Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,478,551 A→G I235M (ATA→ATG)  dsdX → D‑serine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,478,5510AG98.2% 196.0 / ‑4.6 55I235M (ATA→ATG) dsdXD‑serine transporter
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (26/28);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

GATGGCATCACTGATCGGTGGCCCACTTTTCCTTAAATTTCTGGGTCAACGACTGCCCTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATACTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATCTTGGTTGAGTTTATTGGCAACCCTATCACTGCCATGTTTATCGCCGTGTTTGTCGCCTATTATGTGTTGGG  >  NC_000913/2478407‑2478697
                                                                                                                                                |                                                                                                                                                  
gATGGCATCACTGATCGGTGGCCCACTTTTCCTTAAATTTCTGGGTCAACGACTGCCCTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATgctgct                                                                                                                                               <  2:561263/150‑1 (MQ=255)
      aTCACTGATCGGTGGCCCACTTTTCCTTAAATTTCTGGGTCAACGACTGCCCTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCAtt                                                                                                                                        <  2:755252/151‑1 (MQ=255)
          cTGATCGGTGGCCCACTTTTCCTTAAATTTCTGGGTCAACGACTGCCCTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTgcgc                                                                                                                                    >  2:396960/1‑151 (MQ=255)
             aTCGGTGGCCCACTTTTCCTTAAATTTCTGGGTCAACGACTGCCCTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGa                                                                                                                                 <  2:240358/151‑1 (MQ=255)
                ggTGGCCCACTTTTCCTTAAATTTCTGGGTCAACGACTGCCCTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGt                                                                                                                              <  2:545913/151‑1 (MQ=255)
                 gTGGCCCACTTTTCCTTAAATTTCTGGGTCAACGACTGCCCTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGtt                                                                                                                             <  2:527682/151‑1 (MQ=255)
                    gCCCACTTTTCCTTAAATTTCTGGGTCAACGACTGCCCTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAGAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGt                                                                                                                          <  1:55415/151‑1 (MQ=255)
                         cTTTTCCTTAAATTTCTGGGTCAACGACTGCCCTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTaaa                                                                                                                      <  1:799524/150‑1 (MQ=255)
                           tttCCTTAAATTTCTGGGTCAACGACTGCCCTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACg                                                                                                                   >  2:136595/1‑151 (MQ=255)
                                       tCTGGGTCAACGACTGCCCTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAAtt                                                                                                        <  2:721443/150‑1 (MQ=255)
                                       tCTGGGTCAACGACTGCCCTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTg                                                                                                       >  1:178673/1‑151 (MQ=255)
                                          gggTCAACGACTGCCCTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAat                                                                                                    <  2:719148/151‑1 (MQ=255)
                                              cAATGACTGCCCTTTAAACCTGTACCCACAGAATTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTATGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGCTGTTGGTTAAAACGATTGCCGAATTGAata                                                                                                   >  2:26779/1‑148 (MQ=255)
                                              cAACGACTGCCCTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATg                                                                                                 <  1:12739/150‑1 (MQ=255)
                                               aaCGACTGCCCTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACCCTACCGTCATTAGGCGCAACGTTATTCACCAAACTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATTTGgc                                                                                               <  1:800844/151‑1 (MQ=255)
                                               aaCGACTGCCCTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGgc                                                                                               >  2:809478/1‑151 (MQ=255)
                                                aCGACTGCCCTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGgcg                                                                                              >  1:523287/1‑151 (MQ=255)
                                                 cGACTGCCCTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGgcgc                                                                                             >  2:818977/1‑151 (MQ=255)
                                                 cGACTGCCCTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGgcg                                                                                              >  1:511577/1‑150 (MQ=255)
                                                  gACTGCCCTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGgcgc                                                                                             >  1:471151/1‑150 (MQ=255)
                                                     tGCCCTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTg                                                                                          >  1:192831/1‑150 (MQ=255)
                                                      gCCCTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTgag                                                                                        <  2:308171/151‑1 (MQ=255)
                                                      gCCCTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTg                                                                                          >  1:641396/1‑149 (MQ=255)
                                                       cccTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGTTGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTg                                                                                          >  1:797739/1‑148 (MQ=255)
                                                        ccTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTgag                                                                                        >  1:134112/1‑149 (MQ=255)
                                                         cTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGagag                                                                                      >  1:354924/1‑150 (MQ=255)
                                                             aaaCCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGtt                                                                                 <  2:842452/151‑1 (MQ=255)
                                                                  tGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGtat                                                                            >  2:193593/1‑151 (MQ=255)
                                                                   gTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGtat                                                                            <  2:811822/150‑1 (MQ=255)
                                                                   gTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGta                                                                             <  1:320701/149‑1 (MQ=255)
                                                                   gTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGCGGTTTGta                                                                             >  1:175458/1‑149 (MQ=255)
                                                                         acaGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATCTTgg                                                                     <  2:608149/151‑1 (MQ=255)
                                                                            gagTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATCTTGGTTg                                                                  >  1:495890/1‑151 (MQ=255)
                                                                             agTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATCTTGGTTg                                                                  >  1:590008/1‑150 (MQ=255)
                                                                                ttGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATCTTGGTTGAGt                                                               <  1:334826/150‑1 (MQ=255)
                                                                                 tGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATCTTGGTTGAGttt                                                             >  1:391881/1‑151 (MQ=255)
                                                                                         tcAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATCTTGGTTGAGTTTATTGGCa                                                      >  1:253016/1‑150 (MQ=255)
                                                                                           aaaGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATCTTGGTTGAGTTTATTGGCAAcc                                                   <  2:483503/151‑1 (MQ=255)
                                                                                                         aaaaaCACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATCTTGGTTGAGTTTATTGGCAACCCTATCACTGCCATg                                     <  1:75162/151‑1 (MQ=255)
                                                                                                         aaaaaCACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATCTTGGTTGAGTTTATTGGCAACCCTATCACTGCCATg                                     <  2:741159/151‑1 (MQ=255)
                                                                                                            aaCACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATCTTGGTTGAGTTTATTGGCAACCCTATCACTGCCATGttt                                  >  2:296566/1‑151 (MQ=255)
                                                                                                            aaCACTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATCTTGGTTGAGTTTATTGGCAACCCTATCACTGCCATGttt                                  >  2:383810/1‑151 (MQ=255)
                                                                                                              cacTACCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATCTTGGTTGAGTTTATTGGCAACCCTATCACTGCCATGTTTAt                                >  1:158536/1‑151 (MQ=255)
                                                                                                                  aCCGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATCTTGGTTGAGTTTATTGGCAACCCTATCACTGCCATGTTTATCGcc                            >  1:327412/1‑151 (MQ=255)
                                                                                                                   ccGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATCTTGGTTGAGTTTATTGGCAACCCTATCACTGCCATGTTTATCGCCg                           <  2:182268/151‑1 (MQ=255)
                                                                                                                    cGTCATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGATTGGTTTGTATATCTTGGTTGAGTTTATTGGCACCCCTATCACTGCCATGTTTATCGcc                            <  1:827079/149‑1 (MQ=255)
                                                                                                                     gacATTAGGCGCAACGTTAATCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATCTTGGTTGAGTTTATTGGCAACCCTATCACTGCCATGTTTATCGCCg                           <  1:487281/147‑1 (MQ=255)
                                                                                                                      acATTAGCCGCAACGTGATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGAGTGAGAGTGGTTTGTATATCTTGGTTGAGTTTATTGGCAACCCTATCACTGCCATGATTATCGCCgtgt                        >  1:73903/2‑151 (MQ=255)
                                                                                                                       cATTAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATCTTGGTTGAGTTTATTGGCAACCCTATCACTGCCATGTTTATCGCCGTGtt                       <  1:527164/151‑1 (MQ=255)
                                                                                                                          tAGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATCTTGGTTGAGTTTATTGGCAACCCTATCACTGCCATGTTTATCGCCGTGTTTg                     <  2:665288/150‑1 (MQ=255)
                                                                                                                           aGGCGCAACGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATCTTGGTTGAGTTTATTGGCAACCCTATCACTGCCATGTTTATCGCCGTGTTTGt                    <  2:62305/150‑1 (MQ=255)
                                                                                                                                  aCGTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATCTTGGTTGAGTTTATTGGCAACCCTATCACTGCCATGTTTATCGCCGTGTTTGTCGCCtatt            <  1:489139/151‑1 (MQ=255)
                                                                                                                                    gTTATTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATCTTGGTTGAGTTTATTGGCAACCCTATCACTGCCATGTTTATCGCCGTGTTTGTCGCCtattat          <  2:535949/151‑1 (MQ=255)
                                                                                                                                       aTTCACCATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATCTTGGTTGAGTTTATTGGCAACCCTATCACTGCCATGTTTATCGCCGTGTTTGTCGCCTATTAtgt        <  1:721974/150‑1 (MQ=255)
                                                                                                                                            ccATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATCTTGGTTGAGTTTATTGGCAACCCTATCACTGCCATGTTTATCGCCGTGTTTGTCGCCTATTATGTGTTggg  <  2:248003/151‑1 (MQ=255)
                                                                                                                                            ccATGCTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATCTTGGTTGAGTTTATTGGCAACCCTATCACTGCCATGTTTATCGCCGTGTTTGTCGCCTATTATGTGTTggg  >  1:350762/1‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GATGGCATCACTGATCGGTGGCCCACTTTTCCTTAAATTTCTGGGTCAACGACTGCCCTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGCGATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATACTGCTACCCATTGCGCTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATCTTGGTTGAGTTTATTGGCAACCCTATCACTGCCATGTTTATCGCCGTGTTTGTCGCCTATTATGTGTTGGG  >  NC_000913/2478407‑2478697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑