Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,678,816	T→C	E184E (GAA→GAG)	yphF ←	putative sugar ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,678,816	0	T	C	100.0%	158.3 / NA	44	E184E (GAA→GAG)	yphF	putative sugar ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/25);  total (19/25)

CGAGTGTCGCACCGCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGCGTTACCCAGTTTTTTACCAAATTCCAGCGGATCGCCGAC  >  NC_000913/2678668‑2678962                                                                                                                                                     |                                                                                                                                                   cGAGTGTCGCACCGCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAAcctc                                                                                                                                                  >  2:1304/1‑151 (MQ=255)   aGTGTCGCACCGCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAAcctctt                                                                                                                                                <  1:196059/151‑1 (MQ=255)    gtgtCGCACCGCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACctcttc                                                                                                                                               <  1:50124/151‑1 (MQ=255)      gtCGCACCGCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCa                                                                                                                                              <  1:616974/150‑1 (MQ=255)      gtCGCACCGCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCa                                                                                                                                              <  1:298079/150‑1 (MQ=255)              gCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCttt                                                                                                                                      >  2:769866/1‑150 (MQ=255)              gCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTa                                                                                                                                     <  1:117380/151‑1 (MQ=255)              gCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTa                                                                                                                                     <  2:368340/151‑1 (MQ=255)                ccGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACg                                                                                                                                   >  2:248920/1‑151 (MQ=255)                  gATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTc                                                                                                                                 <  1:479845/151‑1 (MQ=255)                    ttCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGc                                                                                                                               >  2:797945/1‑151 (MQ=255)                          cATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGcaca                                                                                                                         <  2:651246/151‑1 (MQ=255)                                  cGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTc                                                                                                                 <  1:87113/151‑1 (MQ=255)                                            cGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCg                                                                                                       >  1:327964/1‑151 (MQ=255)                                             ggCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGc                                                                                                      >  2:859515/1‑151 (MQ=255)                                                  ggAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAAtt                                                                                                  <  2:675648/150‑1 (MQ=255)                                                  ggAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATtg                                                                                                 >  1:29357/1‑151 (MQ=255)                                                    agaTAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATtgat                                                                                               >  1:272939/1‑151 (MQ=255)                                                     gaTAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATtgatg                                                                                              <  2:852335/151‑1 (MQ=255)                                                      aTAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATtgatg                                                                                              >  2:3608/1‑150 (MQ=255)                                                       tAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGAc                                                                                            >  1:337425/1‑151 (MQ=255)                                                        aaTTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACg                                                                                           <  1:248895/151‑1 (MQ=255)                                                            aGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCaa                                                                                       <  2:660648/151‑1 (MQ=255)                                                            aGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCa                                                                                        >  1:647901/1‑150 (MQ=255)                                                                   cACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCg                                                                                 <  1:614057/150‑1 (MQ=255)                                                                       aaCGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTgg                                                                            >  2:773815/1‑151 (MQ=255)                                                                         cGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTc                                                                          >  2:288957/1‑151 (MQ=255)                                                                              aTCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAAttt                                                                     <  1:12922/151‑1 (MQ=255)                                                                               tCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAAtttt                                                                    >  1:663526/1‑151 (MQ=255)                                                                                 gCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTAt                                                                  <  2:735302/151‑1 (MQ=255)                                                                                    ttATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTAtt                                                                 <  1:1304/149‑1 (MQ=255)                                                                                    ttATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTgg                                                               >  2:423873/1‑151 (MQ=255)                                                                                     tATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTgg                                                               <  2:616684/150‑1 (MQ=255)                                                                                          aaaaCAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCaat                                                           <  2:199379/149‑1 (MQ=255)                                                                                          aaaaCAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCa                                                             <  2:135615/147‑1 (MQ=255)                                                                                            aaCAGACCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCaa                                                            <  1:597750/146‑1 (MQ=255)                                                                                                gTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAaataat                                                   <  2:72095/151‑1 (MQ=255)                                                                                                         tGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGc                                            >  1:95645/1‑149 (MQ=255)                                                                                                             tAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGCGtt                                      >  1:363827/1‑151 (MQ=255)                                                                                                                    aaTTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGCGTTACCCAGt                               <  2:704976/151‑1 (MQ=255)                                                                                                                    aaTTTGCGCGCCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGCGTTACCCAGt                               >  1:251922/1‑151 (MQ=255)                                                                                                                             gcCGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGCGTTACCCAGTTTTTTAcc                       >  2:762267/1‑150 (MQ=255)                                                                                                                               cGGGAACGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGCGTTACCCAGTTTTTTACCaaa                    <  2:29379/151‑1 (MQ=255)                                                                                                                                    aCGCGGGATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGCGTTACCCAGTTTTTTACCAAATTCCa               <  2:312120/151‑1 (MQ=255)                                                                                                                                          gATTTTAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGCGTTACCCAGTTTTTTACCAAATTCCAGCGGAt         <  2:214970/151‑1 (MQ=255)                                                                                                                                               tAAAACCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGCGTTACCCAGTTTTTTACCAAATTCCAGCGGATCGcc     <  2:593960/150‑1 (MQ=255)                                                                                                                                                 aaaCCTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGCGTTACCCAGTTTTTTACCAAATTCCAGCGGATCGCCGAc  >  1:487415/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CGAGTGTCGCACCGCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGCGTTACCCAGTTTTTTACCAAATTCCAGCGGATCGCCGAC  >  NC_000913/2678668‑2678962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑