Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,243,790	T→C	intergenic (‑138/‑120)	folE ← / → yeiG	GTP cyclohydrolase I/S‑formylglutathione hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,243,790	0	T	C	100.0%	180.9 / NA	53	intergenic (‑138/‑120)	folE/yeiG	GTP cyclohydrolase I/S‑formylglutathione hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/20);  total (33/20)

GATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCTGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGCATGGAAATGCTCGAAGAGCACCGCTGTT  >  NC_000913/2243645‑2243937                                                                                                                                                  |                                                                                                                                                    gATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCt                                                                                                                                                 <  1:574551/150‑1 (MQ=255)    ggCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTtc                                                                                                                                              <  1:583164/150‑1 (MQ=255)      cATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTtcatc                                                                                                                                           <  2:613903/151‑1 (MQ=255)      cATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTtcatc                                                                                                                                           >  1:316858/1‑151 (MQ=255)        tttATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTtcatc                                                                                                                                           >  2:523874/1‑149 (MQ=255)        tttATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTtcatc                                                                                                                                           >  1:287492/1‑149 (MQ=255)         ttATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATcagc                                                                                                                                        >  2:294840/1‑151 (MQ=255)         ttATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATcag                                                                                                                                         >  2:355484/1‑150 (MQ=255)              aTTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCatatt                                                                                                                                   <  1:693553/151‑2 (MQ=255)                    ccTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTaa                                                                                                                               <  1:472529/149‑1 (MQ=255)                           gtgtGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGtt                                                                                                                      >  1:467561/1‑151 (MQ=255)                             gtgACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTAt                                                                                                                    >  1:199185/1‑151 (MQ=255)                               gACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGc                                                                                                                  >  2:97922/1‑151 (MQ=255)                                  gCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCaa                                                                                                                >  2:259423/1‑150 (MQ=255)                                          ccTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGaa                                                                                                        <  1:473588/150‑1 (MQ=255)                                              cGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGa                                                                                                    >  1:207901/1‑150 (MQ=255)                                               gTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGaaa                                                                                                  <  2:500224/151‑1 (MQ=255)                                                  ggggCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGtt                                                                                               <  1:850393/151‑1 (MQ=255)                                                      cAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCTAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACt                                                                                            >  1:784076/1‑150 (MQ=255)                                                                aaGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACAtt                                                                                 >  2:578976/1‑151 (MQ=255)                                                                 aGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATATTTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTg                                                                                >  2:229311/1‑151 (MQ=255)                                                                 aGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTg                                                                                >  2:857874/1‑151 (MQ=255)                                                                  gCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGa                                                                               <  1:692501/151‑1 (MQ=255)                                                                            aGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGt                                                                      >  2:796504/1‑150 (MQ=255)                                                                            aGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTa                                                                     <  1:233178/151‑1 (MQ=255)                                                                              ccTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTatg                                                                   <  2:311647/151‑1 (MQ=255)                                                                                tGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTatgat                                                                 >  2:648678/1‑151 (MQ=255)                                                                                 gATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTatgatg                                                                >  2:129318/1‑151 (MQ=255)                                                                                     attGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATgaaga                                                            <  2:262678/151‑1 (MQ=255)                                                                                          gcatGAGGCGGCGCACAGAATCCAGTATTCTCAGGGCTCCTGATTGTAATTGCCGCCGACTTTCATAAGCAGATGTTCAAACATCGTTATGCAAATACGGAAGGGAAAGTTACTCACAGCACATTGACTAGACGGGATGATGAAGAAAGTg                                                       >  1:666628/4‑151 (MQ=255)                                                                                          gCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAAtt                                                        <  1:555122/150‑1 (MQ=255)                                                                                          gCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTg                                                       <  2:142676/151‑1 (MQ=255)                                                                                           cGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGc                                                      >  2:660085/1‑151 (MQ=255)                                                                                              gAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCaa                                                    >  2:365603/1‑150 (MQ=255)                                                                                                ggcggcGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAATCGGTATGATGAAGAAATTGCAaaca                                                 <  1:12601/151‑1 (MQ=255)                                                                                                 gcggcgCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAaaca                                                 >  1:655113/1‑150 (MQ=255)                                                                                                   ggcgCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAacaaca                                              <  1:98354/151‑1 (MQ=255)                                                                                                     cgcACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAacaacaca                                            <  2:11365/151‑1 (MQ=255)                                                                                                        acaTAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAacaacacaac                                          >  1:259064/1‑150 (MQ=255)                                                                                                        acaTAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAAcaacacaaca                                         <  2:244413/151‑1 (MQ=255)                                                                                                          aTAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAAcaacacaaca                                         >  2:333743/1‑149 (MQ=255)                                                                                                            aaTCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAgg                                      >  2:855061/1‑150 (MQ=255)                                                                                                            aaTCCAGTATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAgg                                      <  2:431800/150‑1 (MQ=255)                                                                                                                   tATTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGc                              >  1:386399/1‑151 (MQ=255)                                                                                                                    aTTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGc                              >  1:712417/1‑150 (MQ=255)                                                                                                                    aTTCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGc                              >  2:644121/1‑150 (MQ=255)                                                                                                                      tCACAGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGCATg                           >  2:339743/1‑151 (MQ=255)                                                                                                                       cacaGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCCCGCATg                           <  2:756109/150‑1 (MQ=255)                                                                                                                       cacaGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGCATgg                          >  2:801114/1‑151 (MQ=255)                                                                                                                         caGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGCATGGa                         >  1:281604/1‑150 (MQ=255)                                                                                                                          aGCGATAATTATTGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGCATGGaa                        >  1:167541/1‑150 (MQ=255)                                                                                                                                   tattGTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGCATGGAAATGCTCGa                >  2:478732/1‑149 (MQ=255)                                                                                                                                       gTAATTGCCGCCGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGCATGGAAATGCTCGAAGAGc           >  2:336060/1‑150 (MQ=255)                                                                                                                                             gccgccgccTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGCATGGAAATGCTCGAAGAGCACCGCTg    >  1:826195/1‑151 (MQ=255)                                                                                                                                               cgccgcCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGCATGGAAATGCTCGAAGAGCACCGCTGtt  <  2:264164/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    GATGGCATTTATGATTTCTCCTGCAGGTGTGACGCCTCCGCCCTGCGTGGGGGCAAAGTTATTAAGCTGATTTACAGCCTGATTATTGTGCGTGAGGCGGCGCACATAATCCAGTATTCACAGCGATAATTATTGTAATTGCCGCTGCCTTTCATCAGCAGATGTTAAAACATCGTTATGCAAATACGGAAGTGAAAGTTACTCACAGCACATTGAATAAACGGTATGATGAAGAAATTGCAAACAACACAACAAGGAGCCACGCATGGAAATGCTCGAAGAGCACCGCTGTT  >  NC_000913/2243645‑2243937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑