Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,567,770	G→A	A113V (GCG→GTG)	eutH ←	ethanolamine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,567,770	0	G	A	100.0%	179.6 / NA	48	A113V (GCG→GTG)	eutH	ethanolamine transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/23);  total (25/23)

AATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATAC  >  NC_000913/2567622‑2567917                                                                                                                                                     |                                                                                                                                                    aaTGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCcgcccacc                                                                                                                                                   >  2:524757/1‑151 (MQ=255)                    aGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGga                                                                                                                                   >  2:343646/1‑148 (MQ=255)                        ccAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAgcc                                                                                                                            >  2:427319/1‑151 (MQ=255)                             tAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGGATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGccc                                                                                                                        >  2:466514/1‑150 (MQ=255)                              aaCGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCTCACCAGCTCTTTGGCGCGGAAGAAGCCGCCCa                                                                                                                       >  1:14089/1‑150 (MQ=255)                                 gacgGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGGCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCGCATATc                                                                                                                   <  1:596145/151‑1 (MQ=255)                                       cAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGc                                                                                                             >  2:349568/1‑151 (MQ=255)                                        aGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGcc                                                                                                            <  2:173397/151‑1 (MQ=255)                                         gAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGcc                                                                                                            >  1:37615/1‑150 (MQ=255)                                                   tAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTcc                                                                                                 >  2:30806/1‑151 (MQ=255)                                                     aTGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCgg                                                                                               >  1:246560/1‑151 (MQ=255)                                                       gCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGc                                                                                              <  2:197767/150‑1 (MQ=255)                                                        ccGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCg                                                                                             >  2:556766/1‑150 (MQ=255)                                                             cgcCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACAt                                                                                        <  1:150521/150‑1 (MQ=255)                                                                cACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGAt                                                                                    <  2:472974/151‑1 (MQ=255)                                                                 aCCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATg                                                                                   <  1:677807/151‑1 (MQ=255)                                                                     gAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGtt                                                                               <  1:752013/151‑1 (MQ=255)                                                                         ggAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAgc                                                                            <  1:343623/150‑1 (MQ=255)                                                                          gAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCACATATCGCACGCCAGCAGCGTTCCGGCGAACATCGATGGGCTAgcgc                                                                          >  1:28386/1‑151 (MQ=255)                                                                           aaaaCACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGcc                                                                         >  2:475647/1‑151 (MQ=255)                                                                              acacAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCg                                                                        >  1:749377/1‑149 (MQ=255)                                                                               cacaATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGc                                                                     >  2:849653/1‑151 (MQ=255)                                                                                 caATCCTTTGCCCCCTCCTCGACCCCAGAATTAACCCAGAGTATAGCCCCGCGGCTTCGTCGCCGCCCAACAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCa                                                                    <  2:667228/150‑1 (MQ=255)                                                                                  aaTCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCAt                                                                   >  1:429318/1‑150 (MQ=255)                                                                                    tCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGa                                                                                     >  2:598197/1‑130 (MQ=255)                                                                                    tCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGa                                                                                     <  1:598158/130‑1 (MQ=255)                                                                                     cGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTc                                                                <  1:173046/150‑1 (MQ=255)                                                                                     cGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTc                                                                <  2:303575/150‑1 (MQ=255)                                                                                          gCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAAc                                                          <  2:510054/151‑1 (MQ=255)                                                                                               ataattgACCCGAGAATTAACCCAGAGTATCACCACGCGGCTACGTAGACGCCCAACAGCTCTTTGGCGAGGAAAAAACAGCCCATATCCCACGCCAGCAGTGTTTCGGCGAACATCGATGGGTTAGGGCTGAGCTTTTCCTAAACCGGa                                                      >  1:670982/7‑150 (MQ=255)                                                                                                  atcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaata                                                   >  1:74813/1‑150 (MQ=255)                                                                                                   tcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGaataat                                                 >  1:81599/1‑151 (MQ=255)                                                                                                    cGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt                                                <  1:486959/151‑1 (MQ=255)                                                                                                    cGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt                                                >  1:237394/1‑151 (MQ=255)                                                                                                    cGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAAtt                                                >  2:135413/1‑151 (MQ=255)                                                                                                        ccGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCg                                            >  2:731669/1‑151 (MQ=255)                                                                                                         cGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCgg                                           <  1:273498/151‑1 (MQ=255)                                                                                                           agaATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGcc                                         <  2:246584/151‑1 (MQ=255)                                                                                                              aTTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCg                                       >  2:736145/1‑150 (MQ=255)                                                                                                                tAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCgag                                     <  2:76325/150‑1 (MQ=255)                                                                                                                 aaCCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGaa                                   <  2:368079/151‑1 (MQ=255)                                                                                                                 aaCCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTGAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCgaga                                    <  1:555364/150‑1 (MQ=255)                                                                                                                     cAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTg                               <  2:253099/151‑1 (MQ=255)                                                                                                                     cAGAGTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTg                               <  2:237418/151‑1 (MQ=255)                                                                                                                       gagTATAGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTg                               >  1:379724/1‑149 (MQ=255)                                                                                                                             aGCCACGCGGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGt                        <  1:461812/150‑1 (MQ=255)                                                                                                                                   gcgGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTg                 <  2:657345/151‑1 (MQ=255)                                                                                                                                   gcgGCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTg                 >  2:691932/1‑151 (MQ=255)                                                                                                                                     ggCTACGTCGCCGCCCACCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGcc               >  2:94259/1‑151 (MQ=255)                                                                                                                                                  cccaccAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATAc  >  1:188749/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    AATGCCCGCCAGCACGCCGAGCGCCAGATAACGACGGTCAGAAGGTTCGATAATGCCGAGCGCCACCGGAATGGAAAACACAATCGTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATAC  >  NC_000913/2567622‑2567917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑