Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	308,755	C→T	S193N (AGC→AAC)	ecpC ←	ECP production outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	308,755	0	C	T	100.0%	135.2 / NA	39	S193N (AGC→AAC)	ecpC	ECP production outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/14);  total (25/14)

CCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGTGCCCAGCGCTTCGCGCTTGAC  >  NC_000913/308608‑308900                                                                                                                                                    |                                                                                                                                                  ccGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTg                                                                                                                                                 <  1:40765/150‑1 (MQ=255)       aaTCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGACGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGAtgtg                                                                                                                                           >  2:27429/1‑150 (MQ=255)        aTCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGtt                                                                                                                                         >  1:755723/1‑151 (MQ=255)          cGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTg                                                                                                                                        <  2:537822/150‑1 (MQ=255)           ggTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCt                                                                                                                                      <  2:276813/151‑1 (MQ=255)            gTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTc                                                                                                                                     >  1:240193/1‑151 (MQ=255)               aCCGGTAAAATCGCGTTCATACATCGCTTTATTTAAATCACTTTCCTCTCGACTGCTACCGAGCCCATGCAGCTAGCCGTCGAGCCTCACATGATGTTCGCGCAATCCAGTAACGTTATTCATCGACAGATAATTGGTTGTGTTGCTccc                                                                                                                                   <  1:700118/150‑1 (MQ=255)                ccGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCccgc                                                                                                                                 <  2:818838/151‑1 (MQ=255)                  ggaaaaaTCGCTTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGGGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATATTTGGATGTGTTGCTCccgccg                                                                                                                               <  1:405405/148‑1 (MQ=255)                  ggCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCATATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCccgccg                                                                                                                               >  2:255430/1‑151 (MQ=255)                                cATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGgtt                                                                                                                 >  1:38694/1‑151 (MQ=255)                                    cATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGgttgtt                                                                                                              >  2:849530/1‑150 (MQ=255)                                       cGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTtat                                                                                                            >  2:790609/1‑149 (MQ=255)                                        gCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTtat                                                                                                            >  2:485302/1‑148 (MQ=255)                                         cTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGAc                                                                                                        >  1:375017/1‑151 (MQ=255)                                              tataATTCACTGTCCTGTTGACCGCTACCGCTCCCGTACAGCGAGCCGTCGAGCACAACATGATGTTCGCGCAGGGCAGAAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGccc                                                                                                    >  2:586025/1‑150 (MQ=255)                                                 aaTTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGt                                                                                                >  2:539787/1‑151 (MQ=255)                                                         gTCCTGTTGCCCGCCCCCCCTCCCTAACGCCGGTCCGCCGAGCACCACATGATGGTCGCGCGTTGCATTAACTTTATTCGGCGACAGATGGTGGGTTGTGTTCCCCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGc                                                                                          <  1:64519/149‑1 (MQ=255)                                                           cctgtggcccccccccGACCCCGTACAGCGGGCCGCCGACCCCCACATGTTGTCCGCGCAGTGCAGAAACTTTTTTCAGCGAAAGATAGTGGGTTGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCt                                                                                         <  2:69467/134‑1 (MQ=255)                                                             tGTTGCCCGCTCCCGACCCCGAACGGCGGGCGGGCGAGCCCCACTTGATGTGCGCGCGGGGCAGTAATTTTATTGGGCGACAGATGGTTGGAGTTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAg                                                                                           <  2:713898/144‑1 (MQ=255)                                                                          cGACCCCGTACAGCGGGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACTTTATTCAGCGAAAGATATTTGGATGTTTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGa                                                                           <  2:320233/147‑1 (MQ=255)                                                                                    cAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTg                                                             >  2:119751/1‑151 (MQ=255)                                                                                        gAGCCGTCGACCACCACATGTTGTTCGCGCGGTGCAGTATTGTTATTCAGCAAAAAATAGTGGGATGTTTTCCCCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACt                                                         <  1:175483/151‑1 (MQ=255)                                                                                          gCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGc                                                       >  1:464639/1‑151 (MQ=255)                                                                                               cGAGCACCACTTGTTGTGCGCTAAGTGCAGTAAGTTTTTTGAGGGACAGTTTGTGGGTTGTTTTGCCCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTgg                                                            <  1:843366/141‑1 (MQ=255)                                                                                                    accacATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGACAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCtt                                             >  2:3109/1‑151 (MQ=255)                                                                                                      cacaTGTTGTGCGCGCAGGGCATTAATTTTATTCCGCAACAGAGTGTGGGTTGTTTTGCCCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACt                                                         <  1:651618/137‑1 (MQ=255)                                                                                                              gTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTg                                    >  1:586498/1‑150 (MQ=255)                                                                                                                  gcgcAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCgt                               >  1:657583/1‑151 (MQ=255)                                                                                                                   cgcAGTGCAGTAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCgt                               >  2:632047/1‑150 (MQ=255)                                                                                                                      agtgcagtAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCgtagt                            >  1:554385/1‑150 (MQ=255)                                                                                                                        tgcagtAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACg                         >  2:339301/1‑151 (MQ=255)                                                                                                                             tAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGTGcc                    >  2:191354/1‑151 (MQ=255)                                                                                                                             tAACGTTATTCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGTGcc                    >  2:639810/1‑151 (MQ=255)                                                                                                                               actttATTCAGCGACAGATAGTTGGTTGTGTTCCCCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGt                       <  2:439227/143‑1 (MQ=255)                                                                                                                                     ttCAGCGACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGTGCCCAGCGCtt            >  1:201005/1‑151 (MQ=255)                                                                                                                                           gACAGATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGTGCCCAGCGCTTCGCGCt      >  2:601832/1‑151 (MQ=255)                                                                                                                                               gATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGTGCCCAGCGCTTCGCGCTTGAc  >  1:296412/1‑151 (MQ=255)                                                                                                                                               gATAGTTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGTGCCCAGCGCTTCGCGCTTGAc  >  1:242673/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                  CCGGCAAATCGGTGACCGGCAAAATCGCGTTCATACATCGCTTTATATAATTCACTGTCCTGTTGACCGCTACCGATCCCGTACAGCGAGCCGTCGAGCACCACATGATGTTCGCGCAGTGCAGTAACGTTATTCAGCGACAGATAGCTGGATGTGTTGCTCCCGCCGTTACGCAACTGGTTGTTATAGACGCCCAAGTTATAGCTCAGATTACTGCTGAGGGTGTTAACACTGGACTGCCCGATGTCTTCGCTACGTGAGCGTAGTACGGTGCCCAGCGCTTCGCGCTTGAC  >  NC_000913/308608‑308900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑