Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,581,825	T→C	Q318R (CAG→CGG)	ypfG ←	DUF1176 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,581,825	0	T	C	100.0%	192.0 / NA	55	Q318R (CAG→CGG)	ypfG	DUF1176 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/27);  total (28/27)

CGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAATGTTGCGTTCATCAGTTCCAGTTCATTCGTCTCCTGACCGTTGTTGAACGGC  >  NC_000913/2581679‑2581968                                                                                                                                                   |                                                                                                                                                cgcgTCTTATCCGGCCTACAGCGTCTTATCCGGCTTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGAcc                                                                                                                                             <  2:300205/151‑1 (MQ=255)  gcgTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCg                                                                                                                                            >  2:349578/1‑151 (MQ=255)     tCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTc                                                                                                                                          <  1:839491/150‑1 (MQ=255)        tATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCaa                                                                                                                                        <  2:827573/149‑1 (MQ=255)           ccGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCTACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAgcg                                                                                                                                    <  1:404507/150‑1 (MQ=255)            cGGCCTACAGCGTTTTAGCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGcc                                                                                                                                  <  2:598694/151‑1 (MQ=255)             ggCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGcc                                                                                                                                  >  2:711837/1‑150 (MQ=255)             ggCCTACAGCGTATTTTCCGGCCTTCATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAgc                                                                                                                                     <  2:236414/147‑1 (MQ=255)               ccTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGcc                                                                                                                                  >  2:379201/1‑148 (MQ=255)                     gCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCt                                                                                                                         >  1:42284/1‑151 (MQ=255)                           tATCCGGCCTACATGCCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATg                                                                                                                    <  1:763685/150‑1 (MQ=255)                             tCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGcc                                                                                                                  >  2:846376/1‑150 (MQ=255)                             tCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGcc                                                                                                                  >  2:192381/1‑150 (MQ=255)                                  ccTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATc                                                                                                            >  2:137726/1‑151 (MQ=255)                                       aTGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCtt                                                                                                        >  1:373645/1‑150 (MQ=255)                                           cccGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAAt                                                                                                     <  1:108613/149‑1 (MQ=255)                                            ccGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATcc                                                                                                   >  1:449739/1‑150 (MQ=255)                                            ccGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCcg                                                                                                  <  1:152905/151‑1 (MQ=255)                                             cGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCcgc                                                                                                 <  1:640329/151‑1 (MQ=255)                                                     tGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCttt                                                                                          <  1:129503/150‑1 (MQ=255)                                                      gTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTc                                                                                         <  1:8527/150‑1 (MQ=255)                                                           aCGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTa                                                                                     >  1:418579/1‑149 (MQ=255)                                                           aCGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTa                                                                                     >  1:656713/1‑149 (MQ=255)                                                             gggTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGt                                                                                 <  2:152521/151‑1 (MQ=255)                                                             gggTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGt                                                                                 <  2:69562/151‑1 (MQ=255)                                                             gggTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGt                                                                                 <  1:787915/151‑1 (MQ=255)                                                                tGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCAc                                                                              >  1:99325/1‑151 (MQ=255)                                                                 gATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCAcc                                                                             >  1:755586/1‑151 (MQ=255)                                                                     cacaACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGtt                                                                         >  2:667522/1‑151 (MQ=255)                                                                          cGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCAcg                                                                     <  1:502437/150‑1 (MQ=255)                                                                             gggCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGAt                                                                 <  2:782016/151‑1 (MQ=255)                                                                              ggCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGAtt                                                                <  1:739946/151‑1 (MQ=255)                                                                                 cAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTtct                                                             >  2:398177/1‑151 (MQ=255)                                                                                   ggCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCtca                                                           <  2:204461/151‑1 (MQ=255)                                                                                          ggCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAAt                                                     <  2:665407/150‑1 (MQ=255)                                                                                            cccATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAATGtt                                                  <  1:447907/151‑1 (MQ=255)                                                                                            cccATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAATGtt                                                  >  1:669771/1‑151 (MQ=255)                                                                                              cATGCCAGTTATCGCAGGTGGGTTCTTCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAACGTTGc                                                <  1:379177/151‑1 (MQ=255)                                                                                              cATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATTCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAATGTTGc                                                >  2:567791/1‑151 (MQ=255)                                                                                                   cAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAATGTTGCGTtca                                           <  2:249069/151‑1 (MQ=255)                                                                                                   cAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAATGTTGCGTtc                                            >  1:793458/1‑150 (MQ=255)                                                                                                    aGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAATGTTGCGTtcat                                          >  1:169874/1‑151 (MQ=255)                                                                                                       tATCGCAGGTGGGTTCTGCGGTGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTACTCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAATGTTGCGTtcat                                          >  1:691626/1‑148 (MQ=255)                                                                                                           gCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAATGTTGCGTTCATcagttcc                                   >  1:173151/1‑151 (MQ=255)                                                                                                            cAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAATGTTGCGTTCATcagttcca                                  <  1:156859/151‑1 (MQ=255)                                                                                                            cAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAATGTTGCGTTCATcagttcc                                   >  1:347677/1‑150 (MQ=255)                                                                                                             aGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAATGTTGCGTTCATcagttcc                                   >  1:156325/1‑149 (MQ=255)                                                                                                             aGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAATGTTGCGTTCATcagttcc                                   >  1:367796/1‑149 (MQ=255)                                                                                                                tGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAATGTTGCGTTCATcagttccagttc                              >  1:608961/1‑151 (MQ=255)                                                                                                                 gggTTCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAATGTTGCGTTCATcagttccagttc                              >  2:455115/1‑150 (MQ=255)                                                                                                                     tCTGCGGCGTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAATGTTGCGTTCATCAGTTCCAGTTCATTc                          <  2:52983/150‑1 (MQ=255)                                                                                                                          ggcgTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAATGTTGCGTTCATCAGTTCCAGTTCATTCg                         <  1:434640/146‑1 (MQ=255)                                                                                                                           gcgTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACACGATTTCTCATCAAATGTTGCGTTCATCAGTTCCAGTTCATTCGTCTCCt                   <  1:622146/151‑1 (MQ=255)                                                                                                                            cgTAACGCACCAGGCGAAAACGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAATGTTGCGTTCATCAGTTCCAGTTCATTCGTCTCCTg                  >  1:590024/1‑151 (MQ=255)                                                                                                                                             aaaCGCCGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAATGTTGCGTTCATCAGTTCCAGTTCATTCGTCTCCTGACCGTTGTTGAACGGc  >  2:707582/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                CGCGTCTTATCCGGCCTACAGCGTCTTATCCGGCCTACATGTCCCGCCATTTTGTTTAACGGGTGATCCACAACGTGGGCCAGGCGTCTGGCCCATGCCAGTTATCGCAGGTGGGTTCTGCGGCGTAACGCACCAGGCGAAAACGCTGACCGTCAAAGCGCCAGCGCGCCTGAATGCCACAATCGCTTAATCCGCGCCCTTTCGCTAAGGTCACCAGTTCACGCGATTTCTCATCAAATGTTGCGTTCATCAGTTCCAGTTCATTCGTCTCCTGACCGTTGTTGAACGGC  >  NC_000913/2581679‑2581968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑