Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,420,100	G→A	A129V (GCG→GTG)	yfcF ←	glutathione S‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,420,100	0	G	A	100.0%	175.4 / NA	51	A129V (GCG→GTG)	yfcF	glutathione S‑transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/24);  total (27/24)

GCGGTTAATCATTAGCGCCAGATCAGTATCAGCAATGCACCATTCACCAAATAAATTCGGCTGACCCAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTT  >  NC_000913/2419955‑2420247                                                                                                                                                  |                                                                                                                                                    gCGGTTAATCCTTAGCGCCAGAACAGTAACAGCAATGCACCAATCCCCAAATAAATTCGGCTGGCCCAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCAccccc                                                                                                                                                <  2:174443/151‑1 (MQ=255)          cATTAGCGCCAGATCAGTATCAGCAATGCACCATTCACCAAATAAATTCGGCTGACCCAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGac                                                                                                                                        <  2:511978/150‑1 (MQ=255)           aTTAGCGCCAGATCAGTATCAGCAATGCACCATTCACCAAATAAATTCGGCTGACCCAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGacaa                                                                                                                                      <  2:458733/151‑1 (MQ=255)              aGCGCCAGATCAGTATCAGCAATGCACCATTCACCAAATAAATTCGGCTGACCCAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGacaaca                                                                                                                                    <  2:57121/150‑1 (MQ=255)                        cAGTATCAGCAATGCACCATTCACCAAATAAATTCGGCTGACCCAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGAc                                                                                                                          <  2:380362/150‑1 (MQ=255)                        cAGTATCAGCAATGCACCATTCACCAAATAAACTCGGCTGACCCAGTACTAACAGATGTTCTGCCATCGCGAACAGTTTCTCCGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGAc                                                                                                                          <  1:57257/150‑1 (MQ=255)                           tATCAGCAATGCACCATTCACCAAATAAATTCGGCTGACCCAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGctc                                                                                                                      >  2:176201/1‑151 (MQ=255)                              cAGCAATGCACCATTCACCAAATAAATTCGGCTGACCCAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTc                                                                                                                   <  2:82983/151‑1 (MQ=255)                                 cAATGCACCATTCACCAAATAAATTCGGCTGACCCAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTcgcg                                                                                                                <  2:672360/151‑1 (MQ=255)                                   aTGCACCATTCACCAAATAAATTCGGCTGACCCAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCgg                                                                                                               >  1:83778/1‑150 (MQ=255)                                     gCACCATTCACCAAATAAATTCGGCTGACCCAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATg                                                                                                            <  2:800168/151‑1 (MQ=255)                                             cACCAAATAAATTCGGCTGACCCAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCa                                                                                                     <  2:846566/150‑1 (MQ=255)                                              aCCAAATAAATTCGGCTGACCCAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATc                                                                                                      >  1:478681/1‑148 (MQ=255)                                                  aataaatTCGGCTGACCCAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACTCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGc                                                                                               >  2:725946/1‑151 (MQ=255)                                                      aatTCGGCTGACCCAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTgcg                                                                                           <  1:214641/151‑1 (MQ=255)                                                         tCGGCTGACCCAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTgcgc                                                                                          >  2:459541/1‑149 (MQ=255)                                                         tCGGCTGACCCAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCa                                                                                         <  2:372787/150‑1 (MQ=255)                                                            gCTGACCCAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAG‑GGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGcc                                                                                      >  1:12544/1‑149 (MQ=255)                                                                aCCCAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGcc                                                                                 >  1:717735/1‑151 (MQ=255)                                                                 cccAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGcc                                                                                 >  1:696554/1‑150 (MQ=255)                                                                 cccAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCt                                                                                <  2:82344/151‑1 (MQ=255)                                                                   cAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTg                                                                               >  2:839241/1‑150 (MQ=255)                                                                          aaCAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTc                                                                       <  1:77716/151‑1 (MQ=255)                                                                           aCAAATGTTCTGCCATCGCGAACAGTTTCCCTGCACTGGCTTTTCCCTCGGCCGTTAGGGGCGCTTTTTTCACCCCCCCAAAGACAACATCCGTCGGTCCCTCTTCGCGGATGGGCATCCGGTCGCTGCGCAGCCAGGCCTGTATCTGt                                                                        >  1:647866/1‑149 (MQ=255)                                                                            cAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGt                                                                     >  1:819724/1‑151 (MQ=255)                                                                               aTGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGc                                                                   >  1:252245/1‑150 (MQ=255)                                                                                  ttCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCAc                                                                 <  2:787101/149‑1 (MQ=255)                                                                                             cgAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTc                                                    >  1:417381/1‑151 (MQ=255)                                                                                                   gTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATc                                              >  1:686168/1‑151 (MQ=255)                                                                                                    tttCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATc                                              >  1:623893/1‑150 (MQ=255)                                                                                                         ctGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAg                                           <  2:56209/148‑1 (MQ=255)                                                                                                           gCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGAt                                      <  1:260471/151‑1 (MQ=255)                                                                                                           gCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGATCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCgg                                        <  1:237413/149‑1 (MQ=255)                                                                                                                  cTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGCCGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACg                               >  1:141596/1‑151 (MQ=255)                                                                                                                    tttCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACTTCCGTCCGACGCTCCTCGCGCATGGGCATCAGATCCCTGCACAGCCAGGCCTGAATCTGCCGTGCACGCGCACGATTTTCTAAATCACGCGGGTAAATACGtt                             >  1:208003/1‑151 (MQ=255)                                                                                                                    tttCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACg                               <  1:226513/149‑1 (MQ=255)                                                                                                                    tttCCCTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGtt                             >  1:520970/1‑151 (MQ=255)                                                                                                                       cccTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTccc                          >  2:706120/1‑151 (MQ=255)                                                                                                                        ccTCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTccc                          >  1:345810/1‑150 (MQ=255)                                                                                                                          tCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAgg                       >  2:288114/1‑151 (MQ=255)                                                                                                                          tCGGCCGTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAgg                       >  2:164467/1‑151 (MQ=255)                                                                                                                                gTTAGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTg                 <  2:1761/151‑1 (MQ=255)                                                                                                                                   aGTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGgcg              >  2:49197/1‑151 (MQ=255)                                                                                                                                    gTGGCGCTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGgcga             <  1:268970/151‑2 (MQ=255)                                                                                                                                       gcgcTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCaa           >  1:700012/1‑150 (MQ=255)                                                                                                                                       gcgcTTCTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCaa           >  2:756049/1‑150 (MQ=255)                                                                                                                                         gcTTTTTTCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATc        <  1:498122/151‑1 (MQ=255)                                                                                                                                           ttttttCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCg       >  1:70358/1‑150 (MQ=255)                                                                                                                                              tttCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATc    <  1:639/150‑1 (MQ=255)                                                                                                                                              tttCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCAGTGGCGCAAATCGATc    <  2:820374/150‑1 (MQ=255)                                                                                                                                               ttCACCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCtt  >  1:294997/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                    GCGGTTAATCATTAGCGCCAGATCAGTATCAGCAATGCACCATTCACCAAATAAATTCGGCTGACCCAGTACTAACAAATGTTCTGCCATCGCGAACAGTTTCTCTGCACTGGCTTTTCCCTCGGCCGTTAGTGGCGCTTTTTTCGCCCCCGCAAAGACAACATCCGTCGGACGCTCTTCGCGGATGGGCATCAGATCGCTGCGCAGCCAGGCCTGAATCTGTCGTGCACGCGCACGATTTTCTAAATCAAGCGGATAAATACGTTCCCAGGTCGGTGGCGCAAATCGATCTT  >  NC_000913/2419955‑2420247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑