Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,488,952 T→C S73G (AGT→GGT)  yfdE ← acetyl‑CoA:oxalate CoA‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,488,9520TC97.9% 152.8 / ‑6.3 47S73G (AGT→GGT) yfdEacetyl‑CoA:oxalate CoA‑transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (32/14);  minor base G (1/0);  total (33/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

CGGGTTGATTTCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACTCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTC  >  NC_000913/2488806‑2489098
                                                                                                                                                  |                                                                                                                                                  
cgGGTTGATTTCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACAccctct                                                                                                                                                >  1:423864/1‑151 (MQ=255)
     tGATTTCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACCCCCTCTTTGc                                                                                                                                            <  1:15949/150‑1 (MQ=255)
      gATTTCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCa                                                                                                                                          <  2:361797/151‑1 (MQ=255)
        tttCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATg                                                                                                                                        >  2:581682/1‑151 (MQ=255)
         ttCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATg                                                                                                                                        >  2:202384/1‑150 (MQ=255)
          tCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGAt                                                                                                                                      >  1:462273/1‑151 (MQ=255)
           ctagAAGTGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAATATTCTCAGCTAATACATCAGCTTGCTTGACCATCTTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGAtt                                                                                                                                     >  1:687162/4‑151 (MQ=255)
                aGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATtaataa                                                                                                                                <  1:738427/151‑1 (MQ=255)
                      tCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGt                                                                                                                          >  2:771820/1‑151 (MQ=255)
                              aaaCCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGt                                                                                                                  >  1:414119/1‑151 (MQ=255)
                                  cccAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACt                                                                                                              <  1:730916/151‑1 (MQ=255)
                                     aGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGtc                                                                                                           >  2:188601/1‑151 (MQ=255)
                                          ttCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGtccatcc                                                                                                      >  2:50975/1‑151 (MQ=255)
                                           tCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGtccatcc                                                                                                      >  2:773790/1‑150 (MQ=255)
                                             cATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCcac                                                                                                    >  1:682989/1‑150 (MQ=255)
                                                            aaaaTTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAAtgtg                                                                                    >  1:200765/1‑151 (MQ=255)
                                                                 tctcAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGt                                                                               >  1:228327/1‑151 (MQ=255)
                                                                        tAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTatcatc                                                                         >  2:783784/1‑150 (MQ=255)
                                                                           tACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCAcc                                                                      >  2:789764/1‑150 (MQ=255)
                                                                             cATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTTATCATTCATTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACAGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTTCGGGTATCATCTCCAt                                                                    <  1:100946/150‑1 (MQ=255)
                                                                               tCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGAc                                                                 >  2:325768/1‑151 (MQ=255)
                                                                                 aGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGAccc                                                               <  2:414151/151‑1 (MQ=255)
                                                                                   cTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCcgg                                                             >  2:298860/1‑151 (MQ=255)
                                                                                     tGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCcggcg                                                           >  2:366287/1‑151 (MQ=255)
                                                                                     tGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCcggcg                                                           >  1:405177/1‑151 (MQ=255)
                                                                                      gTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCcggcgg                                                          >  2:430853/1‑151 (MQ=255)
                                                                                          gAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTc                                                       <  2:508371/150‑1 (MQ=255)
                                                                                           aGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAAACACATCCTCTTTGCCATGATTATTAAAACTGTAATAGAGAGACTGTCCATCCACATAGGGACCAAATGCACGGGTATCATCACCATGACCCggcggc                                                         >  1:637583/1‑147 (MQ=255)
                                                                                             cATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACt                                                   >  1:713774/1‑151 (MQ=255)
                                                                                                 tttATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTa                                                >  1:446372/1‑150 (MQ=255)
                                                                                                  ttATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTaa                                               <  1:12984/150‑1 (MQ=255)
                                                                                                    ataAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAAtt                                             <  1:413199/150‑1 (MQ=255)
                                                                                                    ataAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAAtt                                             >  1:464471/1‑150 (MQ=255)
                                                                                                    ataAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGAGACTGTCCATCAACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAAtt                                             >  1:855038/1‑150 (MQ=255)
                                                                                                          ataCTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTAc                                           >  2:15582/1‑146 (MQ=255)
                                                                                                          ataCTTTTATCGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTAc                                           <  1:15561/146‑1 (MQ=255)
                                                                                                                    cGTGATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATAtt                            <  1:778178/151‑1 (MQ=255)
                                                                                                                       gATCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTAc                          >  2:728343/1‑150 (MQ=255)
                                                                                                                        aTCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACa                         >  2:408686/1‑150 (MQ=255)
                                                                                                                        aTCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTATCCATCCACATAGGGACCAAAGGTGCGTGTATCATCACCATTACCCGGCGGCTCAGCTTTAATTACCCTTGCGCCCATATTACa                         <  1:265415/150‑1 (MQ=255)
                                                                                                                         tCATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACaa                        >  1:664783/1‑150 (MQ=255)
                                                                                                                          cATTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACaaa                       <  1:303235/150‑1 (MQ=255)
                                                                                                                           aTTCTTTAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACaa                        >  1:855597/1‑148 (MQ=255)
                                                                                                                            agcTTTAAATCAAGAAGCATACCCTGTTTGGAATGATTAGTAAAACTGTAATAGAGTGACTGTCCATCCACATATGGACCAAATGTGCGGGTAAAATCACCATGACAAGGCGGCTCAACTTTAATGAGCATTGCGACCATATTACAaaga                     >  1:664970/3‑150 (MQ=255)
                                                                                                                               tttAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGtt                 >  1:419763/1‑151 (MQ=255)
                                                                                                                                 tAAATCAAGAACCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTg                <  1:193000/150‑1 (MQ=255)
                                                                                                                                          aaCCACACCCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGaa       >  2:515633/1‑150 (MQ=255)
                                                                                                                                              acacGCTCTTTGCAATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCTACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCCAAAGGTc  >  1:487405/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CGGGTTGATTTCTTGAAGCGTTTCCCATGAAAACCCCAGTTTTTCCATTGTACCTGGGCGAAAATTCTCAGCTAATACATCAGCTTGTTTGAGCATATTTATAAATATACTTTTATCGTGATCATTCTTTAAATCAAGAACCACACTCTCTTTGCCATGATTAATAAAACTGTAATAGAGTGACTGTCCATCCACATAGGGACCAAATGTGCGGGTATCATCACCATGACCCGGCGGCTCAACTTTAATTACCCTTGCGCCCATATTACAAAGAAGTTGAGTTCCGAAAGGTC  >  NC_000913/2488806‑2489098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑