Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,251,116 G→A D93N (GAT→AAT)  nfo → endonuclease IV with intrinsic 3'‑5' exonuclease activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,251,1160GA100.0% 194.7 / NA 52D93N (GAT→AAT) nfoendonuclease IV with intrinsic 3'‑5' exonuclease activity
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/24);  total (28/24)

GCTCACGACGCAAACCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAGATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGACAG  >  NC_000913/2250968‑2251263
                                                                                                                                                    |                                                                                                                                                   
gCTCACGACGCAAACCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAAt                                                                                                                                                   <  1:723237/151‑1 (MQ=255)
           aaaCCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCg                                                                                                                                        >  2:480094/1‑151 (MQ=255)
            aaCCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGt                                                                                                                                       >  2:801623/1‑151 (MQ=255)
            aaCCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGt                                                                                                                                       >  2:783112/1‑151 (MQ=255)
             aCCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGtt                                                                                                                                      <  2:78250/151‑1 (MQ=255)
                   gATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAAc                                                                                                                                <  2:583401/151‑1 (MQ=255)
                     tGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAAc                                                                                                                                >  1:816473/1‑149 (MQ=255)
                      gAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGc                                                                                                                             >  2:14786/1‑151 (MQ=255)
                       aaTTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCt                                                                                                                            >  2:156504/1‑151 (MQ=255)
                        aTTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTg                                                                                                                           <  2:181686/151‑1 (MQ=255)
                        aTTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTg                                                                                                                           <  1:509777/151‑1 (MQ=255)
                          tCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTggg                                                                                                                         >  2:839986/1‑151 (MQ=255)
                           cAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTgggg                                                                                                                        >  1:845265/1‑151 (MQ=255)
                             aaGCCGCCTGTGAAAAATATCACTACACATCGGCGCACATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACCTCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGct                                                                                                                      >  1:254698/1‑151 (MQ=255)
                              aGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGctt                                                                                                                     <  2:665019/151‑1 (MQ=255)
                                 cgccTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGctttct                                                                                                                  >  2:155566/1‑151 (MQ=255)
                                        gAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCa                                                                                                           <  2:236947/151‑1 (MQ=255)
                                         aaaaaTATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCa                                                                                                           >  1:196189/1‑150 (MQ=255)
                                           aaaTATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACt                                                                                                        <  2:659282/151‑1 (MQ=255)
                                           aaaTATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACt                                                                                                        >  2:161862/1‑151 (MQ=255)
                                                     acacATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTg                                                                                               <  2:747978/150‑1 (MQ=255)
                                                       acaTCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGc                                                                                             >  1:718579/1‑150 (MQ=255)
                                                       acaTCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCa                                                                                            <  2:626453/151‑1 (MQ=255)
                                                            ggCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCAt                                                                                       >  2:378960/1‑151 (MQ=255)
                                                              cgcAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCt                                                                                     <  2:107493/151‑1 (MQ=255)
                                                                   aTTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGc                                                                                <  2:845324/151‑1 (MQ=255)
                                                                      cttcCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGc                                                                                >  2:554977/1‑148 (MQ=255)
                                                                              cGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCaga                                                                      >  1:276077/1‑150 (MQ=255)
                                                                              cGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCaga                                                                      >  2:642794/1‑150 (MQ=255)
                                                                               gACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCagaag                                                                    <  1:272080/151‑1 (MQ=255)
                                                                                      aTCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTg                                                             >  2:591289/1‑151 (MQ=255)
                                                                                         tGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCt                                                          >  2:380693/1‑151 (MQ=255)
                                                                                            ttAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTgc                                                       >  2:404760/1‑151 (MQ=255)
                                                                                                   cGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTAt                                                 <  2:690369/150‑1 (MQ=255)
                                                                                                     gACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGc                                              <  2:612193/151‑1 (MQ=255)
                                                                                                        aTCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCg                                            >  2:189085/1‑150 (MQ=255)
                                                                                                          ccGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGaa                                          <  2:148337/150‑1 (MQ=255)
                                                                                                          ccGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGaa                                          <  1:273812/150‑1 (MQ=255)
                                                                                                          ccGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGaa                                          <  1:411652/150‑1 (MQ=255)
                                                                                                          ccGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAAt                                         >  2:100733/1‑151 (MQ=255)
                                                                                                             gTCACTGACGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATcc                                       >  1:721685/1‑150 (MQ=255)
                                                                                                              tCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCAt                                     >  1:592960/1‑151 (MQ=255)
                                                                                                                   gAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCATATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAAc                                 <  1:375358/150‑1 (MQ=255)
                                                                                                                   gAAGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAAc                                 <  1:632146/150‑1 (MQ=255)
                                                                                                                    aaGCTCTGGAAAAATCGCGCGATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACAt                               >  1:222459/1‑151 (MQ=255)
                                                                                                                                   cgcgcgATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAAc                <  1:842552/151‑1 (MQ=255)
                                                                                                                                       cgATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCa             >  2:780469/1‑150 (MQ=255)
                                                                                                                                       cgATGCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCa             >  1:268203/1‑150 (MQ=255)
                                                                                                                                           gCCTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGgtg        <  1:238703/151‑1 (MQ=255)
                                                                                                                                            ccTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGgtgt       <  1:26431/151‑1 (MQ=255)
                                                                                                                                            ccTTTATAAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCAACCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGAGCTGGATAAAACTCAAGgtg        >  2:541835/1‑150 (MQ=255)
                                                                                                                                                 ataAATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGACAg  <  1:24008/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCTCACGACGCAAACCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAGATGAAATGCAGCGTTGCGAACAGCTGGGGCTTTCTTTGCTCAACTTCCACCCTGGCAGCCATCTGATGCAGATTTCAGAAGAGGATTGCCTTGCGCGTATTGCCGAATCCATCAACATTGCGCTGGATAAAACTCAAGGTGTGACAG  >  NC_000913/2250968‑2251263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑