Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,281,016	C→T	Q129* (CAG→TAG)	yejH →	putative ATP‑dependent DNA or RNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,281,016	0	C	T	100.0%	186.2 / NA	50	Q129* (CAG→TAG)	yejH	putative ATP‑dependent DNA or RNA helicase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/22);  total (28/22)

GCTAAAGCGCAAAGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTT  >  NC_000913/2280877‑2281163                                                                                                                                            |                                                                                                                                                    gCTAAAGCGCAAAGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCaa                                                                                                                                          <  2:452023/151‑1 (MQ=255)   tAAAGCGCAAAGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAAt                                                                                                                                        >  2:248755/1‑151 (MQ=255)    aaaGCGCAAAGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAAt                                                                                                                                        <  2:136493/150‑1 (MQ=255)      aGCGCAAAGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCct                                                                                                                                     >  2:855297/1‑151 (MQ=255)       gcgcAAAGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCctc                                                                                                                                    >  2:331290/1‑151 (MQ=255)         gcAAAGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCctcac                                                                                                                                  <  2:54040/151‑1 (MQ=255)          cAAAGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCctcact                                                                                                                                 >  1:112418/1‑151 (MQ=255)                   ccACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACa                                                                                                                        <  1:689157/151‑1 (MQ=255)                           aaGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGaa                                                                                                                <  1:715643/151‑1 (MQ=255)                             gTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATc                                                                                                              <  2:707599/151‑1 (MQ=255)                             gTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATc                                                                                                              <  1:625361/151‑1 (MQ=255)                               ggTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAATAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATccc                                                                                                            <  1:699037/151‑1 (MQ=255)                                  aTTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCa                                                                                                          <  2:417839/150‑1 (MQ=255)                                  aTTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCAc                                                                                                         >  1:56971/1‑151 (MQ=255)                                         aGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGc                                                                                                   <  2:710507/150‑1 (MQ=255)                                             tGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCctgct                                                                                              >  1:88203/1‑151 (MQ=255)                                               cAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTgg                                                                                            <  2:393108/151‑1 (MQ=255)                                                       cGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACt                                                                                    <  1:631226/151‑1 (MQ=255)                                                           cGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGcc                                                                                 >  1:474611/1‑150 (MQ=255)                                                           cGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGcc                                                                                 >  2:704399/1‑150 (MQ=255)                                                              aaTCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGc                                                                             >  2:241963/1‑151 (MQ=255)                                                                   tGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCtttt                                                                        >  1:557576/1‑151 (MQ=255)                                                                   tGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCtt                                                                          <  1:565020/149‑1 (MQ=255)                                                                    gATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATccc                                                                                                            >  2:594157/1‑114 (MQ=255)                                                                    gATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATccc                                                                                                            <  1:594119/114‑1 (MQ=255)                                                                     aTGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCg                                                                      >  2:253911/1‑151 (MQ=255)                                                                     aTGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCg                                                                      >  1:738159/1‑151 (MQ=255)                                                                           ttCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTggg                                                                >  2:543136/1‑151 (MQ=255)                                                                           ttCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTggg                                                                >  1:189890/1‑151 (MQ=255)                                                                             cAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGc                                                               >  2:335719/1‑150 (MQ=255)                                                                                 gTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAgg                                                          <  1:294290/151‑1 (MQ=255)                                                                                    aaTTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTg                                                       >  1:762100/1‑151 (MQ=255)                                                                                         tCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATc                                                   <  1:331267/150‑1 (MQ=255)                                                                                          cGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCt                                                  >  1:317732/1‑150 (MQ=255)                                                                                              gTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCa                                              <  1:559200/150‑1 (MQ=255)                                                                                                tGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGt                                            <  2:403418/150‑1 (MQ=255)                                                                                                    tGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCAt                                       >  1:749514/1‑151 (MQ=255)                                                                                                        gATGAATGTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATc                                   >  1:513583/1‑151 (MQ=255)                                                                                                               gTCACCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCAt                            >  2:95626/1‑151 (MQ=255)                                                                                                                  aCCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGt                         >  2:544655/1‑151 (MQ=255)                                                                                                                  aCCGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGt                         >  1:501274/1‑151 (MQ=255)                                                                                                                   ccGTATTGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTa                        <  2:611605/151‑1 (MQ=255)                                                                                                                         tGGTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACgcggc                  >  1:150835/1‑151 (MQ=255)                                                                                                                           gTGACGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGa                <  2:432785/151‑1 (MQ=255)                                                                                                                              aCGATGAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATga             <  2:176801/151‑1 (MQ=255)                                                                                                                                   gAAGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGaaa         <  1:403764/150‑1 (MQ=255)                                                                                                                                    aaGAGAGCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGc       >  2:173951/1‑151 (MQ=255)                                                                                                                                      gagagCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGccc     >  2:231942/1‑151 (MQ=255)                                                                                                                                       agagCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCt    >  1:777973/1‑151 (MQ=255)                                                                                                                                         agCTAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCttt  >  2:695173/1‑151 (MQ=255)                                                                                                                                            |                                                                                                                                                    GCTAAAGCGCAAAGAGAGCCACGGTAAAGTGGTATTTGGCAGCGTGCAGTCTGTCGCCCGTAATCTTGATGCCTTTCAGGGTGAATTTTCGCTGTTGATTGTCGATGAATGTCACCGTATTGGTGACGATGAAGAGAGCCAGTATCAGCAAATCCTCACTCACCTGACAAAAGTGAATCCCCACTTACGCCTGCTGGGGCTGACTGCCACGCCTTTTCGATTGGGCAAAGGCTGGATCTACCAGTTTCATTATCACGGCATGGTACGCGGCGATGAGAAAGCCCTTT  >  NC_000913/2280877‑2281163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑