Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,607,998	T→C	V361A (GTA→GCA)	hyfF →	hydrogenase 4, membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,607,998	0	T	C	100.0%	187.5 / NA	51	V361A (GTA→GCA)	hyfF	hydrogenase 4, membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/25);  total (26/25)

TCTGCTGGCGTACTCCAGCGTGGAGAACATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTAT  >  NC_000913/2607852‑2608139                                                                                                                                                   |                                                                                                                                              tCTGCTGGCGTACTCCAGCGTGGAGAACATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTg                                                                                                                                           >  1:593333/1‑151 (MQ=255) tCTGCTGGCGTACTCCAGCGTGGAGAACATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGGTCCGGCAATGCACTg                                                                                                                                           >  1:294421/1‑151 (MQ=255)    gctgGCGTACTCCAGCGTGGAGAACATGGGGCTGGTCGCGTTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTTCGGTTCCGGCAATGCACTg                                                                                                                                           <  1:628332/148‑1 (MQ=255)       ggCGTACTCCAGCGTGGAGAACATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAg                                                                                                                                     <  2:729634/151‑1 (MQ=255)       gacGTACTCCGGCGTGGAGAGCATCGGGCGGGTCGCGGTGGAGCTCGTCATTCGCGGGCCTCTGTGAAGTTTTGCCGCGCTGCTGCTCATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACGGCTCaa                                                                                                                                      <  1:532322/148‑1 (MQ=255)               ccAGCGTGGAGAACATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCAc                                                                                                                             >  2:516616/1‑151 (MQ=255)                 aGCGTGGAGAACATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCAcgc                                                                                                                           <  1:709858/151‑1 (MQ=255)                      ggAGAACATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGAt                                                                                                                      <  1:287943/151‑1 (MQ=255)                         gaACATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGAtctc                                                                                                                   >  2:682317/1‑151 (MQ=255)                           aCATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCAcg                                                                                                                            <  2:318077/140‑1 (MQ=255)                           aCATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCAcg                                                                                                                            >  1:318053/1‑140 (MQ=255)                            cATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAAc                                                                                                                <  2:717541/151‑1 (MQ=255)                            cATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAAc                                                                                                                <  1:534162/151‑1 (MQ=255)                             aTGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAAAGATGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAAcg                                                                                                               >  2:265059/1‑151 (MQ=255)                                gggCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAAcgtcg                                                                                                            <  1:351074/151‑1 (MQ=255)                                gggCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAAcgtcg                                                                                                            <  1:195916/151‑1 (MQ=255)                                  gCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGGTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAAcgtcgt                                                                                                           >  1:270652/1‑150 (MQ=255)                                   cTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCt                                                                                                         >  1:773566/1‑151 (MQ=255)                                         gcgGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGa                                                                                                   <  1:291215/151‑1 (MQ=255)                                               gAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCa                                                                                             <  2:388355/151‑1 (MQ=255)                                                   tAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAAt                                                                                         >  1:275002/1‑151 (MQ=255)                                                    aGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATc                                                                                        >  2:460310/1‑151 (MQ=255)                                                                 ccGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTAccg                                                                           <  2:626469/151‑1 (MQ=255)                                                                        gAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCt                                                                    >  2:734850/1‑151 (MQ=255)                                                                         aaTTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTg                                                                   <  2:833886/151‑1 (MQ=255)                                                                          aTTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGt                                                                  >  1:57994/1‑151 (MQ=255)                                                                           tttttGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGtt                                                                 >  1:528029/1‑151 (MQ=255)                                                                                gCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTggcg                                                            <  2:591965/151‑1 (MQ=255)                                                                                  cgcgCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTggcggc                                                          <  2:24898/151‑1 (MQ=255)                                                                                      ctgctgCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGt                                                       <  1:648015/150‑1 (MQ=255)                                                                                       tgctgcACATCTTAAACCACAGTCTGGCAAAACCGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACTTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGcggcgg                                                        <  1:555355/148‑1 (MQ=255)                                                                                       tgctgcACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgc                                                     >  1:760097/1‑151 (MQ=255)                                                                                          tgcACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgct                                                  >  2:747973/1‑151 (MQ=255)                                                                                           gcACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgctg                                                 >  2:387050/1‑151 (MQ=255)                                                                                              caTCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgctggcg                                              >  2:97884/1‑151 (MQ=255)                                                                                                  ttAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGcgctggcgctgg                                          >  2:329195/1‑151 (MQ=255)                                                                                                      aCCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAgg                                      >  1:468049/1‑151 (MQ=255)                                                                                                        cacaGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAggg                                     >  2:537790/1‑150 (MQ=255)                                                                                                               tGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATgccgcc                             >  1:166492/1‑151 (MQ=255)                                                                                                                 gCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCt                           <  2:281278/151‑1 (MQ=255)                                                                                                                    aaaaCGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTc                         >  2:622517/1‑150 (MQ=255)                                                                                                                      aaCGCTGCTGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAAc                      >  1:180235/1‑151 (MQ=255)                                                                                                                         gctgctGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAtt                   >  2:201607/1‑151 (MQ=255)                                                                                                                         gctgctGTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAtt                   <  2:102403/151‑1 (MQ=255)                                                                                                                            gctgTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAttttt                >  2:305647/1‑151 (MQ=255)                                                                                                                               gTTCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCtt             >  1:200157/1‑151 (MQ=255)                                                                                                                                ttCTGCGGTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCt              <  2:549181/149‑1 (MQ=255)                                                                                                                                      ggTTCCGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGaa       <  1:329172/150‑1 (MQ=255)                                                                                                                                          ccGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTa   <  2:157738/150‑1 (MQ=255)                                                                                                                                          ccGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTa   <  1:249035/150‑1 (MQ=255)                                                                                                                                          ccGGCAATGCACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTAt  <  2:399981/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                              TCTGCTGGCGTACTCCAGCGTGGAGAACATGGGGCTGGTCGCGGTGGAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTAT  >  NC_000913/2607852‑2608139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑