Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,236,037	G→A	G258D (GGT→GAT)	preA →	dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,236,037	0	G	A	100.0%	203.1 / NA	52	G258D (GGT→GAT)	preA	dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/24);  total (28/24)

CGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAGCGGGTTGTCGCATTATCTCGCCGAT  >  NC_000913/2235891‑2236182                                                                                                                                                   |                                                                                                                                                  cGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATc                                                                                                                                               >  1:749752/1‑151 (MQ=255)     cTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATcggcg                                                                                                                                           >  2:783088/1‑151 (MQ=255)                     gTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGgga                                                                                                                           <  2:167962/151‑1 (MQ=255)                                gTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTg                                                                                                                >  2:782070/1‑151 (MQ=255)                                 ttAACGGAAAATCGCGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTg                                                                                                                <  2:544413/150‑1 (MQ=255)                                 ttAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTg                                                                                                                >  2:329694/1‑150 (MQ=255)                                  tAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTg                                                                                                                >  1:308027/1‑149 (MQ=255)                                     cGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGttt                                                                                                           >  1:100684/1‑151 (MQ=255)                                      ggAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGtttt                                                                                                          >  1:510982/1‑151 (MQ=255)                                       gAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGttttt                                                                                                         <  1:11421/151‑1 (MQ=255)                                                 tATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGgc                                                                                               >  2:177069/1‑151 (MQ=255)                                                 tATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGgc                                                                                               >  1:731532/1‑151 (MQ=255)                                                     tCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGgcgc                                                                                             >  2:538877/1‑149 (MQ=255)                                                     tCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGgcgc                                                                                             >  2:818614/1‑149 (MQ=255)                                                        ggATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCgcagca                                                                                         >  2:108647/1‑150 (MQ=255)                                                         gATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAAc                                                                                       <  1:801709/151‑1 (MQ=255)                                                          atatTCCGTTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTTGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCaa                                                                                        <  2:171069/149‑1 (MQ=255)                                                          atatTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACg                                                                                      <  2:620855/151‑1 (MQ=255)                                                          atatTACGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACg                                                                                      >  1:114912/1‑151 (MQ=255)                                                                 ggTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAgg                                                                               >  2:516037/1‑151 (MQ=255)                                                                     aaGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGac                                                                           >  2:14022/1‑151 (MQ=255)                                                                      aGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGacc                                                                          >  2:546484/1‑151 (MQ=255)                                                                      aGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAAAGTTACAGGTGacc                                                                          >  2:153276/1‑151 (MQ=255)                                                                             aaaCCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGc                                                                    >  2:557160/1‑150 (MQ=255)                                                                             aaaCCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGc                                                                    >  1:381326/1‑150 (MQ=255)                                                                                 cGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGcatcat                                                               >  1:819893/1‑151 (MQ=255)                                                                                 cGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGcatcat                                                               <  1:585122/151‑1 (MQ=255)                                                                                     cGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAg                                                           <  2:749809/151‑1 (MQ=255)                                                                                                  attcaGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCgg                                               >  2:520918/1‑150 (MQ=255)                                                                                                    tcaGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGAt                                             <  2:13243/150‑1 (MQ=255)                                                                                                      aGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGt                                          <  1:495717/151‑1 (MQ=255)                                                                                                       gCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTg                                         >  1:283063/1‑151 (MQ=255)                                                                                                         aaaTGCGCACCCATCCAGAACTTCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTgta                                       <  1:859404/151‑3 (MQ=255)                                                                                                           aTGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTgg                                        >  1:170925/1‑148 (MQ=255)                                                                                                           aTGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAg                                     <  1:675260/151‑1 (MQ=255)                                                                                                             gcgcACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGa                                       >  1:73709/1‑147 (MQ=255)                                                                                                             gcgcACCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGa                                    <  2:611749/150‑1 (MQ=255)                                                                                                                 aCCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATgg                               >  2:356078/1‑151 (MQ=255)                                                                                                                 aCCCATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATgg                               >  1:806284/1‑151 (MQ=255)                                                                                                                  cccATCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGc                              <  2:731575/151‑1 (MQ=255)                                                                                                                     aTCCAGAACTGCGCGATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCGCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCaa                            <  1:311322/150‑1 (MQ=255)                                                                                                                                 gcgATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAGCGGGTTGTCGCa               <  1:565650/151‑1 (MQ=255)                                                                                                                                  cgATTTCCCAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAGCGGGTTGTCGCAt              >  1:150503/1‑151 (MQ=255)                                                                                                                                          cAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCATTACGGGTATCGGATAGTGGAAGATATGGCAAGCGGGTTGTCGCATTAtctc        <  1:459362/149‑1 (MQ=255)                                                                                                                                          cAATCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAGCGGGTTGTCGCATTATCTCGc      <  2:68764/151‑1 (MQ=255)                                                                                                                                           aaTCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAGCGGGTTGTCGCATTATCTCGcc     >  2:153935/1‑151 (MQ=255)                                                                                                                                           aaTCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAGCAGGTTGTCGCATTATCTCGcc     <  2:299363/151‑1 (MQ=255)                                                                                                                                            aTCAGCGATATCGGCGGCATTGAAGCCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCAGCATGCAGTACGGGTATCGGATGGTGGAAGATATGGCAAGCGGGTTGTCGCATTATCTCGCCg    <  2:690533/151‑1 (MQ=255)                                                                                                                                            aTCAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAGCGGGTTGTCGCATTATCTCGCCg    <  2:834841/151‑1 (MQ=255)                                                                                                                                              cAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAGCGGGTTGTCGCATTATCTCGCCGa   <  2:322485/150‑1 (MQ=255)                                                                                                                                              cAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAGCGGGTTGTCGCATTATCTCGCCGAt  <  1:654840/151‑1 (MQ=255)                                                                                                                                              cAGCGATATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAGCGGGTTGTCGCATTATCTCGCCGAt  <  2:823314/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  CGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGAAACCTGGGAGGATGCGGCTGAGTTTTTATTGCTCGGCGCAGCAACGTTACAGGTGACCACCGGCATCATGCAGTACGGGTATCGGATAGTGGAAGATATGGCAAGCGGGTTGTCGCATTATCTCGCCGAT  >  NC_000913/2235891‑2236182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑