Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,270,278	T→C	R100R (CGT→CGC)	mepS →	murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein, weak murein LD‑carboxypeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,270,278	0	T	C	100.0%	215.3 / NA	59	R100R (CGT→CGC)	mepS	murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein, weak murein LD‑carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/34);  total (25/34)

AGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGTACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTGCGTACGGGTGATTTAGTTCTGTTCCGTGCCGGTTCAACGGGACGCCATGTCGGTATTTAT  >  NC_000913/2270131‑2270425                                                                                                                                                    |                                                                                                                                                    aGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGcaca                                                                                                                                                  <  2:832356/151‑1 (MQ=255)     tctcAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTc                                                                                                                                               <  2:735979/150‑1 (MQ=255)       tcAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGt                                                                                                                                            >  1:67084/1‑151 (MQ=255)        cAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTg                                                                                                                                           <  2:856781/151‑1 (MQ=255)          ggATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTg                                                                                                                                           >  1:214416/1‑149 (MQ=255)          ggATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTg                                                                                                                                           >  2:112931/1‑149 (MQ=255)            aTGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGATCTGGTTTCGTACAGCGCACATTCCGTGAGc                                                                                                                                        >  1:202038/1‑150 (MQ=255)               aaTTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAAtt                                                                                                                                    <  1:654301/151‑1 (MQ=255)               aaTTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAAtt                                                                                                                                    <  1:349213/151‑1 (MQ=255)                    gAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCt                                                                                                                               >  1:39215/1‑151 (MQ=255)                         ccTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGaa                                                                                                                          <  2:129334/151‑1 (MQ=255)                            ggTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAAc                                                                                                                         <  1:488904/149‑1 (MQ=255)                            ggTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACtt                                                                                                                       >  2:832414/1‑151 (MQ=255)                                      gTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGa                                                                                                             <  1:49194/151‑1 (MQ=255)                                         gACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTTCAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACtt                                                                                                          <  1:602110/151‑1 (MQ=255)                                         gACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACtt                                                                                                          >  2:463723/1‑151 (MQ=255)                                           cGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTAc                                                                                                        <  1:854111/151‑1 (MQ=255)                                           cGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTAc                                                                                                        <  2:14538/151‑1 (MQ=255)                                             tAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACg                                                                                                       >  1:235605/1‑150 (MQ=255)                                              aaaaTCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGa                                                                                                      <  1:436235/150‑1 (MQ=255)                                                aaTCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAAca                                                                                                   <  1:697055/151‑1 (MQ=255)                                                aaTCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAAca                                                                                                   <  1:205975/151‑1 (MQ=255)                                                    gcgAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAAcagcag                                                                                               <  1:477868/151‑1 (MQ=255)                                                     cgAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAgg                                                                                              <  2:92689/151‑1 (MQ=255)                                                      gAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAgg                                                                                              >  2:841775/1‑150 (MQ=255)                                                               aTCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTa                                                                                     >  2:632095/1‑150 (MQ=255)                                                                tCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTaa                                                                                    >  2:288313/1‑150 (MQ=255)                                                                   gTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCt                                                                                >  2:584531/1‑151 (MQ=255)                                                                          gACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCg                                                                                                              >  1:430605/1‑114 (MQ=255)                                                                          gACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCg                                                                                                              <  2:430637/114‑1 (MQ=255)                                                                           aCTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCg                                                                        >  2:171025/1‑151 (MQ=255)                                                                               gAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGt                                                                    <  2:221709/151‑1 (MQ=255)                                                                                aaaGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTa                                                                   <  1:555375/151‑1 (MQ=255)                                                                                   ggCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAAtt                                                                <  2:247545/151‑1 (MQ=255)                                                                                    gCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAAttt                                                               <  2:529474/151‑1 (MQ=255)                                                                                     cGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTg                                                              >  1:233417/1‑151 (MQ=255)                                                                                      gTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTGc                                                             >  1:692764/1‑151 (MQ=255)                                                                                        aCGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTGCGt                                                           <  2:359747/151‑1 (MQ=255)                                                                                         cGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTGCg                                                            <  1:465046/149‑1 (MQ=255)                                                                                          gTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTGCGTa                                                          >  1:162768/1‑150 (MQ=255)                                                                                          gTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTGCGTAc                                                         <  2:803040/151‑1 (MQ=255)                                                                                            tATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTGCGTACgg                                                       >  1:677789/1‑151 (MQ=255)                                                                                             aTCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTGCGTACggg                                                      <  1:546256/151‑1 (MQ=255)                                                                                                  cTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTGGGTACGGGTGAtt                                                 >  1:191662/1‑151 (MQ=255)                                                                                                     ggcggcAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTGCGTACGGGTGATTTAg                                              <  1:526520/151‑1 (MQ=255)                                                                                                       cggcAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTGCGTACGGGTGATTTAGtt                                            <  1:804642/151‑1 (MQ=255)                                                                                                          cagcaCTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTGCGTACGGGTGATTTAGTTCTg                                         >  2:440957/1‑151 (MQ=255)                                                                                                                  aaaaaGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTGCGTACGGGTGATTTAGTTCTGTTCCGTGc                                 >  2:114896/1‑151 (MQ=255)                                                                                                                  aaaaaGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTGCGTACGGGTGATTTAGTTCTGTTCCGTGc                                 >  1:342116/1‑151 (MQ=255)                                                                                                                     aaGGTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTGCGTACGGGTGATTTAGTTCTGTTCCGTGCCgg                              <  1:115398/151‑1 (MQ=255)                                                                                                                       ggTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTGCGTACGGGTGATTTAGTTCTGTTCCGTGCCGGtt                            <  1:727911/151‑1 (MQ=255)                                                                                                                       ggTATCGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTGCGTACGGGTGATTTAGTTCTGTTCCGTGCCGGt                             >  2:525112/1‑150 (MQ=255)                                                                                                                          aTCGGTTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTGTCCCGCAGTAATTTGCGTACGGGTGATTTAGTTCTGTTCCGTGCCGGTTCa                          <  2:469233/150‑1 (MQ=255)                                                                                                                            cGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTGCGTACGGGTGATTTAGTTCTGTTCCGTGCCGGTTCAACg                       >  1:221863/1‑151 (MQ=255)                                                                                                                            cGATTGTTCTGGTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTGCGTACGGGTGATTTAGTTCTGTTCCGTGCCGGTTCAACg                       >  1:197820/1‑151 (MQ=255)                                                                                                                                       gTTTCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTGCGTACGGGTGATTTAGTTCTGTTCCGTGCCGGTTCAACGGGACGCCATGt            <  2:509651/151‑1 (MQ=255)                                                                                                                                         ttCGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTGCGTACGGGTGATTTAGTTCTGTTCCGTGCCGGTTCAACGGGACGCCATGTc           <  2:777443/150‑1 (MQ=255)                                                                                                                                           cGTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTGCGTACGGGTGATTTAGTTCTGTTCCGTGCCGGTTCAACGGGACGCCATGTCGGt        <  2:803564/151‑1 (MQ=255)                                                                                                                                            gTACAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAGCAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAAGTTGCGTACGGGTGATTTAGTTCTGTTCCGTGCCGGTTCAACGGGACGCCATGTCgg         <  2:118867/149‑1 (MQ=255)                                                                                                                                              aCAGCGCACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTGCGTACGGGTGATTTAGTTCTGTTCCGTGCCGGTTCAACGGGACGCCATGTCGGTAtt     <  1:30330/151‑1 (MQ=255)                                                                                                                                                 gcgcACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTGCGTACGGGTGATTTAGTTCTGTTCCGTGCCGGTTCAACGGGACGCCATGTCGGTATTtat  <  1:296072/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    AGCTTCTCAGGATGAATTTGAAAACCTGGTTCGTAATGTCGACGTAAAATCGCGAATTATGGATCAGTATGCTGACTGGAAAGGCGTACGTTATCGTCTGGGCGGCAGCACTAAAAAAGGTATCGATTGTTCTGGTTTCGTACAGCGTACATTCCGTGAGCAATTTGGCTTAGAACTTCCGCGTTCGACTTACGAACAGCAGGAAATGGGTAAATCTGTTTCCCGCAGTAATTTGCGTACGGGTGATTTAGTTCTGTTCCGTGCCGGTTCAACGGGACGCCATGTCGGTATTTAT  >  NC_000913/2270131‑2270425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑