Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,209,276	G→A	pseudogene (177/2001 nt)	yehQ →	pseudogene

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,209,276	0	G	A	100.0%	205.8 / NA	55	pseudogene (177/2001 nt)	yehQ	pseudogene
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/31);  total (24/31)

GACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAAT  >  NC_000913/2209132‑2209422                                                                                                                                                 |                                                                                                                                                   gACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGa                                                                                                                                                <  1:540286/149‑1 (MQ=255) gACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAAc                                                                                                                                              <  1:462743/151‑1 (MQ=255)  acacCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACg                                                                                                                                             >  1:46810/1‑151 (MQ=255)     ccGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGcc                                                                                                                                          <  2:409421/151‑1 (MQ=255)     ccGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGc                                                                                                                                           <  1:723174/150‑1 (MQ=255)       gCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGcc                                                                                                                                          >  2:525132/1‑149 (MQ=255)       gCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGcc                                                                                                                                          >  1:155413/1‑149 (MQ=255)             ccTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTg                                                                                                                                  <  2:43252/151‑1 (MQ=255)                gACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGa                                                                                                                                 <  2:41718/149‑1 (MQ=255)                   ggCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAagag                                                                                                                            <  2:566525/151‑1 (MQ=255)                          aGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGt                                                                                                                     <  1:128300/151‑1 (MQ=255)                                 ccGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCATCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTg                                                                                                              <  1:779175/151‑1 (MQ=255)                                    gTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCgg                                                                                                           <  1:56714/151‑1 (MQ=255)                                     ttttGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCggg                                                                                                          >  2:639840/1‑151 (MQ=255)                                     ttttGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCgg                                                                                                           >  1:232673/1‑150 (MQ=255)                                     ttttGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCgg                                                                                                           <  2:193795/150‑1 (MQ=255)                                       ttGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGc                                                                                                        >  1:171551/1‑151 (MQ=255)                                          ttAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCAGTACCCAGCTAGCGAACGGCCAGGCACTGAACGAGGCTCAGTGCAGTTGCGGGGcc                                                                                                       >  1:243701/1‑149 (MQ=255)                                                gcAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTAt                                                                                               >  1:157279/1‑151 (MQ=255)                                                gcAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTAt                                                                                               <  2:312837/151‑1 (MQ=255)                                                 cAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTAtg                                                                                              <  2:31564/151‑1 (MQ=255)                                                 cAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTAtg                                                                                              >  1:553945/1‑151 (MQ=255)                                                 cAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTAtg                                                                                              >  1:392298/1‑151 (MQ=255)                                                       tAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGc                                                                                           <  1:460976/148‑1 (MQ=255)                                                        aaGGAACTGGAAAACGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGACCGTACCCAGCTAGCGATCGGCGAGGCACTGAAAGAGGTTCAGTGCAGTTACGGGGACAACGGTATGTGCCGta                                                                                       <  1:271359/151‑2 (MQ=255)                                                         aGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTc                                                                                       >  1:411236/1‑150 (MQ=255)                                                           gAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCATCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATc                                                                                    <  1:544932/151‑1 (MQ=255)                                                            aaCTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATc                                                                                    <  1:69051/150‑1 (MQ=255)                                                             aCTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATcgc                                                                                  >  2:387788/1‑151 (MQ=255)                                                              cTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGCATGTGCCGTCATcgcg                                                                                 >  1:233518/1‑151 (MQ=255)                                                                ggAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTg                                                                               >  1:763006/1‑151 (MQ=255)                                                                  aaaaTGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGAt                                                                             >  2:492542/1‑151 (MQ=255)                                                                   aaaTGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATg                                                                            <  1:12069/151‑1 (MQ=255)                                                                      tGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTg                                                                         >  2:713279/1‑151 (MQ=255)                                                                       ggCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTgg                                                                        <  1:273665/151‑1 (MQ=255)                                                                          aaCGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGgtgt                                                                     >  2:115447/1‑151 (MQ=255)                                                                             gTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTaa                                                                  <  1:653116/151‑1 (MQ=255)                                                                                        cAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACg                                                        <  2:46832/150‑1 (MQ=255)                                                                                        cAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGa                                                       <  2:275090/151‑1 (MQ=255)                                                                                          gCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACt                                                     <  1:234811/151‑1 (MQ=255)                                                                                             aTGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTtg                                                  <  1:133987/151‑1 (MQ=255)                                                                                             aTGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTtg                                                  >  1:137441/1‑151 (MQ=255)                                                                                             aTGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTtg                                                  <  2:490611/151‑1 (MQ=255)                                                                                               gagaACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTtgt                                                 <  1:55218/150‑1 (MQ=255)                                                                                                    cgacgCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGccacc                                           >  2:71739/1‑151 (MQ=255)                                                                                                      acgCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGTAGCCAACGGTATTTGCCGTCATCGCGTAATGCTGGTGTTAAGTTATCAACGACTTTGTGccattac                                         <  1:809668/151‑5 (MQ=255)                                                                                                      acgCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCcaccac                                         <  1:402338/151‑1 (MQ=255)                                                                                                                cGCCACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACg                               >  1:13139/1‑151 (MQ=255)                                                                                                                  ccACCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACgg                              >  1:315980/1‑150 (MQ=255)                                                                                                                    aCCTTCAGTGACGGTGTCCGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACGGaaa                           <  1:367187/151‑1 (MQ=255)                                                                                                                                     ccGTACCCAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGAt          >  1:221596/1‑151 (MQ=255)                                                                                                                                          cccAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGc     >  2:733626/1‑151 (MQ=255)                                                                                                                                           ccAGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCa    <  1:764153/151‑1 (MQ=255)                                                                                                                                             aGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAAt  <  1:669051/151‑1 (MQ=255)                                                                                                                                             aGCTAGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAAt  >  2:80510/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                   GACACCGCAGGCCCTGACGGCGTTAAGCAATGCCGGTTTTGTTAAGCGCAGTCTTAAGGAACTGGAAAATGGCAACGTCCCGGAGATCAGCCATGAGAACGACGCTTTAATCGCCACCTTCAGTGACGGTGTCCGTACCCAGCTGGCGAACGGCCAGGCACTGAAAGAGGCTCAGTGCAGTTGCGGGGCCAACGGTATGTGCCGTCATCGCGTGATGCTGGTGTTAAGTTATCAACGACTTTGTGCCACCACTCAGTCTACGGAAAAAGAAGAAGAGTGGGATCCGGCAAT  >  NC_000913/2209132‑2209422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑