Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,459,668 T→C T268A (ACG→GCG)  fadI ← beta‑ketoacyl‑CoA thiolase, anaerobic, subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,459,6680TC98.0% 163.2 / NA 49T268A (ACG→GCG) fadIbeta‑ketoacyl‑CoA thiolase, anaerobic, subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (18/30);  minor base G (0/1);  total (18/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

AACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTT  >  NC_000913/2459523‑2459809
                                                                                                                                                 |                                                                                                                                             
aaCATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCg                                                                                                                                          >  1:97848/1‑151 (MQ=255)
     gTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGACCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCg                                                                                                                                     <  2:424295/151‑1 (MQ=255)
         tGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGtt                                                                                                                                 >  1:746154/1‑151 (MQ=255)
          gccagccaTCAATCGCAGTAAATGGGTAGCAGCGCAGATACCCCAGCGGCACCAGCCCTAGTGCTTACGGCCGGGATTCAGTCATCAGGGTCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGttt                                                                                                                                <  1:248934/151‑1 (MQ=255)
          gCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGttt                                                                                                                                <  2:333594/151‑1 (MQ=255)
            cAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGc                                                                                                                              >  1:240271/1‑151 (MQ=255)
            cAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGc                                                                                                                              <  2:599639/151‑1 (MQ=255)
            cAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGc                                                                                                                              <  2:442237/151‑1 (MQ=255)
                    aaTCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAAc                                                                                                                      >  1:614831/1‑151 (MQ=255)
                    aaTCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAAc                                                                                                                      <  1:494197/151‑1 (MQ=255)
                       cGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGcc                                                                                                                   >  2:821396/1‑151 (MQ=255)
                           gTAAATGCGTGGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGgc                                                                                                               >  2:714719/1‑151 (MQ=255)
                               aTGCGTAGCTGCGCAGATCCCCCATCGCCTCCAGCCCTCATCCTTGGGCCCTGGATTCTGTCATCAGGACCGCCGCTGCCGCGCCTTCGGACCGCGGCGTACTGTTTGCCGGCTGTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAg                                                                                                           <  1:411532/151‑1 (MQ=255)
                               aTGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAg                                                                                                           <  2:592679/151‑1 (MQ=255)
                                  cGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCtt                                                                                                        <  1:242024/151‑1 (MQ=255)
                                       cTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCg                                                                                                    >  1:612025/1‑150 (MQ=255)
                                        tGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGt                                                                                                   >  2:149824/1‑150 (MQ=255)
                                         gcgcAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGt                                                                                                   <  2:444525/149‑1 (MQ=255)
                                            cAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCg                                                                                              >  1:400619/1‑151 (MQ=255)
                                                    ccAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGa                                                                                      <  2:364446/151‑1 (MQ=255)
                                                                gCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACcgcg                                                                          <  2:492948/151‑1 (MQ=255)
                                                                 cccTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACcgcg                                                                          >  2:287309/1‑150 (MQ=255)
                                                                    tAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAat                                                                       >  1:431289/1‑150 (MQ=255)
                                                                     aaTTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAatat                                                                     >  1:510103/1‑151 (MQ=255)
                                                                        tCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATAttgt                                                                  >  1:225260/1‑151 (MQ=255)
                                                                           ttCGCCCGGGATTCAGTCATCAGGATCTCCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATAttgttg                                                                <  2:819254/150‑1 (MQ=255)
                                                                            tCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATtgttgt                                                               <  1:717881/150‑1 (MQ=255)
                                                                             cGCCCGGGATTCAGTCATCAGGATCCCCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTc                                                              <  2:857258/150‑1 (MQ=255)
                                                                             cGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATAttgtt                                                                 <  1:843364/147‑1 (MQ=255)
                                                                             cGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTc                                                              <  1:198745/150‑1 (MQ=255)
                                                                              gCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCtt                                                            <  1:24934/151‑1 (MQ=255)
                                                                                   ggATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACa                                                       <  1:149801/151‑1 (MQ=255)
                                                                                          gTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGgtt                                                <  1:338559/151‑1 (MQ=255)
                                                                                                aGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTtat                                          <  1:595792/151‑1 (MQ=255)
                                                                                                aGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTtat                                          >  2:459136/1‑151 (MQ=255)
                                                                                                 ggATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTtat                                          <  1:458869/150‑1 (MQ=255)
                                                                                                  gATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATaa                                        <  2:831932/151‑1 (MQ=255)
                                                                                                     cACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAagg                                      <  2:429185/150‑1 (MQ=255)
                                                                                                      aCCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGACAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAaggag                                    <  2:674005/151‑1 (MQ=255)
                                                                                                       ccGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAaggag                                    <  2:46219/150‑1 (MQ=255)
                                                                                                        cGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAggg                                  <  1:814355/151‑1 (MQ=255)
                                                                                                            gCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATa                               >  2:118159/1‑150 (MQ=255)
                                                                                                            gCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATa                               >  2:708812/1‑150 (MQ=255)
                                                                                                                 gcCATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCa                         <  2:676770/151‑1 (MQ=255)
                                                                                                                  ccATCGGTCAGCGGCGTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAg                        <  2:211265/151‑1 (MQ=255)
                                                                                                                             cggcgTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACctct             >  1:584335/1‑151 (MQ=255)
                                                                                                                              ggcgTACTGTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCtctt            >  1:348914/1‑151 (MQ=255)
                                                                                                                                      gTTTGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGt    <  1:176724/151‑1 (MQ=255)
                                                                                                                                         tGCCGCCGCTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGttt  <  2:771258/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
AACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTT  >  NC_000913/2459523‑2459809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑