Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,281,016 C→T Q129* (CAG→TAG)  yejH → putative ATP‑dependent DNA or RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,281,0160CT100.0% 186.2 / NA 50Q129* (CAG→TAG) yejHputative ATP‑dependent DNA or RNA helicase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/22);  total (28/22)

GCTAAAGCGCAAAGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTT  >  NC_000913/2280877‑2281163
                                                                                                                                           |                                                                                                                                                   
gCTAAAGCGCAAAGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCaa                                                                                                                                          <  2:452023/151‑1 (MQ=255)
  tAAAGCGCAAAGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAAt                                                                                                                                        >  2:248755/1‑151 (MQ=255)
   aaaGCGCAAAGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAAt                                                                                                                                        <  2:136493/150‑1 (MQ=255)
     aGCGCAAAGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCct                                                                                                                                     >  2:855297/1‑151 (MQ=255)
      gcgcAAAGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCctc                                                                                                                                    >  2:331290/1‑151 (MQ=255)
        gcAAAGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCctcac                                                                                                                                  <  2:54040/151‑1 (MQ=255)
         cAAAGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCctcact                                                                                                                                 >  1:112418/1‑151 (MQ=255)
                  ccACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACa                                                                                                                        <  1:689157/151‑1 (MQ=255)
                          aaGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGaa                                                                                                                <  1:715643/151‑1 (MQ=255)
                            gTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATc                                                                                                              <  2:707599/151‑1 (MQ=255)
                            gTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATc                                                                                                              <  1:625361/151‑1 (MQ=255)
                              ggTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAATAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATccc                                                                                                            <  1:699037/151‑1 (MQ=255)
                                 aTTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCa                                                                                                          <  2:417839/150‑1 (MQ=255)
                                 aTTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCAc                                                                                                         >  1:56971/1‑151 (MQ=255)
                                        aGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGc                                                                                                   <  2:710507/150‑1 (MQ=255)
                                            tGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCctgct                                                                                              >  1:88203/1‑151 (MQ=255)
                                              cAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTgg                                                                                            <  2:393108/151‑1 (MQ=255)
                                                      cGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACt                                                                                    <  1:631226/151‑1 (MQ=255)
                                                          cGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGcc                                                                                 >  1:474611/1‑150 (MQ=255)
                                                          cGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGcc                                                                                 >  2:704399/1‑150 (MQ=255)
                                                             aaTCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGc                                                                             >  2:241963/1‑151 (MQ=255)
                                                                  tGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCtttt                                                                        >  1:557576/1‑151 (MQ=255)
                                                                  tGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCtt                                                                          <  1:565020/149‑1 (MQ=255)
                                                                   gATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATccc                                                                                                            >  2:594157/1‑114 (MQ=255)
                                                                   gATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATccc                                                                                                            <  1:594119/114‑1 (MQ=255)
                                                                    aTGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCg                                                                      >  2:253911/1‑151 (MQ=255)
                                                                    aTGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCg                                                                      >  1:738159/1‑151 (MQ=255)
                                                                          ttCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTggg                                                                >  2:543136/1‑151 (MQ=255)
                                                                          ttCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTggg                                                                >  1:189890/1‑151 (MQ=255)
                                                                            cAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGc                                                               >  2:335719/1‑150 (MQ=255)
                                                                                gTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAgg                                                          <  1:294290/151‑1 (MQ=255)
                                                                                   aaTTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTg                                                       >  1:762100/1‑151 (MQ=255)
                                                                                        tCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATc                                                   <  1:331267/150‑1 (MQ=255)
                                                                                         cGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCt                                                  >  1:317732/1‑150 (MQ=255)
                                                                                             gTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCa                                              <  1:559200/150‑1 (MQ=255)
                                                                                               tGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGt                                            <  2:403418/150‑1 (MQ=255)
                                                                                                   tGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCAt                                       >  1:749514/1‑151 (MQ=255)
                                                                                                       gATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATc                                   >  1:513583/1‑151 (MQ=255)
                                                                                                              gTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCAt                            >  2:95626/1‑151 (MQ=255)
                                                                                                                 aCCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGt                         >  2:544655/1‑151 (MQ=255)
                                                                                                                 aCCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGt                         >  1:501274/1‑151 (MQ=255)
                                                                                                                  ccGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTa                        <  2:611605/151‑1 (MQ=255)
                                                                                                                        tGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACgcggc                  >  1:150835/1‑151 (MQ=255)
                                                                                                                          gTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGa                <  2:432785/151‑1 (MQ=255)
                                                                                                                             aCGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATga             <  2:176801/151‑1 (MQ=255)
                                                                                                                                  gAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGaaa         <  1:403764/150‑1 (MQ=255)
                                                                                                                                   aaGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGc       >  2:173951/1‑151 (MQ=255)
                                                                                                                                     gagagCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGccc     >  2:231942/1‑151 (MQ=255)
                                                                                                                                      agagCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCt    >  1:777973/1‑151 (MQ=255)
                                                                                                                                        agCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCttt  >  2:695173/1‑151 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
GCTAAAGCGCAAAGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTT  >  NC_000913/2280877‑2281163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: