Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,268,435 G→A A203T (GCC→ACC)  yeiR → Zn‑stimulated GTPase involved in zinc homeostasis; mutants are cadmium and EDTA sensitive; Zn(2+) binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,268,4350GA100.0% 177.6 / NA 49A203T (GCC→ACC) yeiRZn‑stimulated GTPase involved in zinc homeostasis; mutants are cadmium and EDTA sensitive; Zn(2+) binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/22);  total (27/22)

CCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCGGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGT  >  NC_000913/2268293‑2268577
                                                                                                                                              |                                                                                                                                              
ccGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGcgcc                                                                                                                                        <  1:143288/151‑1 (MQ=255)
  gTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGcgccgc                                                                                                                                      >  1:782716/1‑151 (MQ=255)
     cgacgCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCgcgc                                                                                                                                    >  2:290936/1‑150 (MQ=255)
     cgacgCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCa                                                                                                                                   <  1:16866/151‑1 (MQ=255)
         gCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATtct                                                                                                                               >  1:688422/1‑151 (MQ=255)
          cccGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATtctc                                                                                                                              <  2:343174/151‑1 (MQ=255)
            cGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCtcat                                                                                                                            >  2:545984/1‑151 (MQ=255)
            cGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCtcat                                                                                                                            >  2:161405/1‑151 (MQ=255)
               aaGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCtcatc                                                                                                                           >  1:570239/1‑149 (MQ=255)
                    aGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGt                                                                                                                    >  2:585979/1‑151 (MQ=255)
                      cAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCg                                                                                                                  >  2:551726/1‑151 (MQ=255)
                           gcTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGaaa                                                                                                             <  2:824775/151‑1 (MQ=255)
                            cTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGaaa                                                                                                             >  1:204944/1‑150 (MQ=255)
                              aCAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGaaaaaa                                                                                                          <  2:710880/151‑1 (MQ=255)
                              aCAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGaaaa                                                                                                            <  2:419075/149‑1 (MQ=255)
                                aGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAgg                                                                                                        <  2:271672/151‑1 (MQ=255)
                                 gCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAggg                                                                                                       >  1:245307/1‑151 (MQ=255)
                                   gTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGtt                                                                                                     <  1:781954/151‑1 (MQ=255)
                                     tggtggCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTa                                                                                                    <  2:837964/150‑1 (MQ=255)
                                       gtggCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTagc                                                                                                  >  2:732466/1‑150 (MQ=255)
                                           cagcaAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGt                                                                                             <  1:665441/151‑1 (MQ=255)
                                           cagcaAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGt                                                                                             >  2:104579/1‑151 (MQ=255)
                                             gcaAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGtt                                                                                            >  1:363803/1‑150 (MQ=255)
                                              caAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTaa                                                                                          <  2:95676/151‑1 (MQ=255)
                                                    ggtggCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTg                                                                                     <  2:117578/150‑1 (MQ=255)
                                                             cGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCAt                                                                            <  2:351395/150‑1 (MQ=255)
                                                               aCAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCa                                                                          >  1:253441/1‑150 (MQ=255)
                                                               aCAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCa                                                                          >  2:227007/1‑150 (MQ=255)
                                                                  attaattCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGc                                                                      <  1:462520/151‑1 (MQ=255)
                                                                     aattCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTgg                                                                   >  2:797587/1‑151 (MQ=255)
                                                                     aattCACAGTGAGCATGGGAAAGTTGACGGTCATATTCTGGATTTACCGCGTCGCAATTTAGCCGAGTTGCCCACCAGTGCCGCGCATTCTCATCAGCACGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAActct                                                                     >  1:41199/1‑149 (MQ=255)
                                                                       ttCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGc                                                                  >  1:684154/1‑150 (MQ=255)
                                                                         cacaGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTc                                                               >  2:807353/1‑151 (MQ=255)
                                                                           caGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGc                                                             >  1:821939/1‑151 (MQ=255)
                                                                                     gggAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAAc                                                    >  2:468712/1‑150 (MQ=255)
                                                                                     gggAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACa                                                   <  2:360656/151‑1 (MQ=255)
                                                                                        aaagcggACAGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCCAGTTACCCACCAGCGCCGGGCACTCTCAGCAGCATGTGGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCg                                                <  1:684909/145‑1 (MQ=255)
                                                                                          aGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCggg                                              <  2:158047/151‑1 (MQ=255)
                                                                                             tGACGGTCATCTTCGGTATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCCTCAGCATGTCGTGAAAACAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCCGGCGTCTCAGCCTGAACAGCGTGct                                            <  1:182309/150‑2 (MQ=255)
                                                                                               aCGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCGGGCAAgg                                         >  1:360144/1‑151 (MQ=255)
                                                                                                       cttctGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCGGGCAAGGATATCAgg                                 >  2:507234/1‑151 (MQ=255)
                                                                                                            ggATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCGGGCAAGGATATCAGGcc                               <  1:439105/148‑1 (MQ=255)
                                                                                                                      cgTCGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCGGGCAAGGATATCAGGCCTGCGGCTGGatat                  <  1:194959/151‑1 (MQ=255)
                                                                                                                         cGCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCGGGCAAGGATATCAGGCCTGCGGCTGGATATTCg               >  2:422940/1‑151 (MQ=255)
                                                                                                                          gCAATTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTTTGAACAGCGGGCAAGGATATCAGGCCTGCGGCTGGATATTCg               >  1:710693/1‑150 (MQ=255)
                                                                                                                            aaTTTAGCCGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCGGGCAAGGATATCAGGCCTGCGGCTGGATATTCGAc             <  2:116144/150‑1 (MQ=255)
                                                                                                                                    cGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCGGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACg    >  1:427938/1‑151 (MQ=255)
                                                                                                                                    cGAGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCGGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACg    <  2:202573/151‑1 (MQ=255)
                                                                                                                                      aGTTGCCCACCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCGGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGt  >  1:811287/1‑151 (MQ=255)
                                                                                                                                              |                                                                                                                                              
CCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAGAGCATCAACGCTGGCGTCGCAGTCTGAACAGCGGGCAAGGATATCAGGCCTGCGGCTGGATATTCGACGCTGATACGGT  >  NC_000913/2268293‑2268577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑