Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,527,658	T→C	V238A (GTT→GCT)	yfeH →	putative inorganic ion transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,527,658	0	T	C	100.0%	180.5 / NA	51	V238A (GTT→GCT)	yfeH	putative inorganic ion transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/26);  total (25/26)

CGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGTTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTT  >  NC_000913/2527514‑2527796                                                                                                                                                 |                                                                                                                                           cgcgCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCtcttct                                                                                                                                      >  1:269962/1‑151 (MQ=255) cgcgCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCtcttct                                                                                                                                      >  2:404849/1‑151 (MQ=255) cgcgCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCtcttct                                                                                                                                      <  1:304250/151‑1 (MQ=255)        tAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCt                                                                                                                                  >  2:275526/1‑148 (MQ=255)         aaaaaaTGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCg                                                                                                                              >  2:713038/1‑151 (MQ=255)          aaaaaTGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGt                                                                                                                             >  2:312786/1‑151 (MQ=255)            aaaTGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGa                                                                                                                           <  1:373345/151‑1 (MQ=255)                              gACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCttt                                                                                                          <  2:259721/150‑1 (MQ=255)                                ccAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATg                                                                                                       <  1:466515/151‑1 (MQ=255)                                ccAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATg                                                                                                       <  1:682596/151‑1 (MQ=255)                                   gACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGc                                                                                                     >  1:302009/1‑150 (MQ=255)                                   gACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCa                                                                                                    <  1:306095/151‑1 (MQ=255)                                     cGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACg                                                                                                  <  2:667516/151‑1 (MQ=255)                                         cATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCg                                                                                               >  1:59705/1‑150 (MQ=255)                                               gTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGc                                                                                        >  2:267320/1‑151 (MQ=255)                                                ttGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCt                                                                                       >  2:348606/1‑151 (MQ=255)                                                     ggtTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAAt                                                                                  >  1:786480/1‑151 (MQ=255)                                                          ataCAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTGACGTCTTTATGGCACGCCGACTGAGCTTCAATAAgg                                                                              <  1:3438/150‑1 (MQ=255)                                                          ataCAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACTTCTTTATGGCACGCCGACTGAGCTTCAATAAGGc                                                                             <  1:850637/151‑1 (MQ=255)                                                            aCAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAg                                                                           <  2:656105/151‑1 (MQ=255)                                                             cAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCa                                                                            <  1:174547/149‑1 (MQ=255)                                                               gCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATg                                                                        <  1:233077/151‑1 (MQ=255)                                                                cGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATg                                                                        >  1:17093/1‑150 (MQ=255)                                                                 gTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGaa                                                                      <  2:419317/151‑1 (MQ=255)                                                                  ttCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGaa                                                                      >  1:325053/1‑150 (MQ=255)                                                                    cAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAAtt                                                                   <  1:779746/151‑1 (MQ=255)                                                                       cGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACt                                                                >  2:258829/1‑151 (MQ=255)                                                                         aaGCCGTCGTTAATGGTATCTGGCATAAAGTTTGCTGGTTATCATTGCTGTTTATCGTGGTACTCAGCTGCGCTCTTCTGGCTATCGTGATTCTAGTTAACGTCTTTATTGCACACCGACCGAGCTTCAATAAGGCAGATGAAATTTCTAt                                                              <  1:566850/151‑1 (MQ=255)                                                                             cgtcgtTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTc                                                          >  1:67688/1‑151 (MQ=255)                                                                                 gtTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCtttt                                                      <  1:606850/151‑1 (MQ=255)                                                                                  ttAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTtg                                                     >  2:357487/1‑151 (MQ=255)                                                                                   tAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTtgt                                                    >  1:490858/1‑151 (MQ=255)                                                                                   tAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTtgt                                                    >  2:592337/1‑151 (MQ=255)                                                                                     aTGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTgg                                                  <  2:644041/151‑1 (MQ=255)                                                                                      tGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGt                                                 >  1:575471/1‑151 (MQ=255)                                                                                          aTCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCg                                              <  2:243091/150‑1 (MQ=255)                                                                                              ggCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCa                                                                            >  1:277486/1‑116 (MQ=255)                                                                                              ggCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCa                                                                            <  2:277511/116‑1 (MQ=255)                                                                                              ggCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGaa                                            <  2:853123/148‑1 (MQ=255)                                                                                              ggCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGaa                                            <  1:536576/148‑1 (MQ=255)                                                                                               gCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAag                                        <  2:700799/151‑1 (MQ=255)                                                                                                 aTAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAagag                                      <  2:383930/151‑1 (MQ=255)                                                                                                   aaaGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGt                                     <  1:384826/150‑1 (MQ=255)                                                                                                        tGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGc                                >  1:467082/1‑150 (MQ=255)                                                                                                                    aTTGCTGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCg                   >  2:53543/1‑151 (MQ=255)                                                                                                                         tGTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGc              >  2:104367/1‑151 (MQ=255)                                                                                                                          gTTTATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCa             <  1:160082/151‑1 (MQ=255)                                                                                                                             tATCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCaaa           <  2:567640/150‑1 (MQ=255)                                                                                                                              aTCGTGGTGGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAAc          >  2:194963/1‑150 (MQ=255)                                                                                                                                 gtggtgGTCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTc      >  1:595131/1‑151 (MQ=255)                                                                                                                                     tggtCAGCTGCGCTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGtt  >  2:348355/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                           CGCGCAATAAAAAATGGATTGCGAAAACTGACCAGACGTCCATTCTGTTGGTGGTTTATACAGCGTTCAGCGAAGCCGTCGTTAATGGTATCTGGCATAAAGTTGGCTGGGGATCATTGCTGTTTATCGTGGTGGTCAGCTGCGTTCTTCTGGCTATCGTGATTGTAGTTAACGTCTTTATGGCACGCCGACTGAGCTTCAATAAGGCAGATGAAATTACTATCGTCTTTTGTGGTTCGAAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTT  >  NC_000913/2527514‑2527796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑