| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,265,230 | A→G | G22G (GGT→GGC) | yeiW ← | UPF0153 cysteine cluster protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,265,230 | 0 | A | G | 90.4% | 146.5 / ‑3.7 | 54 | G22G (GGT→GGC) | yeiW | UPF0153 cysteine cluster protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); major base G (22/25); minor base T (5/0); total (29/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.15e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGA > NC_000913/2265086‑2265372 | ctgctgACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCAAGAATACCagg > 2:522936/1‑150 (MQ=255) gctgACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGaa < 2:739822/150‑1 (MQ=255) gctgACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAAt < 1:30221/151‑1 (MQ=255) ttACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCAAGCCGGGGATAGGGCTgg > 2:409176/1‑148 (MQ=255) cGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTg > 2:645112/1‑150 (MQ=255) acaTTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGGATTGGCGGGATTGCCCTCTAGCAACTCGGGGATAGGGCTGGAAATTTAAgg > 1:451180/1‑151 (MQ=255) caTTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAggg < 1:83505/151‑1 (MQ=255) aTTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTTCCATCTGGCATGCCGGGGATAGGGCTGGAAATTTAAgggg > 2:455881/1‑151 (MQ=255) tttCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGCAGTGCACTCTCGCGTTTCGGGGAAAGGGGTGGGAAATGAAaggg > 1:448050/1‑150 (MQ=255) ttCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGc < 2:374631/150‑1 (MQ=255) cAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGt < 1:90270/151‑1 (MQ=255) ggCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAac < 1:519196/149‑1 (MQ=255) gCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGGCCTCTGGCATGCCGGGAATAGGGGTGGAAATTGAAGGGGGAGTGCAacac > 2:60431/1‑150 (MQ=255) cTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCCTTTGGCATGCCGGGGATAGGGGTGGGAAATGAAGGGGCAGGGCAACACGc > 1:42325/1‑151 (MQ=255) gCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCg < 1:676140/151‑1 (MQ=255) cacaGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGGAAAGGGCTGGAAATTGAAGGGGGAGTGCAACACGCCCCG‑CAAccccg > 1:573887/1‑149 (MQ=255) cacaGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCgg < 2:53760/151‑1 (MQ=255) tttCGGTCGCAGTGGCGAGGCGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCCTGCCACCTGGCATGCCGGGAATAGGGCTGGGAAATGAAGGGGGAGTGCAACACGCCCCG‑CCACCCGggcggc > 2:101566/1‑149 (MQ=255) cGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATTTGGCATTCCGGGAATAGGGCTGGAAATTGCAAGGGCAGGGCAACACGCCCCG‑CCACCCGGGCGGCAtt > 2:608760/1‑149 (MQ=255) ggTCGCAGGGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGGTTGCCATCTTGCATGCCGGGAATAAGGGTGGGAATTGGAGGGGGGGTGGAACACGCCCCC‑CCAACCGGGCGGCaattc > 1:26694/1‑146 (MQ=255) gCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGGATAGGGGTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGACGGCATTCCCTAc > 2:788440/1‑150 (MQ=255) gTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGACGGCATTCCATACgttg < 2:573925/151‑1 (MQ=255) tGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGGTTGGCATCTGGCATTCCGGGAATCGAGCCAGAAGTTGAAGGGGCAGTTCCACACCCCCCG‑GCACCCGGGCGGCAGTCCAGACg > 1:800064/1‑147 (MQ=255) gCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGACGGCATTCCATACGTTGTtc < 2:48497/151‑1 (MQ=255) aGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGTGTTGCCATCTGGCATGTCGGGAATAGGGGTGGAAAATGAAGGGGGAGGGAAACACACCCCG‑CAACCCGGACGGGCTTCCATACGTTGTTctct > 1:270670/1‑151 (MQ=255) aGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGGTTGCCATCTTGCATTCCGGGGATAGGGGTGGGAATTGAAGGGGGAGGGCAACACGCCCCGCCAACCCGG‑CGGCATTTCCTACGTTGTtctc > 1:214945/1‑150 (MQ=255) aGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGACGGCATTCCATACGTTGTTctct > 2:742745/1‑151 (MQ=255) cAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCC‑CAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAaccacc < 1:572421/151‑1 (MQ=255) gCGTTGTTGTGCATCAAGCTGAATGCAGGGCGTATGGGCGGGTGTGCCATCTGGCAAGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CACCCCGGACGGCATTCCTTACTTTGTTCTCTTAAGGTTAACCTCCgg < 1:544751/151‑1 (MQ=255) gCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCCTGCCGGGAATAGGGCTGGGAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGACGGCATTCCATACGTTGTTTTCTTAAGGGTAACCACCGGGtt > 1:123690/1‑149 (MQ=255) gCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATtg < 1:142317/150‑1 (MQ=255) tGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGACGGCATTTCATACGTTGTTCTCTTAAGGGTAACCACCGGATtgatg > 2:422510/1‑151 (MQ=255) tGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGGTAAACACCGGATtgat > 2:33146/1‑150 (MQ=255) gTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATtgatg < 2:630390/150‑1 (MQ=255) tcatcaAGCTGAATGCAGGGCGTATTGGCGGGGTTGACATCTGGCATGCCCGGGATAGGGGTGGAAATTGAAGGGGGAGTGCAAAACGCCCCG‑CCAACCGGACGGCCGTCCATACGTTGTTCTCTTAAGGGTAACCACCGGATtgatga > 1:578785/1‑149 (MQ=255) catcaAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCCTGCCGGGAGTAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAgc < 2:665567/150‑1 (MQ=255) catcaAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAgcg < 2:494776/151‑1 (MQ=255) catcaAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAgc > 2:504480/1‑150 (MQ=255) caAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGGTGGGAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGACGGCATTCCATACGTTGTTTTCTTAAGGTTAACCACCGGATTGATGAgcgcgc > 1:153698/1‑151 (MQ=255) caAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAgcgcg < 1:63936/150‑1 (MQ=255) gAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAg < 1:257181/149‑1 (MQ=255) aGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGGTGGAAATTGAAGGGGGCGTGCAACCCGCCCCG‑CAACCCGGACGGCCTTCCCTACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGGGCCCAGTaccccc > 1:301280/1‑150 (MQ=255) aGGGCGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAAAACGCCCCG‑CCACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGGTAACCACCGGATTGATGAGGGCGCACAGTaccacc > 1:243490/1‑150 (MQ=255) cGTATTGGCGGGCTTGCCATCTGGCATTCCGGGAAGATGGGTGGAAAATGAAAGGGCGGGGCAACACGCCCCG‑CAAACCGGACGGCATTCCACACGTTGTTCTCTTAAGGTTAACCACCGGATTTAAGAGCGGTCCCAGTACCACCttttt > 1:240593/1‑151 (MQ=255) cGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCttttt < 2:49329/151‑1 (MQ=255) cGTATTGGCGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCttttt < 2:509780/151‑1 (MQ=255) cGGGCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAg < 2:602116/151‑1 (MQ=255) ggCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCa < 2:854798/151‑1 (MQ=255) ggCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGACGGCATTCCATACGTTGTTCTCTAAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGc > 2:263688/1‑150 (MQ=255) gCTTGCCATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCaa < 1:151920/151‑1 (MQ=255) ccATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTg < 1:856822/151‑1 (MQ=255) cATCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGc < 1:548615/151‑1 (MQ=255) aTCTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCg > 1:349712/1‑151 (MQ=255) cTGGCATGCCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGCCGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGTACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGa > 1:648197/1‑150 (MQ=255) | CTGCTGACGCGAGTTACCGCACATTTCAGCGCTGGCTTGTAAGCCTGCACAGACTTTCGGTCGCAGTGGCGAGGTGAAAATTTTACAGCGTTGCTGTTCATCAAGCTGAATGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCG‑CAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGATGAGCGCGCACAGTACCACCTTTTTTGCACCAGCAAAAGTGCGA > NC_000913/2265086‑2265372 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |