MQ=255)         cgcgCCTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGAt                                                                                                                                    >  2:682141/1‑151 (MQ=255)               ttCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCAt                                                                                                                                >  1:292716/1‑149 (MQ=255)                tCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAg                                                                                                                              <  2:290138/150‑1 (MQ=255)                   ccACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTTGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCa                                                                                                                          <  1:545444/151‑1 (MQ=255)                    cACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAg                                                                                                                         <  2:48225/151‑1 (MQ=255)                              gaCACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTc                                                                                                               >  1:391554/1‑151 (MQ=255)                                    gcctgcGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTg                                                                                                         <  1:659713/151‑1 (MQ=255)                                     cctgcGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGa                                                                                                        <  1:672218/151‑1 (MQ=255)                                      ctgcGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGAc                                                                                                       >  1:629025/1‑151 (MQ=255)                                       tgcGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACg                                                                                                      >  1:161258/1‑151 (MQ=255)                                        gcgcCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGt                                                                                                     >  1:647349/1‑151 (MQ=255)                                             aTCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGc                                                                                                >  1:349924/1‑151 (MQ=255)                                                aGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCtt                                                                                             <  1:442287/151‑1 (MQ=255)                                                     cAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGgc                                                                                        >  1:59128/1‑151 (MQ=255)                                                        aTACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATCGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCt                                                                                     <  2:483104/151‑1 (MQ=255)                                                          aCCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTc                                                                                   <  1:203560/151‑1 (MQ=255)                                                           ccGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTcc                                                                                  >  1:72619/1‑151 (MQ=255)                                                            cGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTcc                                                                                  >  1:788507/1‑150 (MQ=255)                                                                    atGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACt                                                                         <  1:42823/151‑1 (MQ=255)                                                                      ggCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTc                                                                       >  2:489661/1‑151 (MQ=255)                                                                       gctgctGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTcc                                                                      >  1:603445/1‑151 (MQ=255)                                                                              gTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCg                                                               <  2:773395/151‑1 (MQ=255)                                                                                cATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCgg                                                              <  2:364350/150‑1 (MQ=255)                                                                                          ggCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGTGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCggg                                                   <  1:64751/151‑1 (MQ=255)                                                                                          ggCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCggg                                                   <  1:64728/151‑1 (MQ=255)                                                                                           gCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCggg                                                   >  1:306463/1‑150 (MQ=255)                                                                                                cTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCg                                             >  2:663851/1‑151 (MQ=255)                                                                                                 tGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCg                                             >  2:426989/1‑150 (MQ=255)                                                                                                      cTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGc                                        >  2:858565/1‑150 (MQ=255)                                                                                                        ggcaggcaTCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACg                                         <  2:391586/147‑1 (MQ=255)                                                                                                        ggcaggcaTCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCa                                       <  2:161281/149‑1 (MQ=255)                                                                                                              caTCCTGACAAGGACAGTCCGAGCTGATGTGCGACGCTCTAACGGTTGATGCATAGACCAGACCCCAGCTCACACTGACATCCAGCCTTTTGGCGCTTCCCGCACAACTTCCGTTCTCTGCGTGGCGCGGGCAATCGAACg                                         >  1:344437/1‑141 (MQ=255)                                                                                                                    gACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATaa                          >  2:418329/1‑150 (MQ=255)                                                                                                                    gACAAGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAAc                         >  2:545826/1‑151 (MQ=255)                                                                                                                       aaGGCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAg                      <  2:584015/151‑1 (MQ=255)                                                                                                                         ggCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCt                    <  1:678963/151‑1 (MQ=255)                                                                                                                          gCAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGTGAACTGGATAACCAGCTa                   <  1:476445/151‑1 (MQ=255)                                                                                                                           cAAGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTaa                  <  2:521259/151‑1 (MQ=255)                                                                                                                            aaGTCCGCGCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTaaa                 <  1:4501/151‑1 (MQ=255)                                                                                                                                 cgcgCTGATGTGCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGtt            >  1:242287/1‑151 (MQ=255)                                                                                                                                   tgcTGATGTGCGACGTTCTAACGGGTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTTCAGCACAACTTCCGATCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACAGGATAACCAGCTAAACAGTTc           >  1:211368/2‑150 (MQ=255)                                                                                                                                           tgCGACGTTCTAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTg  >  2:686738/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                             GCGAGCATCGCGCCTTCGCCACCCTGCGAGACACTGCCTGCGCCATCAGCGACAAATACCGACAATAATGGCTGCTGGTCATTTAACCAGGCAATCTGCATCTGGCAGGCATCCTGACAAGGCAAGTCCGCGCTGATGTGCGACGTTCCAACGGTTGATGCATAGACCAGACGCCAGCTCACACTGACGTCCAGCCTTTTGGCGCTTCCAGCACAACTTCCGTTCCCGGCGTGGAGCGGGAAACCGAACGCAGCGAACTGGATAACCAGCTAAACAGTTCACGGAATTG  >  NC_000913/2152291‑2152579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑