Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,576,775 | T→C | N535D (AAC→GAC) | maeB ← | malic enzyme: putative oxidoreductase/phosphotransacetylase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,576,775 | 0 | T | C | 98.3% | 204.8 / ‑4.0 | 59 | N535D (AAC→GAC) | maeB | malic enzyme: putative oxidoreductase/phosphotransacetylase |
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (28/30); total (29/30) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 |
CATGGCACCTGCGGTGTGAACGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCC > NC_000913/2576629‑2576920 | cATGGCACCTGCGGTGTGAACGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACt < 2:845025/150‑1 (MQ=255) aTGGCACCTGCGGTGTGAACGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTg > 1:709205/1‑150 (MQ=255) gCACCTGCGGTGTGAACGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATc > 1:674683/1‑150 (MQ=255) ccTGCGGTGTGAACGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCAgc > 1:329832/1‑150 (MQ=255) ccTGCGGTGTGAACGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAgc > 2:185197/1‑150 (MQ=255) ccTGCGGTGTGAACGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAgc < 2:312932/150‑1 (MQ=255) cTGCGGTGTGAACGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAgcg < 2:458451/150‑1 (MQ=255) gCGGTGTGAACGCCATCGCGATAACCAAAGACGTTTTTCACCACGCTAAAATGTTCATGATAATCTCCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCCTGATCGCGCCGATCACTGTCGGGTCACTGATCAgcgcgc < 1:170663/151‑3 (MQ=255) ggTGTGAACGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGc > 2:119964/1‑151 (MQ=255) gtgAACGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTgc > 1:530313/1‑151 (MQ=255) gtgAACGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTgc < 2:61851/151‑1 (MQ=255) gAACGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTgcg > 2:621172/1‑150 (MQ=255) gAACGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTgcg < 1:436229/150‑1 (MQ=255) ccATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCAATGATCAGCGCCCGCTGCGCCTGtt > 1:783971/1‑151 (MQ=255) cATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTc > 2:155300/1‑151 (MQ=255) gcgATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTg > 1:343733/1‑150 (MQ=255) aTAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTg > 2:769397/1‑151 (MQ=255) aaGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGAcgccgc > 2:621066/1‑151 (MQ=255) cacGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATc > 1:632083/1‑151 (MQ=255) acGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGGCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCt > 2:501798/1‑151 (MQ=255) cGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTg < 1:559541/151‑1 (MQ=255) cTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTgg > 2:540407/1‑150 (MQ=255) aaaTGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGt < 2:825716/151‑1 (MQ=255) aTGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTAt < 2:97032/151‑1 (MQ=255) cATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGt > 1:318505/1‑151 (MQ=255) aTGATAATCCCCCACCGTACCGCAAATCATTGCATCGGCTTCCCCCCGCTGAGCCATGAGCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTc < 1:278852/151‑1 (MQ=255) aTGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTc < 1:147859/151‑1 (MQ=255) gATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTcc < 1:457391/150‑1 (MQ=255) aTCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTAc > 1:83091/1‑151 (MQ=255) aTCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTAc > 2:462219/1‑151 (MQ=255) cacccaccGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTAct < 2:186237/150‑1 (MQ=255) cacccaccGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTAct < 2:434890/150‑1 (MQ=255) cccaccGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCtt < 1:345256/151‑1 (MQ=255) ccaccGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCttt < 1:224478/151‑1 (MQ=255) ccaccGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCttt < 2:452605/151‑1 (MQ=255) caccGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCttt > 1:554451/1‑150 (MQ=255) accGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTa > 1:431295/1‑150 (MQ=255) cGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTaa > 2:851239/1‑149 (MQ=255) cGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAg < 1:707408/151‑1 (MQ=255) tACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATAACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAgcg > 2:764126/1‑151 (MQ=255) aCCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAgcgc < 1:146130/151‑1 (MQ=255) cAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGcggat < 2:410175/151‑1 (MQ=255) cAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGcggat < 2:656595/151‑1 (MQ=255) tCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGcggatcgg > 2:26895/1‑150 (MQ=255) tGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGAtt < 2:347444/149‑1 (MQ=255) tGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTc < 2:17650/150‑1 (MQ=255) gCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGt > 1:186494/1‑151 (MQ=255) aTCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGtt > 2:656122/1‑150 (MQ=255) ggCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTg < 2:532615/151‑1 (MQ=255) ccACGCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGAtct < 1:527893/151‑1 (MQ=255) gCTGAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCaa > 1:753602/1‑150 (MQ=255) gAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAAt < 1:501762/150‑1 (MQ=255) gAACCATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTAATCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAAt < 2:185735/150‑1 (MQ=255) ccATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCaa > 1:673396/1‑150 (MQ=255) cATGATCGCGCCGATCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAAACAACg < 1:265516/151‑1 (MQ=255) tCACTGTCGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCAACGCCCGCTTTGATCTg > 2:662395/1‑151 (MQ=255) tGTCGGGTCCCTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTTACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCAACGCACGCTTTGATCTGCaa < 1:713305/150‑1 (MQ=255) cGGGTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGccc < 1:471128/151‑1 (MQ=255) gggTCACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGccc > 1:624432/1‑150 (MQ=255) | CATGGCACCTGCGGTGTGAACGCCATCGCGATAACCAAAGACATTTTTCACCACGCTAAAATGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCGTTATTGACGATCTCAAAATCAACGCCCGCTTTGATCTGCAAGCCC > NC_000913/2576629‑2576920 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |