Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	327,536	A→G	S400P (TCG→CCG)	betB ←	betaine aldehyde dehydrogenase, NAD‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	327,536	0	A	G	100.0%	174.2 / NA	52	S400P (TCG→CCG)	betB	betaine aldehyde dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/22);  total (30/22)

GGGATTCGCCCCAGGTGTTGATCCAGCAAATACCCGCTTCCAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAACCCATGCGCCGTTATCGAAGCCATCGCCTTTCAGTACATCGCCGCCGCACAGT  >  NC_000913/327392‑327681                                                                                                                                                 |                                                                                                                                                  gggATTCGCCCCAGGTGTTGATCCAGCAAATACCCGCTTCCAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCG‑GGCGAATGACTTCGTCTTCCGGCTCGTAg                                                                                                                                            <  2:836444/151‑1 (MQ=255)    aTTCGCCCCAGGTGTTGATCCAGCAAATACCCGCTTCCAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGt                                                                                                                                          >  2:705343/1‑151 (MQ=255)           ccAGGTGTTGATCCAGCAAATACCCGCTTCCAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATg                                                                                                                                   >  1:845145/1‑151 (MQ=255)           ccAGGTGTTGATCCAGCAAATACCCGCTTCCAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATg                                                                                                                                   >  1:598190/1‑151 (MQ=255)                 gtTGATCCAGCAAATACCCGCTTCCAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACAt                                                                                                                              <  2:132221/150‑1 (MQ=255)                   tGATCCAGCAAATACCCGCTTCCAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCAc                                                                                                                           >  2:612737/1‑151 (MQ=255)                         aGCAAATACCCGCTTCCAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGccc                                                                                                                     >  2:473607/1‑151 (MQ=255)                          gCAAATACCCGCTTCCAGCTGATGAATGACGCGATGCGCGCGGGTCAGGTCCGCTGTCACGATGCCCGACGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCg                                                                                                                    >  1:301406/1‑151 (MQ=255)                            aaaTACCCGCTTCCAGCTGATGAATGACGCGATGCGCGCGGTTAAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTAGTATTCCGGCTTGTAGGTCAGAATGGACATCACt                                                                                                                          >  1:375067/1‑143 (MQ=255)                             aaTACCCGCTTCCAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGa                                                                                                                   >  2:50645/1‑149 (MQ=255)                              aTACCCGCTTCTAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGa                                                                                                                <  1:369115/151‑1 (MQ=255)                              aTACCCGCTTCCAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGa                                                                                                                <  1:304090/151‑1 (MQ=255)                               tACCCGCTTCCAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGa                                                                                                                   >  1:491726/1‑147 (MQ=255)                                aCCCGCTTCCAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGa                                                                                                                   >  1:671356/1‑146 (MQ=255)                                aCCCGCTTCCAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGAt                                                                                                               <  2:613109/150‑1 (MQ=255)                                    gCTTCCAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCtt                                                                                                          >  2:434518/1‑151 (MQ=255)                                       tCCAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTc                                                                                                         <  1:473571/149‑1 (MQ=255)                                            cTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTcacgcacg                                                                                                  >  2:335346/1‑151 (MQ=255)                                              gatgaATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTcacgcacg                                                                                                  >  1:661485/1‑149 (MQ=255)                                               atgaatgaCGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATg                                                                                               >  1:606498/1‑151 (MQ=255)                                                   atgaCGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGt                                                                                             >  2:148884/1‑149 (MQ=255)                                                   atgaCGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCa                                                                                           <  1:153727/151‑1 (MQ=255)                                                    tgaCGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGACCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCAAAGATCTCTTCACGCACGATGCTCat                                                                                          >  1:553680/1‑151 (MQ=255)                                                           aTGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATAtcgtc                                                                                    >  1:677019/1‑150 (MQ=255)                                                                     ttCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCgg                                                                         >  1:44667/1‑151 (MQ=255)                                                                        aGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTg                                                                       >  2:317748/1‑150 (MQ=255)                                                                              gCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTg                                                                >  2:325432/1‑151 (MQ=255)                                                                                 gTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTc                                                              <  2:517231/150‑1 (MQ=255)                                                                                 gTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCg                                                             <  2:765709/151‑1 (MQ=255)                                                                                    aCGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTg                                                          <  2:434460/151‑1 (MQ=255)                                                                                        tGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAAc                                                      >  2:70248/1‑151 (MQ=255)                                                                                          cccGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAAccc                                                    >  2:304187/1‑151 (MQ=255)                                                                                            cgccgcCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAACCCAt                                                  <  2:44689/151‑1 (MQ=255)                                                                                             gccgccAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAACCCATg                                                 >  2:595724/1‑151 (MQ=255)                                                                                              ccgccAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAACCCATgc                                                <  2:550172/151‑1 (MQ=255)                                                                                               cgccAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAACCCATgc                                                >  1:428018/1‑150 (MQ=255)                                                                                                gccAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAACCCATgcgc                                              <  2:627931/151‑1 (MQ=255)                                                                                                   aGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAACCCATGCGCCGt                                           <  1:805949/151‑1 (MQ=255)                                                                                                      ccGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAACCCATGCGCCGTTAt                                        <  2:811755/151‑1 (MQ=255)                                                                                                      ccGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCATCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAACCCATGCGCCGTTAt                                        >  1:449371/1‑151 (MQ=255)                                                                                                        gTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAACCCATGCGCCGTTATCg                                      >  1:275032/1‑151 (MQ=255)                                                                                                          aGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAACCCATGCGCCGTTATCGaa                                    <  1:749617/151‑1 (MQ=255)                                                                                                            tCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAACCCATGCGCCGTTATCGAAGc                                  >  2:806966/1‑151 (MQ=255)                                                                                                             cGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAACCCATGCGCCGTTATCGAAGcc                                 <  1:620873/151‑1 (MQ=255)                                                                                                                 aTCGTTAGCGCGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAACCCATGCGCCGTTATCGAAGCCAttg                             <  2:158568/151‑3 (MQ=255)                                                                                                                        gcgcGGCGAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAACCCATGCGCCGTTATCGAAGCCATCGCCTTTc                       >  2:737916/1‑150 (MQ=255)                                                                                                                           cggcgAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAACCCATGCGCCGTTATCGAAGCCATCGCCTTTCAGt                    >  2:24558/1‑150 (MQ=255)                                                                                                                             gcgAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAACCCATGCGCCGTTATCGAAGCCATCGCCTTTCAGTACa                 <  2:724450/151‑1 (MQ=255)                                                                                                                              cgAATGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAACCCATGCGCCGTTATCGAAGCCATCGCCTTTCAGTACAt                <  2:729547/151‑1 (MQ=255)                                                                                                                                aaTGACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAACCCATGCGCCGTTATCGAAGCCATCGCCTTTCAGTACATcg              <  1:549713/151‑1 (MQ=255)                                                                                                                                   gACTTCGTCTTCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAACCCATGCGCCGTTATCGAAGCCATCGCCTTTCAGTACATcgcc            <  1:628630/150‑1 (MQ=255)                                                                                                                                             tCCGGCTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAACCCATGCGCCGTTATCGAAGCCATCGCCTTTCAGTACATCGCCGCCGCACAGt  >  1:626605/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                                  GGGATTCGCCCCAGGTGTTGATCCAGCAAATACCCGCTTCCAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAACCCATGCGCCGTTATCGAAGCCATCGCCTTTCAGTACATCGCCGCCGCACAGT  >  NC_000913/327392‑327681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑