Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,437,826	A→G	M9T (ATG→ACG)	pdxB ←	erythronate‑4‑phosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,437,826	0	A	G	100.0%	173.4 / NA	49	M9T (ATG→ACG)	pdxB	erythronate‑4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/24);  total (25/24)

GGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCATATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGTATAAGGATACGTAATGATACAACCTATTTCCGGCC  >  NC_000913/2437678‑2437971                                                                                                                                                     |                                                                                                                                                  gggTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCgta                                                                                                                                                 <  1:217241/151‑1 (MQ=255)  ggTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCgtat                                                                                                                                                >  2:576072/1‑151 (MQ=255)   gTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTAtt                                                                                                                                               <  2:284332/151‑1 (MQ=255)      ttCCTGCCAGCAAAGATTCATTCACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTAtttt                                                                                                                                             <  2:769246/150‑1 (MQ=255)        ccTGCCAGCAAAGATTCATTCACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTtcat                                                                                                                                          <  1:644627/151‑1 (MQ=255)            ccAGCAAAGATTCATTCACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATcaac                                                                                                                                      >  2:707396/1‑151 (MQ=255)              aGCAAAGATTCATTCACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATcaaca                                                                                                                                     >  1:401409/1‑150 (MQ=255)                cAAAGATTCATTCACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAgg                                                                                                                                  <  2:475757/151‑1 (MQ=255)                 aaaGATTCATTCACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAgg                                                                                                                                  >  2:135695/1‑150 (MQ=255)                 aaaGATTCATTCACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAgg                                                                                                                                  >  1:475607/1‑150 (MQ=255)                  aaGATTCATTCACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGAt                                                                                                                                <  2:130287/151‑1 (MQ=255)                    gATTCATTCACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGAttt                                                                                                                              >  2:685281/1‑151 (MQ=255)                     attcattcACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGAtttt                                                                                                                             <  2:756854/151‑1 (MQ=255)                     attcattcACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGAtttt                                                                                                                             <  2:710690/151‑1 (MQ=255)                          ttcaCTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGt                                                                                                                        >  2:516896/1‑151 (MQ=255)                              cTTTCGTGACCGAACGCACCATCAGTGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTtgt                                                                                                                    >  1:714750/1‑151 (MQ=255)                                ttCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTgtgt                                                                                                                  >  2:443150/1‑151 (MQ=255)                                  cGTGACCGAACGCTCCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGtt                                                                                                                 <  1:699212/150‑1 (MQ=255)                                          aaCGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATg                                                                                                        <  1:173256/151‑1 (MQ=255)                                               aCCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACg                                                                                                   >  2:788272/1‑151 (MQ=255)                                                     aGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGAATGGACGCACGGGAACCGCGGGCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGtt                                                                                             >  1:806319/1‑151 (MQ=255)                                                       cgcATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTaa                                                                                           <  2:378121/151‑1 (MQ=255)                                                       cgcATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTa                                                                                            >  2:778062/1‑150 (MQ=255)                                                       cgcAACCGCGTCCGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAAGCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGCTACCTGTATGAGACGAGAGtt                                                                                             >  1:304198/1‑149 (MQ=255)                                                         cATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAAcc                                                                                         <  2:589066/151‑1 (MQ=255)                                                             cgcgTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGAc                                                                                     >  1:816795/1‑151 (MQ=255)                                                               cgTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACa                                                                                    <  2:312675/150‑1 (MQ=255)                                                                gTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACa                                                                                    >  2:412296/1‑149 (MQ=255)                                                                   tGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAgtgt                                                                               >  1:113752/1‑151 (MQ=255)                                                                        gTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCAt                                                                          <  2:396861/151‑1 (MQ=255)                                                                         ttGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCAt                                                                          <  1:708846/150‑1 (MQ=255)                                                                          tGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATa                                                                         >  2:69984/1‑150 (MQ=255)                                                                               gACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTcg                                                                   >  1:52062/1‑151 (MQ=255)                                                                                       ttGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGGCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAg                                                            <  2:816148/150‑1 (MQ=255)                                                                                               ccGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTg                                                   <  1:247680/151‑1 (MQ=255)                                                                                                     aCCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGa                                             <  2:48535/151‑1 (MQ=255)                                                                                                      ccGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGa                                             <  1:16326/150‑1 (MQ=255)                                                                                                      ccGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGAt                                            <  1:605621/151‑1 (MQ=255)                                                                                                        gcgGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGAttt                                          >  2:445117/1‑151 (MQ=255)                                                                                                        gcgGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGAtt                                           <  1:32594/150‑1 (MQ=255)                                                                                                          ggTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTc                                         >  2:351184/1‑150 (MQ=255)                                                                                                          ggTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTc                                         >  1:251862/1‑150 (MQ=255)                                                                                                            tcacctcaccCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAgg                                      >  2:603477/1‑151 (MQ=255)                                                                                                                  caccCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGTATaa                                 <  1:62717/150‑1 (MQ=255)                                                                                                                  caccCAAACGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGTATAAg                                <  2:792355/151‑1 (MQ=255)                                                                                                                        aaCGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGTATAAGGATACg                          <  1:618770/151‑1 (MQ=255)                                                                                                                          cGGCTAAATAAGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGTATAAGGATACGt                         >  2:152153/1‑150 (MQ=255)                                                                                                                                   aaGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGTATAAGGATACGTAATGATACa                >  2:57074/1‑150 (MQ=255)                                                                                                                                    aGTCGCGGGCATAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGTATAAGGATACGTAATGATACAAc              <  1:181229/151‑1 (MQ=255)                                                                                                                                               tAAGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGTATAAGGATACGTAATGATACAACCTATTTCCGGc   >  1:62788/1‑151 (MQ=255)                                                                                                                                                aaGGCGTATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGGATAAGGATACGTAATGATACAACCTATTTCCTGcc  >  1:218698/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                  GGGTTTTCCTGCCAGCAAAGATTCATTCACTTTCGTGACCGAACGCACCATCAGCGCATCCGCGTCTGCCAGTTGAGCGACGGGGATTGGACGCCCGGGAACCGCGGTCACCTCACCCAAACGGCTAAATAAGTCGCGGGCATAAGGCATATTTTCATCAACAAGGATTTTCACGTTTGTGTTACCTGTATGAGACGAGAGTTAACCGGACAAGTGTGCCATAATCTCGCGGCCAGGCATACTTGCGAAGATTTCAGGTATAAGGATACGTAATGATACAACCTATTTCCGGCC  >  NC_000913/2437678‑2437971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑