Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	327,990	T→C	E248E (GAA→GAG)	betB ←	betaine aldehyde dehydrogenase, NAD‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	327,990	0	T	C	100.0%	156.0 / NA	44	E248E (GAA→GAG)	betB	betaine aldehyde dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/22);  total (22/22)

CGGAACGAAGACGCGGGTGCCATTGGTACACACCTGACCGGAGCTGAAGAAGTTTGCCATCATGGCGATATCGGCGGCGAGATCGAGATCCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACTTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACACCGCCGGTAAATGACACTTTGGCAATGCCCGGATGCTCGGTCAGATATTGCCCGGTCTCCGCGCCCACGCCCGGCAACACGTTAAATACGCCG  >  NC_000913/327849‑328137                                                                                                                                              |                                                                                                                                                    cGGAACGAAGACGCGGGTGCCATTGGTACACACCTGACCGGAGCTGAAGAAGTTTGCCATCATGGCGATATCGGCGGCGAGATCGAGATCCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAgg                                                                                                                                            <  1:304162/151‑1 (MQ=255)       gAAGACGCGGGTGCCATTGGTACACACCTGACCGGAGCTGAAGAAGTTTGCCATCATGGCGATATCGGCGGCGAGATCGAGATCCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAagag                                                                                                                                      <  1:460140/151‑1 (MQ=255)       gAAGACGCGGGTGCCATTGGTACACACCTGACCGGAGCTGAAGAAGTTTGCCATCATGGCGATATCGGCGGCGAGATCGAGATCCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAagag                                                                                                                                      <  1:230982/151‑1 (MQ=255)       gAAGACGCGGGTGCCATTGGTACACACCTGACCGGAGCTGAAGAAGTTTGCCATCATGGCGATATCGGCGGCGAGATCGAGATCCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAag                                                                                                                                        >  2:288993/1‑149 (MQ=255)        aaGACGCGGGTGCCATTGGTACACACCTGACCGGAGCTGAAGAAGTTTGCCATCATGGCGATATCGGCGGCGAGATCGAGATCCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAagag                                                                                                                                      >  1:224197/1‑150 (MQ=255)         aGACGCGGGTGCCATTGGTACACACCTGACCGGAGCTGAAGAAGTTTGCCATCATGGCGATATCGGCGGCGAGATCGAGATCCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGgc                                                                                                                                    <  1:630301/151‑1 (MQ=255)          gACGCGGGTGCCATTGGTACACACCTGACCGGAGCTGAAGAAGTTTGCCATCATGGCGATATCGGCGGCGAGATCGAGATCCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGgcc                                                                                                                                   >  1:261569/1‑151 (MQ=255)            cgcgGGTGCCATTGGTACACACCTGACCGGAGCTGAAGAAGTTTGCCATCATGGCGATATCGGCGGCGAGATCGAGATCCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGgc                                                                                                                                    <  2:209091/148‑1 (MQ=255)            cgcgGGTGCCATTGGTACACACCTGACCGGAGCTGAAGAAGTTTGCCATCATGGCGATATCGGCGGCGAGATCGAGATCCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGgc                                                                                                                                    >  1:209068/1‑148 (MQ=255)              cgGGTGCCATTGGTACACACCTGACCGGAGCTGAAGAAGTTTGCCATCATGGCGATATCGGCGGCGAGATCGAGATCCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGccgccg                                                                                                                               >  2:78310/1‑151 (MQ=255)                     cATTGGTACACACCTGACCGGAGCTGAAGAAGTTTGCCATCATGGCGATATCGGCGGCGAGATCGAGATCCGCATCATCGATAACGAACAGCGGTGATTTACCGGCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGc                                                                                                                        >  1:320890/1‑151 (MQ=255)                     cATTGGTACACACCTGACCGGAGCTGAAGAAGTTTGCCATCATGGCGATATCGGCGGCGAGATCGAGATCCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGc                                                                                                                        <  2:362585/151‑1 (MQ=255)                                 ccTGACCGGAGCTGAAGAAGTTTGCCATCATGGCGATATCGGCGGCGAGATCGAGATCCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACtttttt                                                                                                            <  1:317724/151‑1 (MQ=255)                                        ggAGCTGAAGAAGTTTGCCATCATGGCGATATCGGCGGCGAGATCGAGATCCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTg                                                                                                     >  2:539209/1‑151 (MQ=255)                                                agaagTTTGCCATCATGGCGATATCGGCGGCGAGATCGAGATCCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACAcc                                                                                             <  2:580728/151‑1 (MQ=255)                                                agaagTTTGCCATCATGGCGATATCGGCGGCGAGATCGAGATCCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACAcc                                                                                             >  2:536357/1‑151 (MQ=255)                                                  aagTTTGCCATCATGGCGATATCGGCGGCGAGATCGAGATCCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACAccgc                                                                                           >  2:559965/1‑151 (MQ=255)                                                    gTTTGCCATCATGGCGATATCGGCGGCGAGATCGAGATCCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACAccgccg                                                                                         <  1:230778/151‑1 (MQ=255)                                                      ttGCCATCATGGCGATATCGGCGGCGAGATCGAGATCCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACACCGCCgg                                                                                        <  1:142568/150‑1 (MQ=255)                                                             catGGCGATATCGGCGGCGAGATCGAGATCCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACACCGCCGGTAAATGac                                                                                <  1:51290/151‑1 (MQ=255)                                                                 gCGATATCGGCGGCGAGATCGAGATCCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACACCGCCGGTAAATGACACtt                                                                            <  2:313713/151‑1 (MQ=255)                                                                     tatCGGCGGCGAGATCGAGATCCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCCCCTCTTTCAGGGAAGAGGCCGCCGTGTTTGCCATCACTTTTTTGCCGCTTGCGACACCGCCGGTAAATGACACTTTTGc                                                                        >  1:475426/1‑151 (MQ=255)                                                                          gcggcgAGATCGAGATCCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACACCGCCGGTAAATGACACTTTGGCAATGc                                                                   <  1:279646/151‑1 (MQ=255)                                                                            ggcgAGATCGAGATCCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACACCGCCGGTAAATGACACTTTGGCAATGcc                                                                  <  2:626645/150‑1 (MQ=255)                                                                               gagatcgagatcCGCATCATCGAAAACGATCAGCGGTGATTTCCCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCGGGCGACACCGCCGGTAAATGACACTTTGGCAATGCCCgg                                                               <  2:75245/150‑1 (MQ=255)                                                                                agatcgagatcCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACACCGCCGGTAAATGACACTTTGGCAATGCCCGGAt                                                             >  1:62940/1‑151 (MQ=255)                                                                                    cgagatcCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACACCGCCGGTAAATGACACTTTGGCAATGCCCGGATGCt                                                          <  2:275057/150‑1 (MQ=255)                                                                                          ccGCATCATCGAAAACGATCGGCGGTGATTTACCGCCCAGTTCCATGGTCGCCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACACCGCCGGTAAATGACACTTTGGCAATGCCCGGATGCTCgg                                                       <  1:168984/147‑1 (MQ=255)                                                                                             catcatCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACACCGCCGGTAAATGACACTTTGGCAATGCCCGGATGCTCGGTCAGata                                                <  1:707197/151‑1 (MQ=255)                                                                                              atcatcGAAAACGATCAGCGGTGATTTACCGCCCAGTCCCATGGTCCCCTCTTTCAGGGAAGAGGCCGCCGATTTAGCCATCACTTTTTTGCCGCTGGCGCCACCGCCGGTAAATGACACTTTGGCAATGCCCGGATGCTCGGTCAGata                                                <  1:208960/150‑1 (MQ=255)                                                                                                catcGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACACCGCCGGTAAATGACACTTTGGCAATGCCCGGATGCTCGGTCAGATATTg                                             >  1:559905/1‑151 (MQ=255)                                                                                                     aaaaCGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACACCGCCGGTAAATGACACTTTGGCAATGCCCGGATGCTCGGTCAGATATTGCCCgg                                        >  1:584868/1‑151 (MQ=255)                                                                                                     aaaaCGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACACCGCCGGTAAATGACACTTTGGCAATGCCCGGATGCTCGGTCAGATATTGCCCgg                                        >  2:163269/1‑151 (MQ=255)                                                                                                         cGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACACCGCCGGTAAATGACACTTTGGCAATGCCCGGATGCTCGGTCAGATATTGCCCGGtctc                                    >  1:754009/1‑151 (MQ=255)                                                                                                         cGATCAGCGGTGATTTACCGCCCAGTGCCATGGTCACCTCTTCCAGGAAAGAGGCCGCCGAGTTAGCCATCCCTTTTTTGCCGCGGGCGCCACCGCCGGTAAATGACACTTTGGCAATGCCCGGATGCTCGGTCAGATATTGCCCGGtct                                     <  2:687728/150‑1 (MQ=255)                                                                                                          gATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACACCGCCGGTAAATGACACTTTGGCAATGCCCGGATGCTCGGTCAGATATTGCCCGGTCTcc                                   >  1:713052/1‑151 (MQ=255)                                                                                                           aTCAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACACCGCCGGTAAATGACACTTTGGCAATGCCCGGATGCTCGGTCAGATATTGCCCGGTCTcc                                   <  2:491762/150‑1 (MQ=255)                                                                                                             cAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACACCGCCGGTAAATGACACTTTGGCAATGCCCGGATGCTCGGTCAGATATTGCTCGGTCTCcgc                                 >  1:5517/1‑150 (MQ=255)                                                                                                             cAGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACACCGCCGGTAAATGACACTTTGGCAATGCCCGGATGCTCGGTCAGATATTGCCCGGTCTCcgcg                                >  1:145309/1‑151 (MQ=255)                                                                                                              aGCGGTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACACCGCCGGTAAATGACACTTTGGCAATGCCCGGATGCTCGGTCAGATATTGCCCGGTCTCCgcgc                               >  2:355438/1‑151 (MQ=255)                                                                                                                 ggTGATTTACCGCCCAGTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACACCGCCGGTAAATGACACTTTGGCAATGCCCGGATGCTCGGTCAGATATTGCCCGGTCTCCGCGccc                             >  1:323573/1‑150 (MQ=255)                                                                                                                                 gTTCCATGGTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACACCGCCGGTAAATGACACTTTGGCAATGCCCGGATGCTCGGTCAGATATTGCCCGGTCTCCGCGCCCACGCCCGGCAACACGtt            >  2:328871/1‑151 (MQ=255)                                                                                                                                        ggTCACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACACCGCCGGTAAATGACACTTTGGCAATGCCCGGATGCTCGGTCAGATATTGCCCGGTCTCCGCGCCCACGCCCGGCAACACGTTAAATACg     <  1:163246/151‑1 (MQ=255)                                                                                                                                           cACCTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACACCGCCGGTAAATGACACTTTGGCAATGCCCGGATGCTCGGTCAGATATTGCCCGGTCTCCGCGCCCACGCCCGGCAACACGTTAAATACGCCg  >  1:704508/1‑151 (MQ=255)                                                                                                                                              |                                                                                                                                                    CGGAACGAAGACGCGGGTGCCATTGGTACACACCTGACCGGAGCTGAAGAAGTTTGCCATCATGGCGATATCGGCGGCGAGATCGAGATCCGCATCATCGAAAACGATCAGCGGTGATTTACCGCCCAGTTCCATGGTCACTTCTTTCAGGGAAGAGGCCGCCGAGTTAGCCATCACTTTTTTGCCGCTGGCGACACCGCCGGTAAATGACACTTTGGCAATGCCCGGATGCTCGGTCAGATATTGCCCGGTCTCCGCGCCCACGCCCGGCAACACGTTAAATACGCCG  >  NC_000913/327849‑328137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑