Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,209,868	A→G	pseudogene (769/2001 nt)	yehQ →	pseudogene

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,209,868	0	A	G	100.0%	181.7 / NA	53	pseudogene (769/2001 nt)	yehQ	pseudogene
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/35);  total (18/35)

ATCATCTGACGATCCGTTTGCCAGCGAAGAAGGCAACGCGTGTCGTCAATATGTTCAGCAATTAAGCCAGACATTATGGCTTGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGACCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAG  >  NC_000913/2209720‑2210012                                                                                                                                                     |                                                                                                                                                 atcatcTGACGATCCGTTTGCCAGCGAAGAAGGCAACGCGTGTCGTCAATATGTTCAGCAATTAAGCCAGACATTATGGCTTGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGcc                                                                                                                                                <  2:553982/151‑1 (MQ=255)   catcTGACGATCCGTTTGCCAGCGAAGAAGGCAACGCGTGTCGTCAATATGTTCAGCAATTAAGCCAGACATTATGGCTTGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCt                                                                                                                                               <  2:831008/150‑1 (MQ=255)       tGACGATCCGTTTGCCAGCGAAGAAGGCAACGCGTGTCGTCAATATGTTCAGCAATTAAGCCAGACATTATGGCTTGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCaa                                                                                                                                           >  2:302810/1‑150 (MQ=255)        gACGATCCGTTTGCCAGCGAAGAAGGCAACGCGTGTCGTCAATATGTTCAGCAATTAAGCCAGACATTATGGCTTGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCaa                                                                                                                                           <  2:148445/149‑1 (MQ=255)        gACGATCCGTTTGCCAGCGAAGAAGGCAACGCGTGTCGTCAATATGTTCAGCAATTAAGCCAGACATTATGGCTTGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCa                                                                                                                                            <  2:677697/148‑1 (MQ=255)        gACGATCCGTTTGCCAGCGAAGAAGGCAACGCGTGTCGTCAATATGTTCAGCAATTAAGCCAGACATTATGGCTTGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAAc                                                                                                                                          <  2:730695/150‑1 (MQ=255)           gATCCGTTTGCCAGCGAAGAAGGCAACGCGTGTCGTCAATATGTTCAGCAATTAAGCCAGACATTATGGCTTGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTgg                                                                                                                                       >  2:647651/1‑150 (MQ=255)           gATCCGTTTGCCAGCGAAGAAGGCAACGCGTGTCGTCAATATGTTCAGCAATTAAGCCAGACATTATGGCTTGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGgc                                                                                                                                      <  2:392330/151‑1 (MQ=255)                gTTTGCCAGCGAAGAAGGCAACGCGTGTCGTCAATATGTTCAGCAATTAAGCCAGACATTATGGCTTGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTgg                                                                                                                                 >  1:187995/1‑151 (MQ=255)                gTTTGCCAGCGAAGAAGGCAACGCGTGTCGTCAATATGTTCAGCAATTAAGCCAGACATTATGGCTTGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTgg                                                                                                                                 >  1:166830/1‑151 (MQ=255)                          gaagaaGGCAACGCGTGTCGTCAATATGTTCAGCAATTAAGCCAGACATTATGGCTTGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAAt                                                                                                                       <  1:541726/151‑1 (MQ=255)                                ggCAACGCGTGTCGTCAATATGTTCAGCAATTAAGCCAGACATTATGGCTTGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTAc                                                                                                                 <  2:830101/151‑1 (MQ=255)                                   aaCGCGTGTCGTCAATATGTTCAGCAATTAAGCCAGACATTATGGCTTGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGc                                                                                                              <  1:733583/151‑1 (MQ=255)                                    aCGCGTGTCGTCAATATGTTCAGCAATTAAGCCAGACATTATGGCTTGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCa                                                                                                             <  1:75385/151‑1 (MQ=255)                                      gcgTGTCGTCAATATGTTCAGCAATTAAGCCAGACATTATGGCTTGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTTCAGGCGGCAGAGGCCTTCAACTGGCGCTGGGGGAGTGAATCGCTACGGCAAc                                                                                                           >  1:816085/1‑151 (MQ=255)                                       cgTGTCGTCAATATGTTCAGCAATTAAGCCAGACATTATGGCTTGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGGGAGTGAATCGCTACGGCAAc                                                                                                           >  2:825249/1‑150 (MQ=255)                                        gtgtCGTCAATATGTTCAGCAATTAAGCCAGACATTATGGCTTGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTg                                                                                                         <  2:250049/151‑1 (MQ=255)                                          gtcgtcAATATGTTCAGCAATTAAGCCAGACATTATGGCTTGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTgc                                                                                                        >  1:198767/1‑150 (MQ=255)                                            cgtcAATATGTTCAGCAATTAAGCCAGACATTATGGCTTGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCCGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTAAGGCAACTgcgcg                                                                                                     >  2:662531/1‑151 (MQ=255)                                                aaTATGTTCAGCAATTAAGCCAGACATTATGGCTTGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGGGAGTGAATCGCTACGGCAACTGCGCGcc                                                                                                   >  2:702248/1‑149 (MQ=255)                                                    tGTTCAGCAATTAAGCCAGACATTATGGCTTGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGtt                                                                                             >  2:584864/1‑151 (MQ=255)                                                      ttCAGCAATTAAGCCAGACATTATGGCTTGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGGGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTg                                                                                            >  2:796160/1‑150 (MQ=255)                                                                 aGCCAGACATTATGGCTTGGCGGCACCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCAAACGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCAc                                                                                 >  2:183267/1‑150 (MQ=255)                                                                      gACATTATGGCTTGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCcgc                                                                           <  1:450630/151‑1 (MQ=255)                                                                        cATTATGGCTTGGCGGCATCCGCCAGGCGCTCATTCATTACGAGGCAGCATTCAACATCGCATTGGAGGCGGCCGAGGCCTGCGAATGGCGGTGGGTGAGCGGACCGCTTCGGCAACTGCGCGCCAGCGCTGACGCCTTCCACGACcgcg                                                                          >  1:169213/1‑150 (MQ=255)                                                                            aTGGCTTGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCgccag                                                                      <  1:525094/150‑1 (MQ=255)                                                                                  tGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCACCCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACta                                                                <  1:770207/150‑1 (MQ=255)                                                                                   ggcggcATCAGCCAGCCGCTCAGCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCGCta                                                                <  2:243725/149‑1 (MQ=255)                                                                                           cagccagcCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCgg                                                       >  2:444531/1‑150 (MQ=255)                                                                                           cagccagcCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGga                                                      <  1:3266/151‑1 (MQ=255)                                                                                              ccagccGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGaa                                                   <  2:41809/151‑1 (MQ=255)                                                                                               cagccGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAAt                                                  <  1:647609/151‑1 (MQ=255)                                                                                               cagccGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAAt                                                  <  2:411268/151‑1 (MQ=255)                                                                                                 gccGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGc                                                <  2:725378/151‑1 (MQ=255)                                                                                                  ccGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCt                                               <  1:80487/151‑1 (MQ=255)                                                                                                       cATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACAt                                          <  2:763063/151‑1 (MQ=255)                                                                                                        aTCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCTGCGCCAGCCACTATAATGCCGGAGAATGCTTACATc                                         >  1:636714/1‑151 (MQ=255)                                                                                                          ccATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAg                                       <  1:169087/151‑1 (MQ=255)                                                                                                           cATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGc                                      <  2:232697/151‑1 (MQ=255)                                                                                                              tACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCtt                                    <  2:196602/150‑1 (MQ=255)                                                                                                               aCGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTg                                   <  1:329948/150‑1 (MQ=255)                                                                                                                     cagcaTTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCAtt                            <  1:482037/151‑1 (MQ=255)                                                                                                                       gcaTTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTaa                          <  2:377301/151‑1 (MQ=255)                                                                                                                       gcaTTCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTaa                          <  2:292380/151‑1 (MQ=255)                                                                                                                          ttCAACCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTa                           <  1:761126/147‑1 (MQ=255)                                                                                                                              aCCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTc                    <  2:511839/150‑1 (MQ=255)                                                                                                                              aCCGCGCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCg                   <  2:350461/151‑1 (MQ=255)                                                                                                                                cgcgCATTGCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGAt                 >  2:326492/1‑151 (MQ=255)                                                                                                                                        gCAGGCGGCAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCCGCTTGCGGCATTAAACAGTCGATTAAATTgc         >  2:801769/1‑151 (MQ=255)                                                                                                                                             cggcAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGcac     >  1:207894/1‑150 (MQ=255)                                                                                                                                              ggcAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACaa   <  1:806841/151‑1 (MQ=255)                                                                                                                                               gcAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAag  <  2:484585/151‑1 (MQ=255)                                                                                                                                               gcAGAGGCCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAag  <  1:651431/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 ATCATCTGACGATCCGTTTGCCAGCGAAGAAGGCAACGCGTGTCGTCAATATGTTCAGCAATTAAGCCAGACATTATGGCTTGGCGGCATCAGCCAGCCGCTCATCCATTACGAGGCAGCATTCAACCGCGCATTGCAGGCGGCAGAGACCTGCAACTGGCGCTGGGTGAGTGAATCGCTACGGCAACTGCGCGCCAGCGTTGATGCCTTCCACGCCCGCGCCAGCCACTATAATGCCGGAGAATGCTTACATCAGCTTGCGGCATTAAACAGTCGATTAAATTGCGCACAAG  >  NC_000913/2209720‑2210012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑