Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,398,634	T→C	Q511R (CAG→CGG)	nuoG ←	NADH:ubiquinone oxidoreductase, chain G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,398,634	0	T	C	100.0%	190.1 / NA	54	Q511R (CAG→CGG)	nuoG	NADH:ubiquinone oxidoreductase, chain G
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/30);  total (24/30)

GCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATG  >  NC_000913/2398486‑2398779                                                                                                                                                     |                                                                                                                                                  gcgTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGa                                                                                                                                                 <  2:231235/151‑1 (MQ=255)  cgTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGa                                                                                                                                                 >  2:420546/1‑150 (MQ=255)     ccGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCTCGACGTTAGCCGCCGCCCGAATc                                                                                                                                              <  1:360352/150‑1 (MQ=255)       ggTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCAcc                                                                                                                                           >  1:251111/1‑151 (MQ=255)        gTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACct                                                                                                                                          <  2:24210/151‑1 (MQ=255)        gTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACct                                                                                                                                          <  1:147874/151‑1 (MQ=255)             cAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTa                                                                                                                                       >  1:776878/1‑149 (MQ=255)               gTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCt                                                                                                                                   <  2:504834/151‑1 (MQ=255)                 tCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTa                                                                                                                                  <  2:809686/150‑1 (MQ=255)                    gTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCgg                                                                                                                              >  2:136295/1‑151 (MQ=255)                       aaCGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGt                                                                                                                           <  2:243440/151‑1 (MQ=255)                       aaCGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGt                                                                                                                           <  1:619916/151‑1 (MQ=255)                       aaCGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGt                                                                                                                           >  1:186112/1‑151 (MQ=255)                            ttcttcAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTc                                                                                                                      >  1:699901/1‑151 (MQ=255)                            ttcttcAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTc                                                                                                                      >  1:308295/1‑151 (MQ=255)                            ttcttcAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTc                                                                                                                      >  2:481838/1‑151 (MQ=255)                                tcAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTccc                                                                                                                    <  2:202602/149‑1 (MQ=255)                                 cAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGga                                                                                                                 <  1:613838/151‑1 (MQ=255)                                  aaGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGgag                                                                                                                <  2:50584/151‑1 (MQ=255)                                    gCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCg                                                                                                                   >  2:517806/1‑146 (MQ=255)                                    gCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGAt                                                                                                              <  1:391550/151‑1 (MQ=255)                                        aCCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATc                                                                                                          <  2:285809/151‑1 (MQ=255)                                        aCCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATc                                                                                                          <  1:188732/151‑1 (MQ=255)                                          cGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCaa                                                                                                        <  2:334126/151‑1 (MQ=255)                                           gCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAAc                                                                                                       <  2:293687/151‑1 (MQ=255)                                            ccACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACg                                                                                                      >  2:390335/1‑151 (MQ=255)                                             cACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACgg                                                                                                     >  2:184010/1‑151 (MQ=255)                                                      tGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGc                                                                                            >  1:418764/1‑151 (MQ=255)                                                      tGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGc                                                                                            >  2:536338/1‑151 (MQ=255)                                                       gCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCa                                                                                           <  2:500155/151‑1 (MQ=255)                                                         ccAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCAcc                                                                                         >  2:654581/1‑151 (MQ=255)                                                          cAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCt                                                                                        <  1:653217/151‑1 (MQ=255)                                                           aGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCt                                                                                        >  1:129/1‑150 (MQ=255)                                                                      gTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGc                                                                            >  2:647003/1‑151 (MQ=255)                                                                        tGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCAc                                                                          >  2:53641/1‑151 (MQ=255)                                                                                cGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGt                                                                  <  1:189602/151‑1 (MQ=255)                                                                                        cATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGAtttt                                                           >  2:435204/1‑150 (MQ=255)                                                                                        cATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGAtttt                                                           <  2:140067/150‑1 (MQ=255)                                                                                            gTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATtttgcttt                                                      >  2:481089/1‑151 (MQ=255)                                                                                                  ccGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCt                                                <  2:856443/151‑1 (MQ=255)                                                                                                    gACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCa                                              <  2:356552/151‑1 (MQ=255)                                                                                                           gcgcCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGa                                       <  2:418797/151‑1 (MQ=255)                                                                                                              ccgcGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATAc                                    <  2:269233/151‑1 (MQ=255)                                                                                                                 cgACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGt                                 <  2:343675/151‑1 (MQ=255)                                                                                                                   aCCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCg                                  <  1:807637/148‑1 (MQ=255)                                                                                                                       ttCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAAcc                            <  2:166674/150‑1 (MQ=255)                                                                                                                       ttCAGGGCTTTCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCg                           >  1:468505/1‑151 (MQ=255)                                                                                                                                ttCGCGACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCa                   <  2:732768/150‑1 (MQ=255)                                                                                                                                  cgcgACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCag                  >  2:734469/1‑149 (MQ=255)                                                                                                                                   gcgACGTTAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGt               >  1:183305/1‑151 (MQ=255)                                                                                                                                          tAGCCGCCGCCCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATcc          >  2:703004/1‑149 (MQ=255)                                                                                                                                              cgccgccCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAgcgc     >  1:126812/1‑150 (MQ=255)                                                                                                                                              cgccgccCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCa    <  1:217330/151‑1 (MQ=255)                                                                                                                                                ccgccCGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATg  <  1:702960/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  GCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGACGGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATG  >  NC_000913/2398486‑2398779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑