Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,276,922	T→C	M208T (ATG→ACG)	yejF →	microcin C ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,276,922	0	T	C	100.0%	184.6 / NA	51	M208T (ATG→ACG)	yejF	microcin C ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/32);  total (19/32)

GGCGGCGAACGGCAGCGGGTGATGATTGCGATGGCGCTGTTAACGCGACCGGAATTATTAATTGCCGATGAACCGACCACCGCACTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATATGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCCGCTACGCTATTTGCATCACCCACTCATCCTTACACACAAAAGC  >  NC_000913/2276774‑2277065                                                                                                                                                     |                                                                                                                                                ggcggcGAACGGCAGCGGGTGATGATTGCGATGGCGCTGTTAACGCGACCGGAATTATTAATTGCCGATGAACCGACCACCGCACTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACgg                                                                                                                                               >  1:143630/1‑151 (MQ=255)  gcggcgAACGGCAGCGGGTGATGATTGCGATGGCGCTGTTAACGCGACCGGAATTATTAATTGCCGATGAACCGACCACCGCACTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACggg                                                                                                                                              >  2:122559/1‑151 (MQ=255)    ggcgAACGGCAGCGGGTGATGATTGCGATGGCGCTGTTAACGCGACCGGAATTATTAATTGCCGATGAACCGACCACCGCACTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGc                                                                                                                                             >  1:610645/1‑150 (MQ=255)          cGGCAGCGGGTGATGATTGCGATGGCGCTGTTAACGCGACCGGAATTATTAATTGCCGATGAACCGACCACCGCACTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCt                                                                                                                                        <  1:511132/149‑1 (MQ=255)             cAGCGGGTGATGATTGCGATGGCGCTGTTAACGCGACCGGAATTATTAATTGCCGATGAACCGACCACCGCACTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTtta                                                                                                                                   <  2:104512/151‑1 (MQ=255)                      atgatTGCGATGGCGCTGTTAACGCGACCGGAATTATTAATTGCCGATGAACCGACCACCGCACTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATa                                                                                                                          <  2:268541/151‑1 (MQ=255)                        gatTGCGATGGCGCTGTTAACGCGACCGGAATTATTAATTGCCGATGAACCGACCACCGCACTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATaa                                                                                                                         <  2:141656/150‑1 (MQ=255)                           tGCGATGGCGCTGTTAACGCGACCGGAATTATTAATTGCCGATGAACCGACCACCGCACTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTc                                                                                                                     >  2:400674/1‑151 (MQ=255)                            gCGATGGCGCTGTTAACGCGACCGGAATTATTAATTGCCGATGAACCGACCACCGCACTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCa                                                                                                                    <  2:419415/151‑1 (MQ=255)                            gCGACGGCGCTGTTAGCGAGTGCGGAGTTATTAGTTTCCGATGTACCGCCCACAGCGCTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTATTGCGCGAAGTGCAAGGCGAGCTGGATGCGGGCATGCTGTTTTTTAATCATaa                                                                                                                         <  1:511043/146‑1 (MQ=255)                                 ggCGCTGTTAACGCGACCGGAATTATTAATTGCCGATGAACCGACCACCGCACTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCAtt                                                                                                               <  2:489862/151‑1 (MQ=255)                                          aaCGCGACCGGAATTATTAATTGCCGATGAACCGACCACCGCACTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGaaa                                                                                                       <  2:587985/150‑1 (MQ=255)                                           aCGCGACCGGAATTATTAATTGCCGATGAACCGACCACCGCACTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAAc                                                                                                     <  2:58385/151‑1 (MQ=255)                                            cgcgACCGGAATTATTAATTGCCGATGAACCGACCACCGCACTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACt                                                                                                    >  1:738726/1‑151 (MQ=255)                                                   ggAATTATTAATTGCCGATGAACCGACCACCGCACTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCAc                                                                                             >  1:246717/1‑151 (MQ=255)                                                   ggAATTATTAATTGCCGATGAACCGACCACCGCACTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCAc                                                                                             >  1:310022/1‑151 (MQ=255)                                                   ggAATTATTAATTGCCGATGAACCGACCACCGCACTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCAc                                                                                             >  1:71703/1‑151 (MQ=255)                                                                    aTGAACCGACCACCGCACTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGc                                                                             >  2:69071/1‑150 (MQ=255)                                                                      gAACCGACCACCGCACTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCaa                                                                           <  1:222182/150‑1 (MQ=255)                                                                       aaCCGACCACCGCACTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCaaaa                                                                         <  1:546601/151‑1 (MQ=255)                                                                         ccGACCACCGCACTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCGCCGCGTGGCGGTAATGCAAAACg                                                                       <  1:511947/151‑1 (MQ=255)                                                                         ccGACCACCGCACTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACg                                                                       <  2:758971/151‑1 (MQ=255)                                                                          cGACCACCGCACTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACgg                                                                      <  1:303049/151‑1 (MQ=255)                                                                            accaccGCACTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTc                                                                    <  1:314191/151‑1 (MQ=255)                                                                               accGCACTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGTAAAACGGTCGCt                                                                 <  1:533951/151‑1 (MQ=255)                                                                                     cTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCg                                                           >  2:75723/1‑151 (MQ=255)                                                                                       ggACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCg                                                           >  1:263954/1‑149 (MQ=255)                                                                                               ctGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCGAGCaaaa                                                    <  2:461750/148‑1 (MQ=255)                                                                                               ctGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCGAGCAAAAt                                                   <  1:711770/149‑1 (MQ=255)                                                                                                          cAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCcgctacgc                                      >  2:756820/1‑151 (MQ=255)                                                                                                            gATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCcgctacgct                                     >  2:121607/1‑150 (MQ=255)                                                                                                            gATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCcgctacgct                                     >  1:579434/1‑150 (MQ=255)                                                                                                             aTTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCCGCTACGCTAt                                   <  2:24661/151‑1 (MQ=255)                                                                                                                 tACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCCGCTACGCTATTTg                                <  1:683480/150‑1 (MQ=255)                                                                                                                   cAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCCGCTACGCTATTTGCa                              <  1:459034/150‑1 (MQ=255)                                                                                                                     gCTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCCGCTACGCTATTTGCATc                            >  2:733230/1‑150 (MQ=255)                                                                                                                      cTGTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCCGCTACGCTATTTGCATCAc                          >  2:433200/1‑151 (MQ=255)                                                                                                                        gTTGCGCGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCCGCTACGCTATTTGCATCAcc                         <  2:467017/150‑1 (MQ=255)                                                                                                                           gcgcGAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCCGCTACGCTATTTGCATCACCCAc                      <  2:196030/150‑1 (MQ=255)                                                                                                                            cgcgAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCCGCTACGCTATTTGCATCACCCACTc                    <  2:774657/151‑1 (MQ=255)                                                                                                                              cgAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAATCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCCGCTACGCTATTTGCATCACCCACTCAt                  <  2:569877/151‑1 (MQ=255)                                                                                                                              cgAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCCGCTACGCTATTTGCATCACCCACTCAt                  <  1:338629/151‑1 (MQ=255)                                                                                                                              cgAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCCGCTACGCTATTTGCATCACCCACTCAt                  <  1:191312/151‑1 (MQ=255)                                                                                                                               gAACTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCCGCTACGCTATTTGCATCACCCACTCATc                 <  1:114179/151‑1 (MQ=255)                                                                                                                                aaCTGCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCCGCTACGCTATTTGCATCACCCACTCATcc                <  1:335494/151‑1 (MQ=255)                                                                                                                                    gCAAGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCCGCTACGCTATTTGCATCACCCACTCATCCtt              <  2:51406/149‑1 (MQ=255)                                                                                                                                      aaGGCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCCGCTACGCTATTTGCATCACCCACTCATCCTTaca           >  2:614365/1‑150 (MQ=255)                                                                                                                                        ggCGAGCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCCGCTACGCTATTTGCATCACCCACTCATCCTTacacac        <  2:112841/151‑1 (MQ=255)                                                                                                                                             gCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCCGCTACGCTATTTGCATCACCCACTCATCCTTACACACaaa     >  2:121219/1‑149 (MQ=255)                                                                                                                                             gCTGAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCCGCTACGCTATTTGCATCACCCACTCATCCTTACACACaaa     >  1:750793/1‑149 (MQ=255)                                                                                                                                                gAATACGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCCGCTACGCTATTTGCATCACCCACTCATCCTTACACACAAAAGc  <  2:531577/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                GGCGGCGAACGGCAGCGGGTGATGATTGCGATGGCGCTGTTAACGCGACCGGAATTATTAATTGCCGATGAACCGACCACCGCACTGGACGTCTCTGTCCAGGCGCAGATTTTACAGCTGTTGCGCGAACTGCAAGGCGAGCTGAATATGGGCATGCTGTTTATTACTCATAACCTCAGCATTGTCAGAAAACTGGCCCACCGCGTGGCGGTAATGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCCGCTACGCTATTTGCATCACCCACTCATCCTTACACACAAAAGC  >  NC_000913/2276774‑2277065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑