| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,182,939 | T→C | D238G (GAC→GGC) | yegX ← | putative family 25 glycosyl hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,182,939 | 0 | T | C | 98.2% | 178.9 / NA | 57 | D238G (GAC→GGC) | yegX | putative family 25 glycosyl hydrolase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (23/33); minor base A (1/0); total (24/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCTGCCGGGGAGTCTGGTGGGAGTATATCACTGCTTCTTATTTAAGGCGTTTCTTTAATCCCATCAACGAATGCCTGCAACTCCTCCACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGTCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCATTGAAATAGCCCGCCAGATTGGTGTGATAAAAAACGGCTCCTGAGTAAATAATCGGCTTTTT > NC_000913/2182792‑2183084 | ccTGCCGGGGAGTCTGGTGGGAGTATATCACTGCTTCTTATTTAAGGCGTTTCTTTAATCCCATCAACGAATGCCTGCAACTCCTCCACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCg < 1:798269/150‑1 (MQ=255) gCCGGGGAGTCTGGTGGGAGTATATCACTGCTTCTTATTTAAGGCGTTTCTTTAATCCCATCAACGAATGCCTGCAACTCCTCCACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGa < 2:151461/149‑1 (MQ=255) ccGGGGAGTCTGGTGGGAGTATATCACTGCTTCTTATTTAAGGCGTTTCTTTAATCCCATCAACGAATGCCTGCAACTCCTCCACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAAtg < 1:530177/151‑1 (MQ=255) tggttgaagaagatCACTGCTTCTTAGTAAAGGCGTTTCATTTATCCCATCACCGAACGCCCGCAGCTCCTCCACCGTGCCATTAAATTCATTAAAATCCTCCCGACCATTAATCCCATCTGCCTCTCCACTGCCGGAATGTTGTCat < 1:462261/136‑2 (MQ=255) tggtggGAGTATATCACTGCTTCTTATTTAAGGCGTTTCTTTAATCCCATCAACGAATGCCTGCAACTCCTCCACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGACGGAATGCTGCCAGa > 1:89847/1‑149 (MQ=255) tataTCACTGCTTCTTATTTAAGGCGTTTCTTTAATCCCATCAACGAATGCCTGCAACTCCTCCACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGcc > 2:584443/1‑151 (MQ=255) atCACTGCTTCTTATTTAAGGCGTTTCTTTAATCCCATCAACGAATGCCTGCAACTCCTCCACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCAt < 1:323275/150‑1 (MQ=255) gCTTCTTATTTAAGGCGTTTCTTTAATCCCATCAACGAATGCCTGCAACTCCTCCACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGt < 2:414349/149‑1 (MQ=255) gCTTCTTATTTAAGGCGTTTCTTTAATCCCATCAACGAATGCCTGCAACTCCTCCACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCa < 1:365298/151‑1 (MQ=255) tttAAGGCGTTTCTTTAATCCCATCAACGAATGCCTGCAACTCCTCCACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCg > 2:267260/1‑150 (MQ=255) ttAAGGCGTTTCTTTAATCCCATCAACGAATGCCTGCAACTCCTCCACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCgg > 1:187541/1‑150 (MQ=255) tAAGGCGTTTCTTTAATCCCATCAACGAATGCCTGCAACTCCTCCACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGcc > 2:610514/1‑132 (MQ=255) tAAGGCGTTTCTTTAATCCCATCAACGAATGCCTGCAACTCCTCCACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGcc < 1:610474/132‑1 (MQ=255) gCGTTTCTTTAATCCCATCAACGAATGCCTGCAACTCCTCCACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGacgacg > 1:180615/1‑151 (MQ=255) cTTTAATCCCATCAACGAATGCCTGCAACTCCTCCACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGAt > 2:628263/1‑150 (MQ=255) ttAATCCCATCAACGAATGCCTGCAACTCCTCCACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGAtag < 1:48004/150‑1 (MQ=255) ttAATCCCATCAACGAATGCCTGCAACTCCTCCACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGAt < 2:542976/148‑1 (MQ=255) ttAATCCCATCAACGAATGCCTGCAACTCCTCCACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGAt < 1:826365/148‑1 (MQ=255) aaTCCCATCAACGAATGCCTGCAACTCCTCCACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGAtagtag < 1:781913/151‑1 (MQ=255) tCCCATCAACGAATGCCTGCAACTCCTCCACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTg > 1:126644/1‑151 (MQ=255) cccATCAACGAATGCCTGCAACTCCTCCACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTg < 2:849580/150‑1 (MQ=255) ccATCAACGAATGCCTGCAACTCCTCCACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTg > 2:532318/1‑149 (MQ=255) cAACGAATGCCTGCAACTCCTCCCCCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGcca < 1:640879/150‑1 (MQ=255) aaCGAATGCCTGCAACTCCTCCACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGccacc > 1:508005/1‑151 (MQ=255) gAATGCCTGCAACTCCTCCACCGTGCCATTAAATTCATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGccacccac > 2:323845/1‑151 (MQ=255) gAATGCCTGCAACTCCTCCACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGccacccac > 2:359479/1‑151 (MQ=255) aaCTCCTCCACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATAtt < 2:88809/151‑1 (MQ=255) tcctccACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCAt > 2:114021/1‑151 (MQ=255) cctccACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCa < 2:638657/149‑1 (MQ=255) cACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCATTg > 1:3792/1‑148 (MQ=255) aCCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGAta > 2:540230/1‑116 (MQ=255) aCCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGAta < 1:540193/116‑1 (MQ=255) aCCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCATTGAAAt > 1:172160/1‑151 (MQ=255) gTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCATTGAAATAGc > 2:345404/1‑151 (MQ=255) tAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCATTGAAATAGCCCGCCa < 2:688151/150‑1 (MQ=255) aaaTACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCATTGAAATAGCCCGCCAg < 2:726242/150‑1 (MQ=255) aaTACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCATTGAAATATCCCGcc > 2:277315/1‑147 (MQ=255) aTACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCATTGAAATAGCCCGCCAGAtt < 1:779638/151‑1 (MQ=255) aaaaaTCCACCGGACCATTGATGACATCTACCTGTCCACGGCTGACATCCTGCCAGAATCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGAGAGCCACCCCCCACGGATATTCATTGAAATAGCCCGCCAGCTTGGtgtg > 1:265695/2‑151 (MQ=255) aaTCCACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCATTGAAATAGCCCGCCAGATTGGTGTGATa < 2:805506/151‑1 (MQ=255) ccACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCATTGAAATAGCCCGCCAGATTGGTGTGATaaaa < 1:616834/151‑1 (MQ=255) cACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACTTTGATAGTAGTGAGCCACCCACCACGGATATTCATTGAAATAGCCCGCCAGATTGGTGTGATaaaaa < 1:687727/151‑1 (MQ=255) cACCGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCATTGAAATAGCCCGCCAGATTGGTGTGATaaaaa < 2:136863/151‑1 (MQ=255) ccGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCTCCCACCACGGATATTCATTGAAATAGCCCGCCAGATTGGTGTGATAAAAAAc < 2:615737/151‑1 (MQ=255) ccGGACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCATTGAAATAGCCCGCCAGATTGGTGTGATAAAAAAc < 1:767432/151‑1 (MQ=255) ggACCATTGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCATTGAAATAGCCCGCCAGATTGGTGTGATAAAAAACgg < 2:665586/151‑1 (MQ=255) tGATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCATTGAAATAGCCCGCCAGATTGGTGTGATAAAAAACGGCTCCTg > 1:381867/1‑150 (MQ=255) gATGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCATTGAAATAGCCCGCCAGATTGGTGTGATAAAAAACGGCTCCTGa < 1:525194/150‑1 (MQ=255) aTGCCATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCATTGAAATAGCCCGCCAGATTGGTGTGATAAAAAACGGCTCCTGAGt > 1:259619/1‑151 (MQ=255) ccATCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCATTGAAATAGCCCGCCAGATTGGTGTGATAAAAAACGGCTCCTGAGTaa > 2:779773/1‑150 (MQ=255) aTCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCATTGAAATAGCCCGCCAGATTGGTGTGATAAAAAACGGCTCCTGAGTAaat > 2:475196/1‑150 (MQ=255) tCTACCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCATTGAAATAGCCCGCCAGATTGGTGTGATAAAAAACGGCTCCTGAGTAaata > 2:492975/1‑150 (MQ=255) aCCTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCATTGAAATAGCCCGCCAGATTGGTGTGATAAAAAACGGCTCCTGAGTAaataat < 2:244702/149‑1 (MQ=255) ccTGTCCACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCATTGAAATAGCCCGCCAGATTGGTGTGATAAAAAACGGCTCCTGAGTAAATAATCg < 1:45548/150‑1 (MQ=255) ccACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCATTGAAATAGCCCGCCAGATTGGTGTGATAAAAAACGGCTCCTGAGTAAATAATCGGCtttt < 1:703264/151‑1 (MQ=255) ccACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCATTGAAATAGCCCGCCAGATTGGTGTGATAAAAAACGGCTCCTGAGTAAATAATCGGCttt < 2:73780/150‑1 (MQ=255) cACGGCCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCATTGAAATAGCCCGCCAGATTGGTGTGATAAAAAACGGCTCCTGAGTAAATAATCGGCttttt < 1:226698/151‑1 (MQ=255) | CCTGCCGGGGAGTCTGGTGGGAGTATATCACTGCTTCTTATTTAAGGCGTTTCTTTAATCCCATCAACGAATGCCTGCAACTCCTCCACCGTGCCATTAAATACATTAAAATCCACCGGACCATTGATGCCATCTACCTGTCCACGGTCGGAATGCTGCCAGAAGCGCCAGGCCATTCCGTCATTGTCCGGACGACGTTGATAGTAGTGAGCCACCCACCACGGATATTCATTGAAATAGCCCGCCAGATTGGTGTGATAAAAAACGGCTCCTGAGTAAATAATCGGCTTTTT > NC_000913/2182792‑2183084 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |